The Effect of Progressive Resistance and Endurance Training on Gastrocnemius Muscle’s FNDC5 Gene Expression in Male Rats

Hoseinzadeh, Mohammad; Rashidlamir, Amir; Hejazi, Seyed Mahmud

Document Type : Original Article

Authors

¹ Department of physical education, Farhangian university, Tehran, Iran

² Department of physical education, Ferdowsi University of Mashhad, Mashhad, Iran

³ Department of physical education, Mashhad branch, Islamic Azad University, Mashhad, Iran

Abstract

Background and purpose: Fibronectin transmembrane type III domain-containing protein 5 (FNDC5) is a characteristic factor in distinguishing skeletal muscle cells. Since the productive effects of regular exercise are conveyed through the skeletal muscle, this study aims to compare the effects of progressive endurance and resistance training on FNDC5 gene expression in male rats’ gastrocnemius muscles.
Materials and Methods: 15 male rats were randomly assigned to three equal groups of: control, endurance training and resistance training. The endurance group performed 8 weeks of training on the treadmill. The resistance group did their exercises for eight weeks on a three-session-per-week basis on a one-meter-high ladder with 85○ slope. All subjects were anesthetized 72 hours after the experiment finished and the gastrocnemius removed. The level of FNDC5 expression was measured through real-time PCR method and one-way ANOVA applied to analyze the data. Level of significance was set to P≥0.05.
Results: One-way ANOVA showed a significant difference in FNDC5 expression among the groups (P=0.001, F=31.791). A subsequent Tukey test application showed FNDC5 gene expression of the gastrocnemius muscle was significantly higher in the resistance and endurance training groups than in the control group (P

Keywords

Main Subjects

Physical Education

References

[1] Panati K, Suneetha Y, Narala V.R. Irisin/FNDC5 – An updated review. European Review for Medical and Pharmacological Sciences. 2016; 20: 689-97.

[2] Khodadadi H, Rajabi H, Attarzadeh SR, Abbasian S. The Effect of High Intensity Interval Training (HIIT) and Pilates on Levels of Irisin and Insulin Resistance in Overweight Women. Iranian Journal of Endocrinology and Metabolism. 2014; 16 (3): 190-6. [in Persian]

[3] Aghamohammadi M, Habibi A, Ranjbar R. The effect of selective aerobic training on serum irisin levels and insulin resistance index in women with type 2 diabetes. Arak Medical University Journal (AMUJ). 2016; 18 (04): 1-9. [in Persian]

[4] Lui J, Cui X.Y, Yang Y.Q, Gao W, Sun L, Dong Y.C et al. Effects of high-intensity treadmill training on timeliness and plasticity expression of irisin in mice. EuropeanReviewforMedical andPharmacological Sciences. 2015; 19: 2168-273.

[5] Malekyian-Fini E, Kaviani-Nia A, Mahmoudi F. The interactive effect of aerobic training and resveratrol supplementation on C-reactive protein and metabolic profiles in women with type 2 diabetes. Journal of Kashan University of Medical Sciences. 2015; 19 (5): 372-81. [in Persian]

[6] Reisi J, Rajabi H, Ghaedi K, Marandi M, Asady samani Z, kazemi nasab F. Effect of eight weeks’ resistance training on plasma irisin protein level and muscle FNDC5 and adipose tissue UCP1 genes expression in male rats. Exercise physiology. 2015; (28): 118- 30. [in Persian]

[7] G. Swick A. Irisin, a novel myokine: potential role in obesity and diabetes. Heart Metab 2013; 61: 39-40.

[8] Barja-Fernández S, Folgueir C, Castelao C, Al-Massadi O, Bravo S.B, Garcia-Caballero T et al. FNDC5 is produced in the stomach and associated to body composition. Scientificreports. 2016; 6:23067: 1-12.

[9] Schumacher M, Chinnam N, Ohashi T, Sanjay Shah R, P. Erickson H. The Structure of Irisin Reveals a Novel Intersubunit-Sheet Fibronectin Type III (FNIII) Dimer. Journal of Biologycal Chemisty. 288 (47): 33738-44.

[10] Norheim F, Mikal Langleite T, Hjorth M, Holen T, Kielland A, Kristian Stadheim H et al. The effects of acute and chronic exercise on PGC-1a, irisin and browning of subcutaneous adipose tissue in humans 2014; 281: 739-49.

[11] Fain J,M. Company J,W. Booth C, Harold Laughlin M, Padilla J,T. Jenkins N et al. Exercise training does not increase muscle FNDC5 protein or mRNA expression in pigs. Metabolism. 2013; 62 (10): 1503- 11.

[12] Tsuchiya Y, Ando D, Goto K, Kiuchi M, Yamakita4 M, Koyama K. High-Intensity Exercise Causes Greater Irisin Response Compared with Low-Intensity Exercise under Similar Energy Consumption. Tohoku J. Exp. Med 2014; 233 (2): 135-40.

[13] Lecker S, Zavin A, Cao P, Arena R, Allsup K, M. Daniels K et al. Expression of the Irisin Precursor FNDC5 in Skeletal Muscle Correlates With Aerobic Exercise Performance in Patients With Heart Failure. Circ Heart Fail 2012; 812- 18.

[14] Miyamoto-Mikami E, Sato K, Kurihara T, Hasegawa N, Fujie S, Fujita S et al. Endurance Training-Induced Increase in Circulating Irisin Levels Is Associated with Reduction of Abdominal Visceral Fat in Middle-Aged and Older Adults. PLOS ONE 2015; 1-12.

[15] Boström P, Wu J,P. Jedrychowsk M, Korde A, Ye Li, C. Lo J et al. A PGC1α-dependent myokine that drives browning of white fat and thermogenesis. Nature 2012; 481(7382): 463–8.

[16] Disanzo B, You T. Effects of exercise training on indicators of adipose tissue angiogenesis and hypoxia in obese rats. Metabolism Clinical and Eeperimental 2014; 452-5.

[17] Matinhomaee H, Ziaolhagh S J, Azarbayjani M A, Piri M. Effects of Boldenone consumption and resistance exercise on hepatocyte morphologic damages in male wistar rats. European Journal of Experimental Biology. 2014; 4(2):211-4.

[18] Livak K J, Schmittgen T D. Analysis of Relative Gene Expression Data Using Real-Time Quantitative PCR and the 2^-ΔΔCt Method. Methods. 2001; 25: 402-8.

[19] Pukajło K, Kolackov K, Łaczmański Ł, Daroszewski J. Iryzyna – nowy mediator homeostazy energetycznej. Postepy Hig Med Dosw (online). 2015; 69: 233-42.

[20] Roca-Rivada A, Castelao C, L. Senin l, O. Landrove M, Baltar j, Crujeiras A B et al. FNDC5/Irisin Is Not Only a Myokine but Also an Adipokine PLOS ONE. 2013; 8(4): 1-10.

[21] Sanchis-Gomar F, Garatachea N, He Z, Pareja-Galeano H, Fuku N, Tian Y et al. FNDC5 (irisin) gene and exceptional longevity: a functional replication study with rs16835198 and rs726344 SNPs. Age. 2014; 36: 9733.

[22] Elsen M, Raschke S, Eckel J. Browning of white fat: does irisin play a role in humans?. Journal of Endocrinology. 2014; 222: 25– 38.

[23] Srivastava N. ATP binding cassette transporter A1 – key roles in cellular lipid transport and atherosclerosis. Molecular and Cellular Biochemistry. 2002; 237:155–64.

[24] Lally J.S, Ford R.J, Johar J, Crane J.D, Kemp B.E, Steinberg G.R. Skeletal muscle AMPK is essential for the maintenance of FNDC5 expression. Physiological Reports. 2015; 3 (5): 12343-52.

[25] Pekkala S, Wiklund P, Hulmi J, Ahtiainen J, Horttanainen M, Pollanen E, et al. Are skeletal muscleFNDC5gene expression and irisin release regulated by exercise and related to health?. J Physiol. 2013; 591 (21): 5393-400.

[26] Ghanbari-Niaki A, Ghanbari-Abarghooi S, Rahbarizadeh F, Zare-Kookandeh N, Gholizadeh M, Roudbari F et al. Heart ABCA1 and PPAR-α Genes Expression Responses in Male rats: Effects of High Intensity Treadmill Running Training and Aqueous Extraction of Black Crataegus-Pentaegyna. Research in Cardiovascular Medicine. 2013; 1 (5): 153-59.

Journal of Sabzevar University of Medical Sciences

The Effect of Progressive Resistance and Endurance Training on Gastrocnemius Muscle’s FNDC5 Gene Expression in Male Rats

References

References

Volume 25, Issue 5
November and December 2018
Pages 629-637

The Effect of Progressive Resistance and Endurance Training on Gastrocnemius Muscle’s FNDC5 Gene Expression in Male Rats

References

References

Volume 25, Issue 5November and December 2018Pages 629-637

Volume 25, Issue 5
November and December 2018
Pages 629-637