نوع مقاله : مقاله پژوهشی
نویسندگان
1 گروه زیست شناسی، دبیر زیست شناسی اداره آموزش و پرورش قائمشهر، قائمشهر، ایران
2 هیئت علمی/دانشگاه آزاد اسلامی واحد قائمشهر
چکیده
زمینه و هدف: در چند دهه اخیر دانشمندان بر استفاده از ترکیبات طبیعی برای درمان سرطان، از جمله عصاره خام گیاهی یا برخی از متابولیت‌های ثانویه گیاهی متمرکز شده اند. هدف از این پژوهش بررسی فعالیت سمیت سلولی عصاره اتانولی خام تهیه شده از جوانه برگ و گل گیاه سیاه ولیک Crataegus melanocarpa بر لاین‌های سلولی سرطانی MCF-7 و HeLa.
مواد و روشها: در این پژوهش اثر غلظت‌های مختلف عصاره بر لاین های سلولی MCF-7 و HeLa، با استفاده از روش رنگ‌آمیزی و آزمون MTT تعیین شد. چگالی نوری محلول رنگی در طول موج 570 نانومتر توسط دستگاه الایزا ریدر، پس از 24 و 48 و 72 ساعت انکوباسیون، تعیین شد.
یافته ها: نتایج نشان دادند که پس از 48 ساعت انکوباسیون، عصاره اتانولی در غلظت‌های 5 و mg/ml 10 تکثیر سلول‌های سرطانی MCF-7 را با تفاوت معنی‌دار با سلول‌های کنترل، کاهش داد (p
کلیدواژه‌ها
موضوعات
عنوان مقاله [English]
The Cytotoxic Effects of Ethanolic Extract of Leaf and Flower Buds of Crataegus melanocarpa on Human Breast (MCF-7) and Cervical (HeLa) Cancer Cell Line
نویسندگان [English]
- Mahmoud Alipour 1
- Farkhondeh Nemati 2
1 Department of Biology, The Biology teacher of Qaemshahr Education Office, Qaemshahr, IRAN
2 Assistant Professor in Animal Physiology, Department of Biology, Islamic Azad University, Qaemshahr Branch, qaemshahr, Iran
چکیده [English]
In the last few decades, research has been focused on the use of natural products for cancer therapy, such as crude plant extracts or some of different plant secondary metabolits. The objective of this study was to examine the invitro cytotoxic activities of crude ethanolic extract of leaf and flower buds of Crataegus melanocarpa, on human MCF-7 and HeLa cell lines, with different concentrations, by Dye exclusion and Micro culture tetrazolium test (MTT) assay. The optical density (OD) colored solution was quantified at 570 nm wavelengths by an ELISA Reader, after 24, 48 and 72 h incubation. The results showed that ethanolic extracts, after 48h incubation, at in 5 and 10 mg/ml suppressed the proliferation of cancerous MCF-7 cells by significant diffrence with control group (p
کلیدواژه‌ها [English]
- Cytotoxity
- Ethanol extract
- C. melanocarpa
- MCF-7 cell line
- HeLa cell line
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