نوع مقاله : مقاله پژوهشی
نویسندگان
1 استاد دانش آموخته گروه زیستشناسی، واحد شیراز، دانشگاه آزاد اسلامی، شیراز، ایران
2 دانشیار مرکز تحقیقات سوختی و ترمیم زخم، دانشگاه علوم پزشکی شیراز، شیراز، ایران
3 دانشیار گروه آموزشی زیستشناسی، دانشکده علوم، مؤ سسه آموزش عالی زند شیراز، شیراز، ایران
4 .استادیار مرکز تحقیقات سوختی و ترمیم زخم، دانشگاه علوم پزشکی شیراز، شیراز، ایران
چکیده
زمینه و هدف: کانابیس، ماده روانگردانی است که مورد سوءمصرف میلیونها نفر در دنیا قرار میگیرد. با توجه به شیوع ناباروی و مصرف زیاد این ماده در بین جوانان، مطالعه حاضر با هدف بررسی تأثیر عصاره کانابیس بر رشد سلولهای بنیادی مزانشیمی مشتق از آندومتر موشهای صحرایی بالغ انجام گردید.
مواد و روشها: در این مطالعه تجربی، سلولهای بنیادی مزانشیمی از آندومتر رحم موشهای صحرایی استخراج گردید و پس از کشت و تأیید مزانشیمی بودن سلولها با روش فلوسایتومتری و براساس بیان مارکرهای CD34 و CD90و بیان نشدن مارکر CD105، در پاساژ سوم کشت سلولی، تأثیر کانابیس در غلظتهای ng/ml 1000 و 100 در طول مدت 1 الی 8 روز بر میزان رشد این سلولها محاسبه گردید و نتایج با استفاده از آزمونهای ANOVA و توکی آنالیز شدند.
یافتهها: سلولهای جدا شده از آندومتر، 24 ساعت پس از انتقال، کاملاً به کف فلاسک کشت سلولی چسبیدند. مزانشیمی بودن این سلولها با بیان مارکرهای سطحی CD90، CD105 و بیان نشدن مارکر CD34 تأیید گردید. نتایج شمارش سلولی نیز حاکی از رشد سلولهای تیمار شده با کانابیس تا روز سوم تیمار مشابه با گروه کنترل بود و از روز چهارم درگروه تیمار با دوز ng/ml100کانابیس افزایش معناداری در سطح 0/05>p و در گروه تیمار با دوز ng/ml1000 در روز سوم نسبت به گروه کنترل کاهش معنادار و از روز چهارم افزایش معناداری در سطح 0/01>p مشاهده گردید.
نتیجهگیری: نتایج نشان داد سلولهای جدا شده از آندومتر، از نوع بنیادی مزانشیمی بودند و عصاره کانابیس احتمالاً از طریق گیرندههای کانابینوئیدی باعث تحریک رشد این سلولها میگردد.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Evaluation of the Effects of Cannabis on Cell Growth of Stem Cells Derived from Endometrial Tissue of Uterine Rats
نویسندگان [English]
- Najmea Farhadi 1
- Davood Mehrabani 2
- Seyed Ebrahim Hosseini 3
- seyede sara hashemi 4
1 Sc Department of Biology, Shiraz Branch, Islamic Azad University, Shiraz, Iran
2 Associate Professor Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
3 Associate Professor Department of Biology, Faculty of Sciences, Zand Institute of Higher Education, Shiraz, Iran
4 Assistant Professor Burn and Wound Healing Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
چکیده [English]
Introduction: Cannabis is psychoactive substance that is abused by millions of people the world. Due to the high consumption of this substance among young people of reproductive age, the present study was performed to investigate the effect of cannabis on the growth of endometrial mesenchymal stem cells(msc) derived from rats.
Materials and Methods: In this experimental study, msc were extracted from the endometrium of rats and after culturing and confirming the mesenchymal nature of the cells by flowcytometry and by expressingCD34 and CD90 and not expressingCD105 markers,in the third passage of cell culture, the effects of cannabis in concentration of100and1000ng/ml were calculated on the growth of these cells within1to8days and the results were analyzed using ANOVA and Tuki tests.
Results: Cells isolated from the endometrium adhered to the floor of the cell culture flask24hours after transfer. The mesenchymal nature of these cells was confirmed by the expression of CD90, CD105and non-expression of CD34markers.The results ofcell counts also showed the growth of cells treated with cannabis until the third day of treatment similar to the control group. From the fourth day in the treatment group with a dose of100ng/ml cannabis increased significantly top<0.05and in the treatment group with a dose of1000ng/ml. Third, there was significant decrease compared to the control group and from the fourth day, significant increase was observed atp<0.05.
Conclusion: The results showed that the cells isolated from the endometrium were of the msc, and that cannabis probably stimulated the growth of these cells through cannabinoid receptors.
کلیدواژهها [English]
- Cannabis
- endometrium
- stem cells
- cell growth
- rat
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