نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، گروه زیستشناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران
2 استادیار، گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه پیام نور، تهران، ایران
3 استادیار، گرو ه زیستشناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران
چکیده
زمینه و هدف: هدف از این مطالعه، بررسی تأثیرات عصاره گیاه سندانی و تمرین بر هورمونهای جنسی در موش‌های صحرایی نر مبتلا به هیپرلیپیدمی بوده است.
مواد و روش‌ها: 35 سر موش صحرایی نر نژاد ویستار به‌صورت تصادفی به پنج گروه هفت‌تایی شامل گروه کنترل سالم (Con)، کنترل هیپرکلسترولمی (Hc)، دریافت‌کننده عصاره سندانی با دوز mg/kg100، (Ext)، گروه تمرین (E)، دریافت‌کننده عصاره و تمرین (E- Ext) تقسیم شدند. تمرین به مدت هشت هفته برای 30 دقیقه و سه جلسه در هفته معادل سرعت 17-14 متر بر دقیقه انجام شد. سپس میزان هورمونهای تستوسترون، هورمون لوتئینی (LH)، هورمون محرک فولیکی (FSH) و پرولاکتین به روش رادیوایمنواسی و کیت‌های هورمونی مورد سنجش قرار گرفت. از آزمون آماری ANOVA برای تعیین تفاوت بین گروه‌ها استفاده شد.
یافته‌ها: نتایج نشان داد اختلاف معنی‌داری بین میزان FSH (p=0/001)، LH (p=0/000)، پرولاکتین (0/000=) و تستوسترون (0/000=) در بین گروه‌ها بود. نتایج آزمون تعقیبی توکی نشان داد میزان FSH ،LH و تستوسترون بین گروه‌های E- Ext با Hc(p=0/000) (p=0/001) (p=0/001) ، Ext (p=0/000) (p=0/000)(p=0/008) و E (p=0/000)(p=0/011) (p=0/015) و میزان پرولاکتین در بین گروه‌های E- Ext با Hc (p=0/015) ، Ext (p=0/046) و E (p=0/012) تفاوت معنی‌داری وجود داشت. نتایج نشان داد که FSH، LH و تستوسترون در گروه E- Ext نسبت به گروه Hc ، افزایش و پرولاکتین، کاهش داشت.
نتیجه‌گیری: به‌نظر می‌رسد که مصرف عصاره گیاه سندانی همراه با تمرین می‌تواند تأثیرات مثبتی روی هورمونهای جنسی در موش‌های مبتلا به هیپرکلسترومی داشته باشد.
کلیدواژه‌ها
عنوان مقاله [English]
Evaluation of the Effects of Anvillea garcinii Extract and Exercise on Sex Hormones in Male Rats with Hyperlipidemia
نویسندگان [English]
- Amir Abbas Minaeifar 1
- Mohamad Hassan Dashti Khavidaki 2
- Fateme Rasekh 3
1 Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran
2 Assistant Professor, Department of Physical Education, Payame Noor University, Tehran, Iran
3 Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran
چکیده [English]
Introduction: The aim of this study was to evaluate the effect of exercise and Anvillea garcinii extract on sex hormones in male rats with hyperlipidemia.
Materials and Methods: 35 male wistar rats randomly divided into five groups (n=7 for each group), including: 1) control group (Con), 2) hypercholesterolemia (Hc), 3) Anvillea garcinii extract (Ext; 100 mg / kg), 4) training group (E), 5) Extract-exercise (E-Ext). Exercise was performed for eight weeks, 30 minutes and three sessions per week equivalent to a speed of 14-17 m / min. Levels of testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and prolactin were measured by radioimmunoassay and hormone kits. ANOVA test was used to determine the differences between groups.
Results: The results showed a significant difference between the levels of FSH, LH, Prolactin and Testosterone between the groups. The results of Tukey post hoc test showed that FSH, LH and Testosterone levels between E-Ext and Hc groups (p = 0.000) (p = 0.001) (p = 0.001), Ext (p = 0.000) (P = 0.000) (p = 0.008) and E (p = 0.000) (p = 0.011) (p = 0.015) and Prolactin level between E-Ext groups with Hc (0.015). There was a significant difference between Ext (p = 0.046) and E (p = 0.012). The results showed all sexual hormones except Prolactin increased in the E-Ext group compared to the Hc group.
Conclusion: Consumption of plant extracts along with exercise can have positive effects on sex hormones.
کلیدواژه‌ها [English]
- Exercise
- Anvillea garcinii extract
- sexual hormones
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