نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روان‌شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران
2 دانشیار گروه فیزیولوژی ورزشی، دانشکده علوم تربیتی و روان‌شناسی، دانشگاه محقق اردبیلی، اردبیل، ایران
چکیده
زمینه و هدف: استئوکلسین و استئوپونتین با بیماریهای دیابت و پوکیاستخوان رابطه دارند. هدف، تعیین تأثیر هشت هفته تمرین استقامتی- مقاومتی و مصرف مکمل تورین بر پروتئینهای استئوکلسین و استئوپونتین در موش نر ویستار دیابتی است.
مواد و روش‌ها: در این مطالعه تجربی،40 رت نر ویستار بالغ با دامنه وزنی 300-250 گرم و سن 6 هفته، به‌صورت تصادفی در 3 گروه دیابتیشده (هر گروه 10 رت)، گروه تمرین، گروه مکمل و گروه کنترل و یک گروه غیردیابتی به‌عنوان گروه کنترل سالم قرار گرفتند. برای دیابتیکردن رتها، 55 میلیگرم بر هر کیلوگرم از وزن بدن استرپتوزوسین تزریق شد. قند خون بالای250 میلیگرم بر دسیلیتر در خون به‌عنوان دیابتی درنظر گرفته شد. تمرینات ترکیبی به مدت هشت هفته و 5 بار در هفته انجام شد. مکمل تورین به‌صورت محلول 1 درصد در آب روزانه مصرف شد. برای مقایسه تغییرات بینگروهی و درونگروهی متغیرها، ANOVA یکراهه و تعقیبی بونفرونی استفاده شد.
یافته‌ها: استئوکلسین در گروه تمریندیابتی در مقایسه با کنترلدیابتی افزایش معنیدار (0/018=P) و در گروه کنترلدیابتی در مقایسه با کنترل سالم، کاهش معنی دار داشت (0/0001=P). استئوپونتین در گروه کنترلدیابتی در مقایسه با کنترل‌سالم، افزایش معنی دار داشت (0/003=P) در حالیکه در گروههای دیگر تفاوت معنیداری نداشت (1/000=P). وزن بدن و BMI در گروه تمریندیابتی در مقایسه با مکملدیابتی، افزایش معنیدار داشت (0/0001=P). غذای مصرفی در گروه تمریندیابتی در مقایسه با مکملدیابتی کاهش معنیدار داشت (0/030=P).
نتیجه‌گیری: هشت هفته تمرین استقامتی- مقاومتی و مصرف مکمل تورین منجر به افزایش استئوکلسین و تغییر نکردن استئوپونتین شد.
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Effect of 8 Weeks of Endurance -Resistance Training and Taurine Supplementation on Osteocalcin and Osteopontin Proteins in Diabetic Wistar Rats with STZ
نویسندگان [English]
- Fatemeh Akbari Vargsaran 1
- Ameneh Pourrahim Ghoroghchi 2
1 Masters in Sports Physiology, Dept. of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
2 Associate Prof, Department of Sport Physiology, Faculty of Educational Sciences and Psychology, University of Mohaghegh Ardabili, Ardabil, Iran
چکیده [English]
Introduction: Osteocalcin and osteopontin are related to diabetes and osteoporosis. The aim was to determine the effect of eight weeks of endurance-resistance training and taurine supplementation on osteocalcin and osteopontin in diabetic male Wistar rats.
Materials and Methods: In this experimental study,40adult male Wistar rats weighing 250-300grams and 6weeks old were randomly divided into 3 diabetic groups (each group, 10Rat); exercise, supplement and a control and a non-diabetic groups were included as a healthy control group. To make diabetic, 55mg/kg of body weight of streptozocin was injected. Combined exercises were performed for eight weeks and 5times a week. Taurine supplementation was taken as a 1% solution in water daily. One-way ANOVA and Bonferroni's post hoc were used to compare the inter-group and intra-group changes of the variables.
Results: Osteocalcin increased significantly in the diabetic exercise group compared to the diabetic control (P=0.018) and decreased significantly in the diabetic control compared to the healthy (P=0.0001). Osteopontin increased significantly in the diabetic control group compared to the healthy control (P=0.003); While, there was no significant difference in other groups (P=1.000). Body weight and BMI increased significantly in the exercise-diabetic group compared to the diabetic-supplement (P=0.0001). There was a significant decrease in food consumption in the diabetic exercise group compared to the diabetic supplement group (P=0.030).
Conclusion: Eight weeks of endurance-resistance training and taurine supplementation led to an increase in osteocalcin and no change in osteopontin.
کلیدواژه‌ها [English]
- Combined training
- Taurine supplement
- Osteocalcin
- Osteopontin
- diabetes
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