نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد میکروب شناسی، گروه میکروبشناسی، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، ایران
2 استاد باکتری شناسی پزشکی، مرکز تحقیقات بیماریهای عفونی (IDRC)، دانشگاه علوم پزشکی اراک، اراک، ایران
3 استادیار باکتری شناسی پزشکی، گروه میکروبشناسی، دانشکده پزشکی، دانشگاه علوم پزشکی اراک، ایران
4 دانشیار ژنتیک پزشکی، مرکز تحقیقات بیماریهای عفونی (IDRC)، دانشگاه علوم پزشکی اراک، اراک، ایران
چکیده
زمینه و هدف: تشکیل بیوفیلم یکی از مهمترین مکانیسمهای بقاء و پاتوژنسیته اشرشیاکلی بهویژه در ایزولههای مقاوم به آنتیبیوتیک است. ایزولههای تولیدکننده بتالاکتاماز وسیع الطیف (ESBL) با افزایش مقاومت دارویی و توانایی تشکیل بیوفیلم میتوانند تهدید جدی برای بیماران مبتلا به سرطان کولورکتال ایجاد کنند. این مطالعه باهدف بررسی تولید بیوفیلم ایزولههای ESBL و الگوی مقاومت دارویی انجام شد.
مواد و روشها: تعداد 46 نمونه از مدفوع بیماران مبتلا به سرطان کولورکتال جمعآوری شد. ایزولهها با استفاده از تستهای بیوشیمیایی استاندارد شناسایی گردیدند. تولید ESBL به روش فنوتیپی با استفاده از روش دیسک دیفیوژن طبق دستورالعملCLSI انجام شد. شناسایی فنوتیپی بیوفیلم به روش میکروپلیت انجام شد. حضور ژنهای fimH و Luxs1 با استفاده از واکنش زنجیرهای پلیمراز (PCR) انجام شد.
یافتهها: نتایج نشان داد که 2/65% از نمونه ها قادر به تشکیل بیوفیلم بودند. براساس میزان جذب نوری در آزمون میکروتیتر پلیت 5/19% بیوفیلم قوی، 5/19% بیوفیلم با شدت متوسط و 26% بیوفیلم ضعیف تشکیل دادند. همچنین 7/34% فاقد توانایی تشکیل بیوفیلم بودند. در بررسی ژنوتیپی با استفاده از روش PCR، ژنهای fimH و luxs1 مورد ارزیابی قرار گرفتند که 46 ایزوله دارای ژنهای fimH و luxs1 بودند.
نتیجهگیری: نتایج این مطالعه نشان داد که تشکیل بیوفیلم در ایزولههای E.coli تولیدکننده ESBL در بیماران مبتلا به سرطان کولورکتال شایع است و میتواند نقش مهمی در افزایش مقاومت آنتیبیوتیکی ایفا کند. شناسایی و کنترل این عوامل میتواند نقش مهمی در بهبود روند درمان این بیماران داشته باشد.
تازه های تحقیق
https://scholar.google.com/citations?user=qNydrc8AAAAJ&hl=en
https://www.ncbi.nlm.nih.gov/myncbi/
https://scholar.google.com/citations?hl=en&user=B72SMLQAAAAJ
https://pubmed.ncbi.nlm.nih.gov/?term=Mona+nasaj&sort=
https://scholar.google.com/citations?hl=en&user=7RcVPoAAAAAJ&view_op=list_works&sortby=pubdate
https://pubmed.ncbi.nlm.nih.gov/?term=mohammad+arjomandzadegan%5bAuthor%5d&sort=
https://scholar.google.com/citations?hl=en&user=1fWLpg0AAAAJ
https://pubmed.ncbi.nlm.nih.gov/?term=Azam+ahmadi%5bAuthor%5d&sort=
کلیدواژهها
موضوعات
عنوان مقاله [English]
Phenotypic and Genotypic Study of Biofilm Formation in ESBL-Producing E. coli strains Isolated from Patients with Colorectal Cancer and Its Relationship with Antibiotic Resistance Patterns
نویسندگان [English]
- Sadaf Ataei alamdari 1
- Mohammad Arjomandzadegan 2
- Mona Nassaj Manizani 3
- Azam Ahmadi 4
1 MSC in Medical Microbiology, Department of Microbiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
2 Professor of Medical Bacteriology, Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
3 Assistant Professor of Medical Bacteriology, Department of Microbiology, Faculty of Medicine, Arak University of Medical Sciences, Arak, Iran
4 Associate Professor in Medical Genetics, Infectious Diseases Research Center (IDRC), Arak University of Medical Sciences, Arak, Iran
چکیده [English]
Background: Biofilm formation is one of the most important mechanisms of survival and pathogenicity of Escherichia coli, especially in antibiotic-resistant isolates. Beta-lactamase-producing isolates (ESBL) can pose a serious threat to patients with colorectal cancer due to increased drug resistance and the ability to form biofilms. This study aimed to investigate the biofilm production of ESBL isolates and the pattern of drug resistance.
Materials and Methods: A total of 46 stool samples from colorectal cancer patients were collected. The isolates were identified using standard biochemical tests. The production of ESBL was phenotypically identified using the Disk diffusion method according to CLSI guidelines. Phenotypic identification of biofilm was performed using the microtiter plate method. The presence of fimH and Luxs1 genes was assessed using polymerase chain reaction (PCR).
Results: The results showed that 65.2% were capable of forming biofilms. Based on optical density in the microtiter plate test, 19.5% formed strong biofilms,19.5% formed moderately strong biofilms, and 26% formed weak biofilms. Additionally, 34.7% were unable to form biofilms. In the genotypic analysis using PCR, the fimH and luxs1 genes were evaluated, and the results indicated that a total of 46 isolates possessed the fimH and luxs1 genes.
Conclusion: The results of this study indicated that biofilm formation in ESBL-producing E. coli isolates in colorectal cancer patients is common and may play an important role in increasing antibiotic resistance.
کلیدواژهها [English]
- Escherichia coli
- biofilm
- fimH gene
- Luxs1 gene
- colorectal cancer
- Paterson DL, Bonomo RA. Extended-spectrum β-lactamases: a clinical update. Clinical microbiology reviews. 2005;18(4):657-86.
- Dejea CM, Fathi P, Craig JM, Boleij A, Taddese R, Geis AL, et al. Patients with familial adenomatous polyposis harbor colonic biofilms containing tumorigenic bacteria. Science. 2018;359(6375):592-7.
- Jian C, Yinhang W, Jing Z, Zhanbo Q, Zefeng W, Shuwen H. Escherichia coli on colorectal cancer: A two‐edged sword. Microbial Biotechnology. 2024;17(10):e70029.
- Sharma G, Sharma S, Sharma P, Chandola D, Dang S, Gupta S, et al. Escherichia coli biofilm: development and therapeutic strategies. Journal of applied microbiology. 2016;121(2):309-19.
- Deng Z, Hou K, Valencak TG, Luo XM, Liu J, Wang H. AI-2/LuxS quorum sensing system promotes biofilm formation of Lactobacillus rhamnosus GG and enhances the resistance to enterotoxigenic Escherichia coli in germ-free zebrafish. Microbiology spectrum. 2022;10(4):e00610-22.
- Wong MH, Chan EW, Liu LZ, Chen S. PMQR genes oqxAB and aac (6) Ib-cr accelerate the development of fluoroquinolone resistance in Salmonella typhimurium. Frontiers in microbiology. 2014;5:521.
- Liu C, Li Z, Ding J, Zhen H, Fang M, Nie C. Species-level analysis of the human gut microbiome shows antibiotic resistance genes associated with colorectal cancer. Frontiers in Microbiology. 2021;12:765291.
- procop GW; Church DL; Hall GS; Janda WM; Schreckenberger PC; Woods GL Koneman's color atlas and textbook of diagnostic microbiology. 7th ed. Philadelphia: Wolters Kluwer Health; 2017.
- Performance Standards for Antimicrobial Susceptibility Testing; CLSI supplement M100. 35th ed. ed. Wayne, PA: Clinical and Laboratory Standards Institute(CLSI); 2025.
- Gonzales E, Patiño L, Ore E, Martínez V, Moreno S, Cruzado NB, et al. β-lactamasas de espectro extendido tipo CTX-M en aislamientos clÃnicos de Escherichia coli y Klebsiella pneumoniae en el Instituto Nacional de Salud del Niño-Breña, Lima, Perú. Revista Medica Herediana. 2019;30(4):242-8.
- Ariyanti T, Suhaemi S, Mulyati S, Sukatma S, Sumirah S, Noor SM, et al. Dissemination and phenotypic characterization of ESBL-producing Escherichia coli in Indonesia. Open Veterinary Journal. 2025;15(3):1340.
- Mirzaei R, Yousefimashouf R, Arabestani MR, Sedighi I, Alikhani MY. The issue beyond resistance: Methicillin-resistant Staphylococcus epidermidis biofilm formation is induced by subinhibitory concentrations of cloxacillin, cefazolin, and clindamycin. PLoS One. 2022;17(11):e0277287.
- Abdul Raheem Hasan S, Sajid Al-Jubori S, Abdul Sattar Salman J. Molecular Analysis of fimA Operon Genes among UPEC Local Isolates in Baghdad City. Archives of Razi Institute. 2021;76(4):829-40.
- Alshammari M, Ahmad A, AlKhulaifi M, Al Farraj D, Alsudir S, Alarawi M, et al. Reduction of biofilm formation of Escherichia coli by targeting quorum sensing and adhesion genes using the CRISPR/Cas9-HDR approach, and its clinical application on urinary catheter. Journal of Infection and Public Health. 2023;16(8):1174-83.
- Bakhshi M, Jamali H. Evaluating the Biofilm Ability by Microtiter Plate Method in Uropathogenic Escherichia Coli Isolated from Patients with Urinary Tract Infection with Urinary Stones. Armaghane Danesh. 2020;25(4):474-86.
- Surgers L, Boyd A, Girard P-M, Arlet G, Decré D. Biofilm formation by ESBL-producing strains of Escherichia coli and Klebsiella pneumoniae. International Journal of Medical Microbiology. 2019;309(1):13-8.
- Nouri R, Hasani A, Masnadi Shirazi K, Alivand MR, Sepehri B, Sotoudeh S, et al. Mucosa‐Associated Escherichia coli in Colorectal Cancer Patients and Control Subjects: Variations in the Prevalence and Attributing Features. Canadian Journal of Infectious Diseases and Medical Microbiology. 2021;2021(1):2131787.
- Hatayama N, Sato Y, Tahira R, Hori T, Tansho-Nagakawa S, Ono Y, et al. Cefmetazole, flomoxef, and meropenem are effective against planktonic cells but not biofilms of extended-spectrum β-lactamase-producing Escherichia coli. BMC Microbiol. 2025;25(1):387.
- Garousi M, Tabar SM, Mirazi H, Asgari P, Sabeghi P, Salehi A, et al. A global systematic review and meta-analysis on correlation between biofilm producers and non-biofilm producers with antibiotic resistance in Uropathogenic Escherichia coli. Microbial Pathogenesis. 2022; 164:105412.
- Raskov H, Kragh KN, Bjarnsholt T, Alamili M, Gögenur I. Bacterial biofilm formation inside colonic crypts may accelerate colorectal carcinogenesis. Clinical and translational medicine. 2018;7(1):30.
- Zhang Q, Gao H-Y, Li D, Li Z, Qi S-S, Zheng S, et al. Clinical outcome of Escherichia coli bloodstream infection in cancer patients with/without biofilm formation: a single-center retrospective study. Infection and drug resistance. 2019:359-71.
- Dumych T, Yamakawa N, Sivignon A, Garenaux E, Robakiewicz S, Coddeville B, et al. Oligomannose-rich membranes of dying intestinal epithelial cells promote host colonization by adherent-invasive E. coli. Frontiers in microbiology. 2018;9:742.
- Sun T, Li X-D, Hong J, Liu C, Zhang X-L, Zheng J-P, et al. Inhibitory effect of two traditional Chinese medicine monomers, berberine and matrine, on the quorum sensing system of antimicrobial-resistant Escherichia coli. Frontiers in microbiology. 2019;10:2584.
- Zarei O, Arabestan MR, Majlesi A, Mohammadi Y, Alikhani MY. Determination of virulence determinants of Escherichia coli strains isolated from patients with colorectal cancer compared to the healthy subjects. Gastroenterology and hepatology from bed to bench. 2019;12(1):52.
- Jin Y, Tang S, Li W, Ng SC, Chan MW, Sung JJ, et al. Hemolytic E. coli promotes colonic tumorigenesis in females. Cancer research. 2016;76(10):2891-900.
- Yoshida M, Thiriet-Rupert S, Mayer L, Beloin C, Ghigo J. Selection for nonspecific adhesion is a driver of FimH evolution increasing. Escherichia coli. 2022.
- Xu X, Cai M, Lai H, Lian S, Hu L, Cao Y. Characterization of AI-2/LuxS quorum sensing system in antibiotic resistance, pathogenicity of non carbapenemase-producing carbapenem-resistant Escherichia coli. BMC microbiology. 2025;25(1):140.
- Compaoré M, Kiendrebeogo M. Free radical scavenging capacity and anti-biofilm potentiality of six wild edible fruits from Burkina Faso. Int J Curr Microbiol App Sci. 2018;7(9):2085-93.
- Aydın E, Kocaaga M, Temel A. Association of antibiotic resistance and biofilm formation in Escherichia coli ST131/O25b. Acta microbiologica et immunologica Hungarica. 2024;71(3):197-205.
- Gebremariam T, Eguale T, Belay T, Kalayu AA, Abula T, Engidawork E, et al. Antibiotic Resistance, and Biofilm Forming Characteristics of Escherichia coli Clinical Isolates at a Hospital in Tigray, Northern Ethiopia. Cureus. 2024;16(11).
- Tirono M, Suhariningsih RA, Yasin M, Gunawan A. Combination model of electric field and light for deactivation biofilm bacteria. Instrumentation Mesure Metrologie. 2018;17(1):153-65.
)