Detection of Quinolone resistance genes in the Salmonella enteritidis strains isolated from food samples by Multiplex-PCR method

Abdi, Hanieh; Amini, Kumarss; Tabatabaiee Bafroie, Akram Sadat

Document Type : Original Article

Authors

Abstract

Introduction: Antibiotic resistance in Salmonella is considered in the past two decades due to resistance associated with the consumption of drugs as additives in animal food chain, indiscriminate use of arbitrary know the people and the administration. Mortality in epidemics caused by strains resistant to antibiotics has increased. The aim of this study was to investigate the quinolone resistance genes in Salmonella enteritidis isolated from food.
Materials and Methods: The study included 60 isolates of S. Enteritidis isolates from food-microbiological research laboratory of microbial collection Pasargadae was taken. Antibiotic resistance patterns of Salmonella isolates to disk antibiotics (amoxicillin, nitrofurantoin, nalidixic acid, ciprofloxacin, cephalothin, norfloxacin and ofloxacin) with disk diffusion testing by Kirby - Bauer was based on CLSI. The genes qnrB, qnrS and qnrA simultaneously with Multiplex PCR method was investigated.
Results: The results show that all 60 isolates tested (100%) were sensitive to cephalothin complete and while most resistance to nitrofurantoin among the isolates (50 isolates, 83. 3%) and then nalidixic acid ( 44 isolates, 73.3%) is. A total of 8 isolates of Salmonella enteritidis was identified 5 qnr B gene (62.5%) and 3 isolates qnr S gene (37.5%), respectively.
Conclusion: the qnrS and qnrB resistance genes are play an important role in the creation and transmission of antibiotic resistance. Screening quinolone resistance gene as a marker and mark the acquisition and development of antibiotic resistance can be used as an important strategy in the fight against antibiotic resistance in bacteria.

Keywords

Main Subjects

Microbiology

References

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Journal of Sabzevar University of Medical Sciences

Detection of Quinolone resistance genes in the Salmonella enteritidis strains isolated from food samples by Multiplex-PCR method

References

References

Volume 25, Issue 2
May and June 2018
Pages 169-175

Detection of Quinolone resistance genes in the Salmonella enteritidis strains isolated from food samples by Multiplex-PCR method

References

References

Volume 25, Issue 2May and June 2018Pages 169-175

Volume 25, Issue 2
May and June 2018
Pages 169-175