Mryam Salehi; Mehvash Jafari; Alireza Asgari
Volume 21, Issue 6 , January and February 2015, , Pages 1081-1089
Abstract
Background: Diazinon (DZN) is one of the most widely used organophosphates in agriculture. Vitamins C and E are antioxidants protecting cells from oxidative stress. The aim of this study was to evaluate the protective role of vitamins E and C as antioxidant in reduction of DZN-induced oxidative stress ...
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Background: Diazinon (DZN) is one of the most widely used organophosphates in agriculture. Vitamins C and E are antioxidants protecting cells from oxidative stress. The aim of this study was to evaluate the protective role of vitamins E and C as antioxidant in reduction of DZN-induced oxidative stress in rat liver.
Materials and Methods: In present experimental study, male Wistar rats were randomly divided into six groups including control group (corn oil as DZN solvent), DZN group (100 mg/kg), vitamin E group (150 mg/kg), vitamin C group (200 mg/kg), vitamin E+DZN group and vitamin C+DZN group which were given intraperitoneally. Animals were anesthetized by ether 24 hours after injectionand liver tissues were quickly removed. After tissues homogenization, superoxide dismutase (SOD), catalase (CAT), glutathione S-transferase (GST) and lactate dehydrogenase (LDH) activities, as well as glutathione (GSH) and malondialdehyde (MDA) levels were determined by biochemical methods. The data were statistically analysed using analysis of variance (ANOVA) followed by post hoc analysis using Tukey test.
Results: DZN increased SOD, CAT, LDH and GST activities and MDA level and decreased GSH content in liver. Administration of vitamins E and C inhibited the changed in these parameters.
Conclusion: Vitamins E and C as antioxidant decrease DZN-induced oxidative stress in rat liver by scavenging free radicals but they does not protect completely.
Fatemeh RahimiAnbarkeh; Mohammadreza Nikravesh; Mehdi Jalali; Hamidreza Sadeghnia; Zinat Sargazi
Volume 21, Issue 4 , September and October 2014, , Pages 550-558
Abstract
Background: Diazinon (DZN) is an organophosphate (OP) that inhibit cholinesterase activity and induce oxidative stress in the target tissues such as the the reproductive system .The aim of present study was to evaluate the effects of diazinon on cholinesterase activity in blood serum and erythrocytes ...
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Background: Diazinon (DZN) is an organophosphate (OP) that inhibit cholinesterase activity and induce oxidative stress in the target tissues such as the the reproductive system .The aim of present study was to evaluate the effects of diazinon on cholinesterase activity in blood serum and erythrocytes of male rats and to assess the protective role of vitamin E.
Material and Methods: In this experimental study, thirty adult male wistar rats (200 - 250 gr) were divided into 5 groups (n = 6): control group (did not receive any material), sham group (received only pure olive oil), experimental group 1 (DZN, 60 mg/kg), experimental group 2 (received DZN+Vit E daily, with the same dose) and experimental group 3(Vit E 200 mg/kg). DZN and solvent was injected intraperitoneally (IP) and vitamin E was administrated by gavage. After 6 weeks, 3 ml blood from the heart tissue was taken and titrimetric and Ellman’s method respectively was used for serum and erythrocyte cholinesterases activity evaluation. Data were analyzed using SPSS software. P