Anatomy, Histologyو Embryology
elham hoveizi; Kiavash Hushmandi
Volume 29, Issue 3 , September and October 2022, , Pages 330-343
Abstract
Introduction: In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and ...
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Introduction: In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and A549 cancer cells.
Materials and Methods: In this experimental study, the stoke of coumarin was prepared and then for 1,3, and 5 days at concentrations of5, 10, 15, 20, and 25 μM the cells were treated and evaluated on viability days and morphology of the cells indefinite days. The IC50 concentration of coumarin was calculated using MTT assay in two cell lines. Also, the expression of the involved genes in apoptosis such as Bax, Bad, and Bcl-2 was evaluated by the qRT_PCR method. Data were analyzed by a one-way ANOVA test.
Results: The results showed that coumarin reduced the viability and proliferation of HT-29 and special A549 cells by dose and time significantly (P≤0.001), as well as the viability rate of cells in treated cells on the fifth day, significantly decreased compared to the control group (P <0.05). Morphological changes such as reduced chromatin density, cell turnover were also noticeably observed in the cells. Also, molecular results showed that coumarin could significantly increase the expression of Bax, Bad genes and decrease the expression of Bcl-2 gene expression. That these genetic changes in A549 cells were significantly greater than HT-29.
Conclusion: Coumarin is capable of anti-proliferative activity and induces apoptosis effectively against colon and lung cancer cells.
Physical Education
soudabeh rezaei; Neda Khaledi
Volume 28, Issue 5 , November and December 2021, , Pages 688-699
Abstract
Introduction: Increasing the expression of FOXO1 gen in hippocampus as well as inflammatory factor of TNF-α in diabetic individual's causes the expression of genes involved in apoptosis and disrupts its performance. The aim of the present study was investigation of the effect of 6-weeks HIIT on ...
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Introduction: Increasing the expression of FOXO1 gen in hippocampus as well as inflammatory factor of TNF-α in diabetic individual's causes the expression of genes involved in apoptosis and disrupts its performance. The aim of the present study was investigation of the effect of 6-weeks HIIT on FOXO1 gene expression in the hippocampus and level of serum TNF-α in male Wistar diabetic rats.Materials and Methods: For this study, 48 male Wistar rats (4 weeks old) with 150±10 g weight were categorized in 4 groups of (n=12): diabetic rats, exercise diabetic, control and exercise control. For induction of diabetes, peritoneal injection of STZ solution (50 mg/kg) was used. After a week of familiarization with the environment and practice, HIIT protocol consisted of 3 days per week for 6 weeks with 50% to 110% of maximum oxygen consumption was performed. 24 hours after the completion of the exercise, the functional test was taken and the animals were autopsy 48 hours after the functional test. FOXO1 gene expression was evaluated using Real Time PCR technique.Results: Regarding the results, HIIT significantly decreased FOXO1 gene expression in training groups (P≤0.01). Also, TNF-α protein decreased significantly (P≤0/05). Weight gain of the hippocampus was associated with a reduction in the FOXO1 gene in the diabetic training group.Conclusion: HIIT probably reduce the weight loss of the hippocampal tissue that caused by diabetes, by reducing the FOXO1 apoptotic and inflammatory factors of TNF-α.
Physical Education
mehdi yadegari; Simin Riahy; Shadmehr Mirdar; Gholamreza Hamidian
Volume 26, Issue 3 , September and October 2019, , Pages 393-402
Abstract
Background: This study investigated the effect of 3-weeks Taper in hypoxic environment on the ratio of Bax / Bcl2 and alveolar epithelial cells populations in the lung.Materials and Methods: Thirty-five rats (4 weeks old, weighing 8 ± 72 g) were divided in five groups: control (6 weeks), training ...
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Background: This study investigated the effect of 3-weeks Taper in hypoxic environment on the ratio of Bax / Bcl2 and alveolar epithelial cells populations in the lung.Materials and Methods: Thirty-five rats (4 weeks old, weighing 8 ± 72 g) were divided in five groups: control (6 weeks), training (6 weeks), hypoxia (3 weeks), hypoxia - taper (3 weeks) and control (9 weeks). Samples entered to the hypoxia after six weeks of interval training and lived there for 3 weeks. another group with exposure to hypoxic environment , implementation Taper technique.Bax/Bcl2 ratio of lung measured by immunohistochemistry and type II and type I pneumocytes population of pulmonary alveolar measured with stereologically.Results: Bax/Bcl2 ratio and type II pneumocytes population Increased and type I pneumocytes population Decreased After six weeks high intensity interval training (P≤0.5).Bax/Bcl2 ratio and type I pneumocytes population of hypoxia group decreased compared with training group (P≤0.5). three weeks taper in hypoxia group, Led to decrease of Bax/Bcl2 ratio , type II population pneumocytes and Increase of type I pneumocytes (P≤0.5).Conclusion: It seems high intensity interval training and subsequent hypoxia can increase pulmonary alveolar apoptosis. also appears taper is a Efficient Method to decrease of apoptosis lung.
Physical Education
Ghader Rahimzadeh; Mohammadali Azarbaijani; Hassan Matin homaei
Volume 26, Issue 2 , July and August 2019, , Pages 161-170
Abstract
AbstractBackground: Today, the use of supplements and herbal extracts has been widely used to prevent exercise induced apoptosis and improve exercise training adaptations. Thus, the present study examined the effect of the 12 weeks aerobic training with Origanum vulgare ethanolic Extract supplementation ...
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AbstractBackground: Today, the use of supplements and herbal extracts has been widely used to prevent exercise induced apoptosis and improve exercise training adaptations. Thus, the present study examined the effect of the 12 weeks aerobic training with Origanum vulgare ethanolic Extract supplementation on the myocardial apoptosis in young male rats.Methods: In this experimental study,40 male Wistar rats (8 weeks old)with an average(weight 129±17.86 in gr)randomly divided in five equal groups:basic(n=8),control(n=8),Origanum vulgare(n=8), aerobic training(n=8)and aerobic training-Origanum vulgare(n=8).The aerobic training and aerobic training - Origanum vulgare groups participated in a 12-week program (5 sessions of 10-60 minutes each week, with an intensity of 75-80% of maximum oxygen consumption) running on animal treadmill (15% incline and 24-33 m/s).Also,Origanum vulgare Ethanolic extract was used for supplementation.Forty-eight hours after the last exercise session,a part of the left venticular tissue of the heart was removed and expression of Bax,Bcl2 and caspase-9 proteins was investigated using RT-PCR method. Data were analyzed byTwo-way ANOVA test (P
Susan Gordehie; Reza Sheikhnejad; Seyed Abdolhamid Angaji; Mahmoud Khanaki; Vahid Marandi
Volume 25, Issue 1 , May and June 2018, , Pages 60-68
Abstract
Background and Purpose: Pancreatic cancer is one of the most leading causes of cancer-related deaths. Considering the role of Bcl-2 family gene in apoptosis that are known to be over-expressed in most cancers, this study focused on the detection of Bcl-2 translocation t(14;18) to the immunoglobulin heavy ...
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Background and Purpose: Pancreatic cancer is one of the most leading causes of cancer-related deaths. Considering the role of Bcl-2 family gene in apoptosis that are known to be over-expressed in most cancers, this study focused on the detection of Bcl-2 translocation t(14;18) to the immunoglobulin heavy chain (IgH) that may contribute to the pathogenesis of pancreatic cancer. Materials and Methods: Forty-nine samples of paraffin embedded tissues (extracted from 1537 slides of 105 patients in one of the major local cancer centers) were investigated for detection of Bcl-2 translocation t(14;18) by standard PCR. Results: The Bcl-2 t(14;18) translocation was detected in 23 of all 49 patients (46.9%), including 10 of 23 translocated patients (43%) with break points within the mbr cluster, 11 with involvement of the mcr locus (48%), and 2 in the icr locus (9%). Discussion: Detection of Bcl2 translocation in pancreatic cancer is in agreement with results from other studies, and shows this rearrangement could be considered as a new treatment strategy based on apoptosis or personalized treatment. Keywords: apoptosis, Bcl-2 translocation, pancreatic cancer,paraffin embedded tissue, personalized treatment, polymerase chain reaction.
Sayedeh Elmira Yazdi-Rouholamini; Nasrin Motamed; Mohammad Tahmasb; Kobra Omidfar
Volume 23, Issue 3 , July and August 2016, , Pages 386-391
Abstract
Background & Objectives: Metastatic cancers, such as breast cancer, are generally resistant to chemotherapy. Therefore many researches try to find new and effective compounds to treat this type of cancer. In this study, we examined the Silibinin toxic effect on tumor growth and proliferation of MCF-7 ...
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Background & Objectives: Metastatic cancers, such as breast cancer, are generally resistant to chemotherapy. Therefore many researches try to find new and effective compounds to treat this type of cancer. In this study, we examined the Silibinin toxic effect on tumor growth and proliferation of MCF-7 cell line. Materials & methods MTT assay was used to assess the inhibitory effect of silibinin on metabolic activity of MCF_7 cells. For this purpose, seeded cells were plated on to 96-well plates and then exposed to varying concentrations of silibinin (0-350 ug/mL) for different time (24, 48 and 72 h). The percentage of metabolic activity was calculated according to the MTT test. In next step, in order to evaluate the effect of silibinin on apoptosis in MCF_7 cells, Caspase 3/7 test was used. Results: Our results indicate that silibinin inhibited metabolic activity of MCF-7cells in a dose-dependent manner and it can induce apoptosis pathway in these cells. Conclusion: The study showed that treatment of MCF-7 cell line with Silibinin can be through induction of apoptosis, inhabitation of the growth and proliferation of cells. Due to the significant inhibitory effect of silibinin on MCF-7 cells, it seems important for researchers to utilize milk thistle in the treatment and prevention of cancer.
Tahmineh Peirouvi; Shiva Roshan Milani; Siamak Salami; Mohammad Ghaderi; Alireza Shams
Volume 21, Issue 3 , July and August 2014, , Pages 461-476
Abstract
Background: Gonadotropin-releasing hormone (GnRH) has been found to be expressed in ovaries in addition to hypothalamus to modulate cell differentiation and induces atretic follicles. Since the death of granulosa cells during the process of follicular atresia occurs by apoptosis phenomenon, in this study, ...
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Background: Gonadotropin-releasing hormone (GnRH) has been found to be expressed in ovaries in addition to hypothalamus to modulate cell differentiation and induces atretic follicles. Since the death of granulosa cells during the process of follicular atresia occurs by apoptosis phenomenon, in this study, we investigated the occurrence of apoptosis of granulosa cells of rat ovarian follicles under the influence of Buserelin acetate, a GnRH agonist.
Materials and Methods: In this experimental case-control study, twelve 25-day-old female Wistar rats were randomly divided into 2 groups. The study and control groups received 0.2 mg/kg/d Buserelin acetate and normal saline, respectively, for 4 days. 24 hours after the last injection, the rats were anesthetized with a lethal dose of chloroform and ovaries were removed. Specimens were fixed in 10% formalin. After the passage, five-micron sections were prepared using a rotary microtome. Measurement of apoptosis was performed using a calibrated light microscope after TUNEL POD staining. Data were analyzed in GraphPad InStat software using independent t-test. P ≤ 0/05 was statistically considered significant.
Results: These data showed the average percentage of apoptotic cells in the control group 2/14 ± 0/52, and in the experimental group 3/75 ± 1/71. This difference was statistically significant (p≤0.0001).
Conclusion: These findings suggest that Buserelin acetate increases apoptosis in the granulosa cells of ovarian follicles.
MB ARDESHIR LARIJANI; H RAMIN; A LASHKARI; P SHOUSHTARIZADEH; V HAGHPANAH; SM TAVANGAR
Volume 14, Issue 1 , March and April 2007, , Pages 32-37
Abstract
Background and Purpose: Surviving is one of the eight members of the human inhibitors of apoptosis (IAP), which appears to be differentially expressed in cancer cells. This study was conducted to evaluate the clinical usefulness of survivin immunostaining to distinguish between follicular adenoma and ...
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Background and Purpose: Surviving is one of the eight members of the human inhibitors of apoptosis (IAP), which appears to be differentially expressed in cancer cells. This study was conducted to evaluate the clinical usefulness of survivin immunostaining to distinguish between follicular adenoma and carcinoma of thyroid.
Methods and Materials: This study was a cross-sectional descriptive-analytical research. The study population was tissue samples from surgery patients at Shariati Hospital in Tehran, Iran. All samples were studied through immunohistochemical staining for surviving.
Results: Surviving expression was significantly (p