نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناسی ارشد زیستشناسی سلولی و مولکولی، گروه زیستشناسی، دانشکده علوم پایه، دانشگاه حکیم سبزواری، سبزوار، ایران
2 دانشیار، گروه زیستشناسی، دانشکده علوم پایه، دانشگاه حکیم سبزواری، سبزوار، ایران
3 استاد، گروه زیستشناسی، دانشکده علوم، دانشگاه فردوسی مشهد، مشهد، ایران
چکیده
زمینه و هدف: هموستاز واکنش طبیعی بدن به آسیب به دلیل حفظ خون و عملکرد صحیح نداشتن این فرایند میتواند باعث ایجاد انعقاد خون خیلی کم (هموفیلی) شود. امروزه گیاهان دارویی به دلیل مقرونبهصرفه و کمخطربودن بیشتر مورد توجه قرار گرفتهاند. در طب سنتی ایران، از گل محمدی برای قطع خونریزی قاعدگی استفاده میشده است از اینرو هدف این پژوهش بررسی تأثیر عصاره هیدروالکلی گل محمدی بر پارامترهای انعقادی در شرایط In Vivo میباشد.
مواد و روشها: 56 موش نر سوری (5±25 گرمی) در 8 گروه (n=7) شامل سه گروه شاهد، شم، کنترل مثبت و پنج گروه آزمایشی شامل دوزهایmg/kg 500، 250، 100، 50، 25 عصاره هیدروالکلی گل محمدی به مدت 14 روز بهصورت تزریق درونصفاقی (i.p. ) تیمار شدند. برای ارزیابی نتایج، از آزمونهای BT، CT، PT، aPTT و شمارش پلاکت که آزمونهای غربالگری هموستازند استفاده شد.
یافتهها: آزمونهای BT، CT، PT و aPTT کاهش زمان خونریزی با افزایش غلظت عصاره را نشان داد بهطوری که در دوز mg/kg 500 اختلاف معناداری با گروه کنترل مشاهده شد (p<0.05)
نتیجهگیری: نظر به نتایج ترکیبات پلیفنولی مانند تاننها و گلیکوزیدهای موجود در گل محمدی نظیر ساپونینها در افزایش تجمع پلاکتی و کاهش زمان خونریزی و لختهشدن مؤثر بودهاند. شاید تأثیرگذاری گل محمدی بر هر دو مسیر بیرونی و داخلی انعقاد باشد که میتواند ناشی از حضور گالیک اسید، کافئیک اسید، کوئرستین و کامپفرول باشد.
تازه های تحقیق
https://scholar.google.com/citations?hl=fa&user=j4gAlqwAAAAJ
https://www.ncbi.nlm.nih.gov/myncbi/mitra.ebrahimi.1/bibliography/public
https://scholar.google.com/citations?user=az82V-4AAAAJ&hl=en
https://pubmed.ncbi.nlm.nih.gov/31637661/
https://scholar.google.com/citations?user=aCAyk7gAAAAJ
https://pubmed.ncbi.nlm.nih.gov/?term=fereidoni+M
کلیدواژهها
موضوعات
عنوان مقاله [English]
Investigating the Effect of Hydroalcoholic Extract of Rosa damascena Mill. on Coagulation Parameters in Male Mice
نویسندگان [English]
- Mitra Ebrahimi 1
- Jafar Vatandost 2
- Masoud Fereidoni 3
1 Master's degree in cellular and e molecular biology, Department of Biology, Faculty of Science Hakim Sabzevari University, Sabzevar, Iran
2 Assistant Professor, Department of Biology, Faculty of Science Hakim Sabzevari University, Sabzevar, Iran
3 Professor, Department of Biology, Faculty of Science , Ferdowsi University of Mashhad, Mashhad, Iran
چکیده [English]
Introduction: Homeostasis is the body's natural response to injury aimed at preserving blood volume; dysfunction in this process can lead to low blood coagulation, such as in hemophilia. Medicinal plants are gaining attention for their cost-effectiveness and low risk. In traditional Iranian medicine, Rosa damascena has been utilized to manage menstrual bleeding. This study aims to investigate the in vivo effects of hydroalcoholic extract of Rosa damascena on coagulation parameters.
Materials and Methods: 56 male Syrian mice (25 ± 5 g) were divided into eight groups (n=7), including control, sham, positive control, and five experimental groups. The mice were intraperitoneally treated with doses of 500, 250, 100, 50, and 25 mg/kg of hydroalcoholic extract of Rosa damascena for 14 consecutive days. Hemostasis screening tests, including bleeding time (BT), clotting time (CT), prothrombin time (PT), activated partial thromboplastin time (aPTT), and platelet count, were employed to evaluate the results.
Results: The BT, CT, PT, and aPTT tests indicated a decrease in bleeding time with increasing extract dosage, with a significant difference observed in the 500 mg/kg group compared to the control group (p < 0.05).
Conclusion: The findings suggest that polyphenolic compounds, such as tannins and glycosides, present in Rosa damascena, including saponins, effectively enhance platelet aggregation and reduce bleeding and clotting times. The impact of Rosa damascena on both external and internal coagulation pathways may be attributed to the presence of gallic acid, caffeic acid, quercetin, and kaempferol.
کلیدواژهها [English]
- Blood Coagulation
- Hemostasis
- Partial Thromboplastin Time
- Prothrombin Time
- Rosa
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