نوع مقاله : مقاله پژوهشی
نویسندگان
1 استادیار، دکترای تخصصی علوم سلولی و مولکولی، گروه زیستشناسی، واحد تهران مرکزی، دانشگاه آزاد اسلامی، تهران، ایران
2 دانشیار، متخصص پوست و مو، گروه پژوهشی لیزر پزشکی، مرکز تحقیقات لیزر در پزشکی، پژوهشکده یارا، جهاد دانشگاهی واحد علوم پزشکی تهران، ایران
3 کارشناسی ارشد علوم سلولی و مولکولی، گروه پژوهشی لیزر پزشکی، مرکز تحقیقات لیزر در پزشکی، پژوهشکده یارا، جهاد دانشگاهی واحد علوم پزشکی تهران، ایران
چکیده
زمینه و هدف: استفاده از ترکیبات نانو بهطور فزاینده در حال رشد است. استفاده از ترکیبات نانو در فرایندهای پزشکی، کاربرد روزافزونی پیدا کرده است. یکی از جنبههای کاربردی فناوری نانو، استفاده از ترکیباتی نظیر هیدروژلها، نانولیپوزومها، نیوزومها و غیره بهعنوان یک سیستم تحویل دارو در درمان بیماری سرطان است .هدف از این مطالعه بررسی تأثیر ضدسرطانی نانوذرات نیوزومی حاوی دارو بر رده سلولی سرطانی سینه انسانی MCF-7 است.
مواد و روشها: ﺑﺮای ﺗﻬﯿﻪ ﻧﯿﻮزومهای کافئین از روش آبپوشانی لایه نازک اﺳﺘﻔﺎده ﺷﺪ. ﻣﻘﺎدﯾﺮ ﻣﺸﺨﺼﯽ از سورفکتانت، کافئین و ﮐﻠﺴﺘﺮول در اﺗـﺎﻧﻮل ﺣﻞ ﮔﺮدﯾﺪ و ﭘﺲ از ﺗﺒﺨﯿﺮ ﺑﺎ ﮐﻤﮏ روﺗـﺎری، در ﺑـﺎﻓﺮ فسفات حل گردید و سپس به کمک سونیکـاتور همگنسازی شد. قطر متوسط نیوزومهای کافئین با دستگاه زتاسایزر اندازهگیری شد. اثر سمیت سلولی با روش رنگسنجی 3، 4، 5 دیمتیل تیازول 2- ایل 2، 5 دیفنیل تترازولیوم ارزیابی شد.
یافتهها: تیمار سلولهای سرطانی سینه انسانی با غلظتهای مختلف از داروی نانو شده و داروی آزاد نشان داد که نانودارو توانایی مهار تکثیر سلولهای سرطانی را بیشتر از داروی آزاد دارد و غلظت 0/25 میلیگرم بر میلیلیتر از داروی نانونیوزوم تأثیر بیشتری بر کاهش سلولهای سرطانی داشته است.
نتیجهگیری: نانوذرات وزیکولی، سیستمهایی پیچیده با امتیازات و عیوب منحصربهفرد هستند که آنها را از سایر سیستمهای کلوئیدی مجزا میکند. ترکیبات وزیکولی بهعنوان اهداف جدید برای داروهای ضدسرطان در حال تولید میباشند.
کلیدواژهها
موضوعات
عنوان مقاله [English]
Study of Cytotoxic Effects of Caffeine- Loaded Niosomes on Human Breast Cancer Cells MCF-7
نویسندگان [English]
- Nakisa Zarrabi Ahrabi 1
- Seyed Mehdi Tabaie 2
- MARYAM JAHANSHIRI 3
1 Assistant Professor, Cellular and molecular sciences, Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran
2 Associate Professor, Dermatologist, Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
3 M.Sc, Cellular and molecular sciences, Department of Medical Laser, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran
چکیده [English]
Introduction: The use of nanoparticles in biomedical research have been increasingly developed in recent years. One of the applications of nanotechnology is the use of compounds such as hydrogels, micelles, nanoliposomes, nanofibers and niosomes as a delivery system to treat cancer. The aim of this study was to evaluate the ant antitumor effect of the caffeine-loaded niosomes on MCF-7 human breast cancer cell line.
Materials and Methods: The thin film hydrate on method was used to prepare caffeine-loaded niosomes. Certain amounts of surfactant, caffeine and cholesterol were dissolved in ethanol. Rotary evaporation was used to remove solvent from reaction mixture. Sample was dissolved in the phosphate buffer and homogenized with sonicator. The average diameters of caffeine-loaded niosomes were measured using a Zetasizer Nano system. Finally, the cytotoxicity effect was evaluated by MTT assay.
Results: The treatment of breast cancer cells with different concentrations of nano-sized drug and free drug showed that the nanoparticles had the ability to inhibit the MCF7 proliferation more than free drug. 0.25 mg/ml of niosomal drug had a greater effect on reducing of cancer cells viability.
Conclusion: Vesicular nanoparticles are complex systems with some advantages and disadvantages which sets these delivery systems apart from other colloid system. Vesicular nanoparticle is being developed as new drug delivery system for cancer treatment.
کلیدواژهها [English]
- "niosomes"
- " cytotoxic"
- " MTT"
- " caffeine
- " "Breast Cancer Cell Line MCF7"
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