نوع مقاله : مقاله پژوهشی
نویسندگان
1 دانشجوی دکترای فیزیولوژی ورزش، گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، خرمآباد، ایران
2 دانشیار فیزیولوژی ورزش، گروه تربیت بدنی و علوم ورزشی، دانشکده ادبیات و علوم انسانی، دانشگاه لرستان، خرمآباد، ایران
3 استادیار فیزیولوژی ورزشی، دانشکده علوم انسانی و اجتماعی، دانشگاه اردکان، اردکان، ایران
4 دکترای فیزیولوژی ورزشی، دانشگاه فرهنگیان. دانشگاه فرهنگیان کرمانشاه، پردیس شهید صدوقی، کرمانشاه، ایران
چکیده
زمینه و هدف: ورزش یک گزینه غیردارویی امیدوارکننده برای بهتأخیرانداختن بالقوه شروع یا کندکردن پیشرفت آلزایمر است از اینرو این پژوهش درصدد یافتن مکانیسم احتمالی ارتباط عضله و هیپوکمپ تحت تأثیر تمرین ورزشی شنا در رتهای مبتلا به آلزایمر است.
مواد و روشها: 32 سر رت 6 هفتهای بهصورت تصادفی به چهار گروه شم (SH)، کنترل آلزایمری (AC)، تمرین (T) و تمرین آلزایمری (AT) تقسیم شدند. آلزایمر از طریق تزریق بتا آمیلوئید در هیپوکمپ القا شد. برنامه تمرین شامل 20 جلسه تمرین شنا با زمان فزاینده بود. پس از دوره تطبیقی، رتها از روز پنجم تا روز بیستم به مدت 30 دقیقه شنا کردند. پس از پایان مداخله، بافت عضله دوقلو و هیپوکمپ برداشته شد و با روش رنگآمیزی ایمونوهیستوفلروسنت بیان پروتئینهای موردنظر اندازهگیری شد. از آزمون همبستگی برای بررسی تغییرات شاخصهای موردمطالعه استفاده شد. سطح معنیداری 0/05 بود.
یافتهها: نتایج نشان دادند که بیان پروتئینهای مولکول چسبندگی سلول عصبی، سمافورین3A و پروفیلین ارتباط مثبت معنیدار با گیرنده استیلکولین نیکوتینی (0/001 P=) و ارتباط معکوس معنیدار با NLRP1 (0/05≥P) و Dead Cells (0/05≥P) داشتند.
نتیجهگیری: به نظر میرسد چون القای آلزایمر موجب تخریب پیوندگاه عصبی- عضلانی و نرونهای عصبی حرکتی میشود میتواند در افزایش التهاب هیپوکمپ مؤثر باشد از طرف مقابل تمرینات شنا مداخلهای مؤثر در راستای بهبود بازسازی آکسونی و شکلپذیری نورونی در نورونهای حرکتی میشود و از اینرو میتواند یک مداخله مؤثر در جهت پیشگیری و کنترل عوارض بیماری آلزایمر در سالمندی باشد.
تازه های تحقیق
https://scholar.google.com/citations?user=qtD1ZN0AAAAJ&hl=en&citsig=AM0yFClXG23KWiTu_K1QtQSF7Fpq
https://www.ncbi.nlm.nih.gov/myncbi/????.???????%20???????.2/bibliography/public/
https://scholar.google.com/citations?user=BtPgGnEAAAAJ&hl=en,
https://www.ncbi.nlm.nih.gov/myncbi/m.f.9/bibliography/public/
https://scholar.google.com/citations?view_op=list_works&hl=en&user=Nql7U1oAAAAJ
https://www.ncbi.nlm.nih.gov/myncbi/zahra.hemati%20farsani.1/bibliography/public/
https://scholar.google.com/citations?user=jZyl67sAAAAJ&hl=en
https://www.ncbi.nlm.nih.gov/myncbi/zeinab.gorgin%20karaji.1/bibliography/public/
کلیدواژهها
عنوان مقاله [English]
Investigating the Relationship between Neuromuscular Junction Preservation and Regeneration Factors and Hippocampal Inflammatory Factors under the Influence of Swimming Exercise in Alzheimer’s Model Rats
نویسندگان [English]
- Mohammad solimani farsani 1
- mohammad fathi 2
- zahra hemati 3
- zinab gorgin 4
1 PhD student, Dept. of Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran
2 Associate Prof. of Physical education, Dept. of Sport Sciences, Faculty of Human Sciences, Lorestan University, Khorramabad, Iran
3 Assistance Prof of Physical education, Department of Sport Sciences, Ardakan University, Ardakan, Iran
4 PhD of Physical education, Dept. of Physical education, Farhanguian University of Kermanshah Province, Kermanshah, Iran
چکیده [English]
Introduction: Exercise is a promising non-pharmacological option to potentially delay the onset or slow the progression of Alzheimer's disease. Therefore, this research seeks to find the possible mechanism of the connection between muscle and hippocampus under the influence of swimming exercise in rats with Alzheimer's disease. The significance level was 0.05.
Materials and Methods: Thirty-two 6-week-old rats were randomly divided into four groups: sham (SH), Alzheimer's control (AC), training (T) and Alzheimer's training (AT). Alzheimer's was induced by injecting beta-amyloid into the hippocampus. The training program consisted of 20 swimming training sessions with increasing time. After the end of the intervention, the tissue of the Gastrocnemius muscle and hippocampus was removed and the expression of the desired proteins was measured by immunohistofluorescent staining method. A correlation test was used to check the changes of the studied indicators.
Results: The results showed that the expression of neuron cell adhesion molecule, SEMA3A and profilins proteins had a significant positive relationship with NACHRa7 (P=0.001) and a significant inverse relationship with NLRP1 (P≥0.05) and Dead Cells (P≥0.05).
Conclusion: It seems that the induction of Alzheimer's causes the destruction of the neuromuscular junction and motor neurons. It can be effective in increasing hippocampus inflammation, on the other hand, swimming exercises is an effective intervention to improve axonal regeneration and neuron plasticity in motor neurons, and therefore it can be an effective intervention to prevent and control the complications of Alzheimer's disease in old age.
کلیدواژهها [English]
- Neuron Cell Adhesion Molecule
- Profilins
- Nicotinic Acetylcholine Receptor A7
- Dead Cells
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