بررسی تأثیرات عصاره گیاه سندانی (Anvillea garcinii) و تمرین ورزشی بر هورمون‌های جنسی موش‌های صحرایی نر مبتلا به هیپرلیپیدمی

نوع مقاله : مقاله پژوهشی

نویسندگان

1 استادیار، گروه زیست‌شناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران

2 استادیار، گروه تربیت بدنی، دانشکده علوم انسانی، دانشگاه پیام نور، تهران، ایران

3 استادیار، گرو ه زیستشناسی، دانشکده علوم پایه، دانشگاه پیام نور، تهران، ایران

چکیده

زمینه و هدف: هدف از این مطالعه، بررسی تأثیرات عصاره گیاه سندانی و تمرین بر هورمون‌های جنسی در موش‌های صحرایی نر مبتلا به هیپرلیپیدمی بوده است.
مواد و روش‌ها: 35 سر موش صحرایی نر نژاد ویستار به‌صورت تصادفی به پنج گروه هفت‌تایی شامل گروه کنترل سالم (Con)، کنترل هیپرکلسترولمی (Hc)، دریافت‌کننده عصاره سندانی با دوز mg/kg100، (Ext)، گروه تمرین (E)، دریافت‌کننده عصاره و تمرین (E- Ext) تقسیم شدند. تمرین به مدت هشت هفته برای 30 دقیقه و سه جلسه در هفته معادل سرعت 17-14 متر بر دقیقه انجام شد. سپس میزان هورمون‌های تستوسترون، هورمون لوتئینی (LH)، هورمون محرک فولیکی (FSH) و پرولاکتین به روش رادیوایمنواسی و کیت‌های هورمونی مورد سنجش قرار گرفت. از آزمون آماری ANOVA برای تعیین تفاوت بین گروه‌ها استفاده شد.
یافته‌ها: نتایج نشان داد اختلاف معنی‌داری بین میزان FSH (p=0/001)، LH (p=0/000)، پرولاکتین (0/000=) و تستوسترون (0/000=) در بین گروه‌ها بود. نتایج آزمون تعقیبی توکی نشان داد میزان FSH ،LH و تستوسترون  بین گروه‌های E- Ext با Hc(p=0/000) (p=0/001) (p=0/001) ، Ext (p=0/000) (p=0/000)(p=0/008) و E (p=0/000)(p=0/011) (p=0/015) و میزان پرولاکتین در بین گروه‌های E- Ext با Hc (p=0/015) ، Ext (p=0/046) و E (p=0/012) تفاوت معنی‌داری وجود داشت. نتایج نشان داد که FSH، LH و تستوسترون در گروه E- Ext نسبت به گروه Hc ، افزایش و پرولاکتین، کاهش داشت.
نتیجه‌گیری: به‌نظر می‌رسد که مصرف عصاره گیاه سندانی همراه با تمرین می‌تواند تأثیرات مثبتی روی هورمون‌های جنسی در موش‌های مبتلا به هیپرکلسترومی داشته باشد.

کلیدواژه‌ها


عنوان مقاله [English]

Evaluation of the Effects of Anvillea garcinii Extract and Exercise on Sex Hormones in Male Rats with Hyperlipidemia

نویسندگان [English]

  • Amir Abbas Minaeifar 1
  • Mohamad Hassan Dashti Khavidaki 2
  • Fateme Rasekh 3
1 Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran
2 Assistant Professor, Department of Physical Education, Payame Noor University, Tehran, Iran
3 Assistant Professor, Department of Biology, Payame Noor University, Tehran, Iran
چکیده [English]

Introduction: The aim of this study was to evaluate the effect of exercise and Anvillea garcinii extract on sex hormones in male rats with hyperlipidemia.
Materials and Methods: 35 male wistar rats randomly divided into five groups (n=7 for each group), including: 1) control group (Con), 2) hypercholesterolemia (Hc), 3) Anvillea garcinii extract (Ext; 100 mg / kg), 4) training group (E), 5) Extract-exercise (E-Ext). Exercise was performed for eight weeks, 30 minutes and three sessions per week equivalent to a speed of 14-17 m / min.  Levels of testosterone, luteinizing hormone (LH), follicle-stimulating hormone (FSH) and prolactin were measured by radioimmunoassay and hormone kits. ANOVA test was used to determine the differences between groups.
Results: The results showed a significant difference between the levels of FSH, LH, Prolactin and Testosterone between the groups. The results of Tukey post hoc test showed that FSH, LH and Testosterone levels between E-Ext and Hc groups (p = 0.000) (p = 0.001) (p = 0.001), Ext (p = 0.000) (P = 0.000) (p = 0.008) and E (p = 0.000) (p = 0.011) (p = 0.015) and Prolactin level between E-Ext groups with Hc (0.015). There was a significant difference between Ext (p = 0.046) and E (p = 0.012). The results showed all sexual hormones except Prolactin increased in the E-Ext group compared to the Hc group.
Conclusion: Consumption of plant extracts along with exercise can have positive effects on sex hormones.

کلیدواژه‌ها [English]

  • Exercise
  • Anvillea garcinii extract
  • sexual hormones
  1. He N, Ye H. Exercise and Hyperlipidemia. Physical Exercise for Human Health: Springer; 2020. p. 79-90.
  2. Karr S. Epidemiology and management of hyperlipidemia. The American journal of managed care. 2017;23(9 Suppl):S139.
  3. Farokhi F, Riazi S. The Effects of Anethum graveolen Seed Hydroalcoholic Extract(ASHE) and xenical on liver tissue of Induced Hypercholesteroleomia male Rats. Journal of Arak University of Medical Sciences. 2018;20(10):74-89.
  4. Galaly SR, Hozayen WG, Amin KA, Ramadan SM. Effects of Orlistat and herbal mixture extract on brain, testes functions and oxidative stress biomarkers in a rat model of high fat diet. Beni-Suef University Journal of Basic and Applied Sciences. 2014;3(2):93-105.
  5. Mozaffarian V., Gharemaninejad F., Narisima S., Kazempour Sh., Jafari E., Lotfi E., Assadi M. Flora of Iran (Asteraceae). Tehran. Research Institute of Forests and Rangeland Press. 144. 2018. (Persian).
  6. Boukemara H, Hurtado-Nedelec M, Marzaioli V, Bendjeddou D, El Benna J, Marie J-C. Anvillea garcinii extract inhibits the oxidative burst of primary human neutrophils. BMC complementary and alternative medicine. 2016;16(1):1-10.
  7. Hatami L, Estakhr J. The Effects of Hydroalcoholic Extract of Matricaria Recutita on the Hormonal Pituitary-Testis Axis and Testis Tissue Changes of Mature Male Rats. Journal of Fasa University of Medical Sciences. 2013;3(1):56-62.
  8. Perveen S, Al-Taweel AM, Yusufoglu HS, Fawzy GA, Foudah A, Abdel-Kader MS. Hepatoprotective and cytotoxic activities of Anvillea garcinii and isolation of four new secondary metabolites. Journal of natural medicines. 2018;72(1):106-17.
  9. Saremi A, Mombeini A. Influence of swimming exercise training on semen quality and oxidative stress status of the testis in obese male rats. Journal of Practical Studies of Biosciences in Sport. 2016;3(6):65-73.
  10. Vaamonde D, Da Silva-Grigoletto ME, Garcia-Manso JM, Barrera N, Vaamonde-Lemos R. Physically active men show better semen parameters and hormone values than sedentary men. Eur J Appl Physiol. 2012;112(9):3267-73.
  11. Gaeini AA, pournemati P, hooshmand moghadam b. Interactive Effect of Saffron Supplementation and Resistance Training on serum levels of sex hormones in young men. Razi Journal of Medical Sciences. 2018;25(171):20-30.
  12. Parastesh M, Heidarianpour A, Bayat M, Saremi A. Effects of resistance training on serum level of reproductive hormones and sperm parameters in type 2 diabetes rats. Journal of Arak University of Medical Sciences. 2016;19(8):26-36.
  13. Salari R, Yousefi M, Ghorbanzadeh H, Jafarinejad Bajestani M. A review of medicinal herbs with estrogenic, progesteronic, and testosteronic properties. The Iranian Journal of Obstetrics, Gynecology and Infertility. 2016;19(36):19-30.
  14. Ghafari M, Banitalebi E, Faramarzi M, Mohebi A. Comparison of Two Intensities of Aerobic Training (low intensity and High Intensity) on Expression of Perlipin 2 Skeletal Muscle, Serum Glucose and Insulin levels in Streptozotocin-Diabetic Rats. Armaghane danesh. 2017;22(3):282-94.
  15. Changizi Ashtiyani S, Zarei A, Taheri S, Ramazani M. Effect of alcoholic extract of Portulaca Oleracea on serum level of thyroid hormones in hypercholestrolemic Rats. Journal of Gorgan University of Medical Sciences. 2015;17(2):52-8.
  16. Wisløff U, Helgerud J, Kemi OJ, Ellingsen Ø. Intensity-controlled treadmill running in rats: V̇ o 2 max and cardiac hypertrophy. American Journal of Physiology-Heart and Circulatory Physiology. 2001;280(3):H1301-H10.
  17. Dashti khavidaki MH, Azamian Jazi A, Banitalebi E, Faramarzi M. Effect of endurance training on protein expression of CGI-58, ATGL and serum levels of insulin and glucose in streptozotocin-induced diabetic rats. The Journal of Shahid Sadoughi University of Medical Sciences. 2018;26(1):85-96.
  18. Handa SS, Khanuja SPS, Longo G, Rakesh DD. Extraction technologies for medicinal and aromatic plants. Trieste (Italy): Earth, Environmental and Marine Sciences and Technologies; 2008.
  19. dashti H, galedari m, siahkuhian m. Acute Testosterone and Cortisol Hormonal Responses to Volume and Strength Resistance Traiining in Untrained Young Males. 2. 2015;6(12):55-64.
  20. Consitt LA, Copeland JL, Tremblay MS. Hormone responses to resistance vs. endurance exercise in premenopausal females. Canadian journal of applied physiology. 2001;26(6):574-87.
  21. Kraemer WJ, Ratamess NA. Hormonal responses and adaptations to resistance exercise and training. Sports medicine. 2005;35(4):339-61.
  22. Schumann M, Walker S, Izquierdo M, Newton RU, Kraemer WJ, Häkkinen K. The order effect of combined endurance and strength loadings on force and hormone responses: effects of prolonged training. European journal of applied physiology. 2014;114(4):867-80.
  23. Gharakhanlou R, Saremi A, Omidfar K, Sharghi S, Gharaati MR. Effect of resistance training on serum myostatin, testosterone and cortisol in young men. 2008.
  24. Ahtiainen JP, Pakarinen A, Kraemer WJ, Hakkinen K. Acute hormonal responses to heavy resistance exercise in strength athletes versus nonathletes. Canadian Journal of Applied Physiology. 2004;29(5):527-43.
  25. Aqababa H, Mirzaee H, Zarei A, Akbarpour B, Changizi Ashtiyani S. Investigating the Effect of Chelidonium majus Alcoholic Extract on Pituitary-Thyroid in Hypercholesterolemia Male Rats. complementary Medicine Journal. 2014;4(1):757-65.
  26. Carlson BM. Human Embryology and Developmental biology E-book: Elsevier Health Sciences; 2018.
  27. Azadbakht M, Baheddini A, Shorideh SM, Naserzadeh A. Effect of Vitex agnus - castus L. leaf and fruit flavonoidal extracts on serum prolactin concentration. Journal of Medicinal Plants. 2005;4(16):56-61.
  28. Seyfoorian M, Nikbakht M, Doostan MR, Fathi Moghaddam H. Influence of Aerobic and Anaerobic Exercise on the Reproductive Hormones, Cortisol and Prolactin in Male. Jundishapur Scientific Medical Journal. 2011;10(5):545-53.
  29. Bagheri Hamzian Olya j, Khadem Ansari mh, Yaghmaei p. The effect of endurance running activities on Prolactin, Testosterone and DHEA-S levels Studies in Medical Sciences. 2011;21(5):391-7.