نوع مقاله : مقاله پژوهشی
نویسندگان
1 کارشناس ارشد، گروه زیستشناسی، دانشگاه پیام نور، ایران
2 دانشیار، گروه زیستشناسی، دانشگاه پیام نور، ایران
3 استادیار، گروه زیستشناسی، دانشگاه پیام نور، ایران
چکیده
زمینه و هدف: یکی از بهترین راهکارها برای حذف فلزات سمی، استفاده از باکتریهای مقاوم به این فلزات با فرایند پاکسازی زیستی میباشد. هدف از این پژوهش، جداسازی باکتریهای مقاوم به قلع، مس، کروم و نیکل از پسابهای صنعتی و شناسایی مولکولی آنها میباشد.
مواد و روشها: ابتدا از کارخانههای آبکاری واقع در استان تهران، پساب آلوده به فلزات سنگین جمعآوری گردید. نمونه پساب بر روی محیط LB Agar حاوی غلظتهای مشخص از فلزات سنگین کشت داده و باکتریهای رشدیافته جداسازی گردید. بر روی باکتریهای رشدیافته، حداقل غلظت مهارکننده رشد (MIC) فلزات سنگین با روش میکروبراث دایلوشن انجام شد. DNA ژنومی دوسویه با بالاترین میزان مقاومت، تخلیص و PCR با کمک پرایمرهای اختصاصی انجام شد. محصول PCR تعیین سکانس شد و ریبوتایپینگ انجام گردید.
یافته ها: از پساب حاوی فلزات سنگین، تعداد 9 باسیل گرم مثبت و منفی و کوکوباسیل گرم منفی جداسازی گردیدند. دوسویه باسیل گرم منفی، بیشترین مقاومت نسبت به فلزات سنگین را در آزمایش MIC از خود نشان دادند. این دوسویه بر اساس نتایج تعیین توالی، تحت عناوین انتروباکتر و سودوموناس شناسایی گردیدند.
نتیجهگیری: دوسویه با بالاترین مقاومت به چهار فلز از پساب کارخانجات آبکاری جدا شدند و به روش ارزیابی فیلوژنتیک مورد شناسایی مولکولی قرار گرفتند. میتوان از این باکتریها در تصفیه زیستی پسابهای حاوی فلزات سنگین استفاده کرد.
تازه های تحقیق
https://scholar.google.com/citations?view_op=new_profile&hl=en
https://pubmed.ncbi.nlm.nih.gov/?term=Mahta+Majdnia&sort=date
https://scholar.google.com/citations?user=xp2DXosAAAAJ&hl=en
https://pubmed.ncbi.nlm.nih.gov/?term=Maryam+Sadrnia&sort=date
https://scholar.google.com/citations?hl=en&user=GOPrr8oAAAAJ
https://pubmed.ncbi.nlm.nih.gov/?term=Fatemeh+Shahbazi&sort=date
https://pubmed.ncbi.nlm.nih.gov/?term=Nooshin+Sohrabi&sort=date
کلیدواژهها
موضوعات
عنوان مقاله [English]
Isolation of Heavy Metal Resistant Bacteria from Industrial Effluents and Molecular Ribotyping
نویسندگان [English]
- Mahta Majdnia 1
- Maryam Sadrnia 2
- Fatemeh Shahbazi 2
- Nooshin Sohrabi 3
1 Master In Sciences,, PayameNoor University, Iran
2 Associated Professor, Payame noor University, Iran
3 Assistant Professor, Department of Biology, PayameNoor University, Iran
چکیده [English]
Introduction: One of the best ways to remove toxic metals is to use bacteria resistant to these metals with a biological purification process. This research aims to isolate bacteria resistant to tin, copper, chromium, and nickel from industrial wastewater and their molecular identification.
Materials and Methods: wastewater contaminated with heavy metals was collected from electroplating factories located in Tehran province. The wastewater sample was cultured on LB Agar containing certain concentrations of heavy metals and the grown bacteria were isolated. In the grown bacteria, the minimum growth inhibitory concentration (MIC) of heavy metals was determined by the microbroth dilution method. The genomic DNA of two strains with the highest level of resistance, purity, and polymerase chain reaction was performed with the help of specific primers. The PCR product was sequenced and ribotyping was done.
Results: 9 gram-positive and negative bacilli and gram-negative coccobacilli were isolated from wastewater containing heavy metals. Two Gram-negative bacillus strains showed the highest resistance to heavy metals in the MIC test. Based on the sequencing results, these two strains were identified as Enterobacter and Pseudomonas.
Conclusion: Two strains with the highest resistance to four metals were isolated from the effluent of electroplating factories and phylogenetic evaluation was performed. These bacteria can be used in the biological treatment of wastewater containing heavy metals.
کلیدواژهها [English]
- heavy metals
- bacteria
- metal resistance
- molecular identification
- Paknia S, Sharif MAS, Heidarianpour A. The Effect of Resistance Training Along with Hawthorn Supplementation on Some Indices of Oxidative Stress in Alzheimer's Male Rats. male rats. Journal of Sport Biosciences. 2022; 14 (3): 81-94. https://doi.org/10.22059/jsb.2022.347813.1550
- Azevedo CV, Hashiguchi D, Campos HC, Figueiredo EV, Otaviano SFS, Penitente AR, et al. The effects of resistance exercise on cognitive function, amyloidogenesis, and neuroinflammation in Alzheimer’s disease. Frontiers in Neuroscience. 2023;17. https://doi.org/10.3389/fnins.2023.1131214
- Xue B, Waseem SMA, Zhu Z, Alshahrani MA, Nazam N, Anjum F, et al. Brain-Derived Neurotrophic Factor: A Connecting Link Between Nutrition, Lifestyle, and Alzheimer’s Disease. Frontiers in Neuroscience. 2022;16:925991. https://doi.org/10.3389/fnins.2022.925991
- Chortane OG, Hammami R, Amara S, Chortane SG, Suzuki K, Oliveira R, et al. Effects of multicomponent exercise training program on biochemical and motor functions in patients with Alzheimer’s dementia. Sustainability. 2022;14(7):4112. https://doi.org/10.3390/su14074112
- Zieneldien T, Kim J, Cao C. The multifaceted role of neuroprotective plants in Alzheimer’s Disease treatment. Geriatrics. 2022;7(2):24. https://doi.org/10.3390/geriatrics7020024
- Li D, Ma J, Wei B, Gao S, Lang Y, Wan X. Effectiveness and safety of ginkgo biloba preparations in the treatment of Alzheimer's disease: A systematic review and meta-analysis. Frontiers in Aging Neuroscience. 2023 https://doi.org/10.3389/fnagi.2023.1124710.
- Palimariciuc M, Balmus I-M, Gireadă B, Ciobica A, Chiriță R, Iordache A-C, et al. The Quest for Neurodegenerative Disease Treatment—Focusing on Alzheimer’s Disease Personalised Diets. Current Issues in Molecular Biology. 2023;45(2):1519-35 https://doi.org/10.3390/cimb45020098.
- Nasrolahi A, Javaherforooshzadeh F, Jafarzadeh-Gharehziaaddin M, Mahmoudi J, Asl KD, Shabani Z. Therapeutic potential of neurotrophic factors in Alzheimer’s Disease. Molecular Biology Reports. 2022:1-13. https://doi.org/10.1007/s11033-021-06968-9
- Gliwińska A, Czubilińska-Łada J, Więckiewicz G, Świętochowska E, Badeński A, Dworak M, et al. The Role of Brain-Derived Neurotrophic Factor (BDNF) in Diagnosis and Treatment of Epilepsy, Depression, Schizophrenia, Anorexia Nervosa and Alzheimer’s Disease as Highly Drug-Resistant Diseases: A Narrative Review. Brain Sciences. 2023;13(2):163. https://doi.org/10.3390/brainsci13020163
- Sadeghinejad M, Soltani Z, Afzalpour ME, Khaksari M, Pourranjbar M. What is the combined effect of intense intermittent exercise and Ginkgo biloba plant on the brain neurotrophic factors levels, and learning and memory in young rats? Pharmacological Reports. 2019;71(3):503-8 https://doi.org/10.1016/j.pharep.2019.02.006
- Eslimi Esfahani, D., Oryan, S., Hatami, M. Effect of Marshmallow extract on improving passive avoidance memory disorders in male Wistar rats. Journal of Animal Research (Iranian Journal of Biology), 2018; 31(1): 14-24. (Persian). DOI: 1001.1.23832614.1397.31.1.2.8
- Yaghoubi A. The Effects of Aerobic Training and Omega-3 Intake on Aβ42, Neprilysin, and γ-Secretase in the Hippocampus of Male Rats Alzheimer’s model. Tehran, Iran: Islamic Azad University. 2021. https://doi.org/10.21203/rs.3.rs-427829/v1
- Medhat E, Rashed L, Abdelgwad M, Aboulhoda BE, Khalifa MM, El-Din SS. Exercise enhances the effectiveness of vitamin D therapy in rats with Alzheimer’s disease: emphasis on oxidative stress and inflammation. Metabolic brain disease. 2020;35:111-20. https://doi.org/10.1007/s11011-019-00504-2
- Hasanvand B, Farhadi A. Effect of combined exercise and Ginkgo biloba supplementation for 8 weeks on brain-derived neurotrophic factor level in depressed older men. Iranian Journal of Ageing. 2021;16(2):234-47. (Persian) 32598/sija.16.2.2805.1
- Bakir F, ÇEVİK ÖS, KELOĞLAN SM, Şahin L. Effect of caffeine supplementation during treadmill exercise on hippocampal genes expression levels in adolescent rats. The European Research Journal. 2023;9(2):348-58 https://doi.org/10.18621/eurj.1241667.
- Afshani M, Nasiri E, Khalili M. The effect of sprint interval training with short repetitions on hippocampal Brain-derived neurotrophic factor levels, learning and spatial memory in adult Wistar rats. KAUMS Journal (FEYZ). 2023;27(1):761-9 .(Persian) DOI:48307/FMSJ.2023.27.1.12
- Shahidi S, Ghahremanitamadon F, AslS S, Komaki A, Afshar S, Hashemi-Firouzi N. Electrophysiological, behavioral and molecular study of vitamin E and Ginkgo biloba in a rat model of Alzheimer’s disease. Res J Pharmacogn. 2021;8(1):39-51 DOI: 10.22127/RJP.2020.250269.1630
- Mai G, Fan X. The Preventive and Therapeutic Prospects of Physical Activity on Alzheimer’s Disease and the Potential Underlying Mechanisms. 2023;5(2):1055
- Shamsipour S, Sharifi G, Taghian F. Impact of interval training with probiotic (L. plantarum/Bifidobacterium bifidum) on passive avoidance test, ChAT and BDNF in the hippocampus of rats with Alzheimer’s disease. Neuroscience letters. 2021;756:135949. https://doi.org/10.1016/j.neulet.2021.135949
- Campbell TS, Donoghue KM, Ghosh U, Nelson CM, Roth TL. Early life stress affects Bdnf regulation: a role for exercise interventions. International Journal of Molecular Sciences. 2022;23(19):11729. https://doi.org/10.3390/ijms231911729
- Vasconcelos-Filho FS, da Rocha-E-Silva RC, Martins JE, Godinho WD, da Costa VV, Ribeiro JK, et al. Neuroprotector effect of daily 8-minutes of high-intensity interval training in rat Aβ1-42 Alzheimer disease model. Current Alzheimer Research. 2020;17(14):1320-33. https://doi.org/10.2174/1567205018666210218161856