The Effect of glial cells inhibition on the progression of seizures induced by chemical kindling in male rats

Tavasoli, Zohreh; Hosseinmardi, Narges; Janahmadi, Mahyar; Golpayegani, Mehdi; Salari, Farhad; Jafarzadeh, Delaram

Document Type : Original Article

Authors

¹ Ph.D. Student, Physiology Department and Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

² Associate Professor, Physiology Department and Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

³ Professor, Physiology Department and Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

⁴ Medicine Student, Physiology Department and Neuroscience Research Center, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran

Abstract

Background & Objectives: Considering the role of glial cells in synaptic transmission, regulation of neurotransmitter concentration in synaptic cleft, K⁺ buffering, and releasing the gliotransmitters, the purpose of this study is to investigate the effect of glial cells inhibition on the progression of seizures induced by chemical kindling in rats.
Materials & Methods: In chemical kindling, animals received Pentylenetetrazol, 35 mg/kg each 48 hours, intraperitoneally and five different stages of seizure were appeared gradually and seizure parameters including maximum seizure stage (SS), stage 4 latency (S4L), stage 4&5 duration (S5D), and seizure duration (SD) were measured during 20 min after PTZ injection. Then seizure parameters were evaluated in animals treated with intracerebroventricular (icv) administration of Fluorocitrate (as a glial cells inhibitor), injected 30 min before PTZ, and compared with PTZ treated animals.
Results: Results showed that glial cells inhibition with ICV injection of Fluorocitrate decreased SS, S5D, and SD and increased S4L significantly (P<0.05, P<0.01, P<0.001).

Conclusion: On the basis of obtained results, it may be concluded that glial cells inhibition reduces spreading rate of epileptiform activity in the nervous system, and the duration of neuronal hyperexcitability and, also, prevents the progression of seizure to final stages.

Keywords

Main Subjects

Physiology & Pharmacology

References

[1] White HS. Comparative anticonvulsant and mechanistic profile of the established and newer antiepileptic drugs. Epilepsia, 1999; 40(5): 2-10.

[2] Fathollahi Y, Motamedi F, Semnanian S, Zardoshti M. Repeated administration of pentylenetetrazol alters susceptibility of rat hippocampus to primed-burst stimulation: evidence from in vitro study on CA1 of hippocampal slices. Brain Research, 1996; 738(1): 138-41.

[3] Harding AJ, Das A, Kril JJ, Brooks WS, Duffy D, Halliday GM. Identification of families with cortical Lewy body disease. American Journal of Medical Genetics Part B: Neuropsychiatric Genetics, 2004; 128(1): 118-22.

[4] Natelson S, Miletich DJ, Seals CF, Visintine DJ, Albrecht RF. Clinical biochemistry of epilepsy. I. Nature of the disease and a review of the chemical findings in epilepsy. Clinical Chemistry, 1979; 25(6): 889-97.

[5] Wetherington J, Serrano G, Dingledine R. Astrocytes in the epileptic brain. Neuron, 2008; 58(2): 168-78.

[6] Todd KJ, Serrano A, Lacaille JC, Robitaille R. Glial cells in synaptic plasticity. Journal of Physiology, 2006; 99(2): 75-83.

[7] Clasadonte J, Dong J, Hines DJ, Haydon PG. Astrocyte control of synaptic NMDA receptors contributes to the progressive development of temporal lobe epilepsy. Proceedings of the National Academy of Sciences of the United States America, 2013; 110(43): 17540-5.

[8] Allen NJ, Barres BA. Neuroscience: glia—more than just brain glue. Nature, 2009; 457(7230): 675-7.

[9] Volterra A, Steinhäuser C. Glial modulation of synaptic transmission in the hippocampus. Glia, 2004; 47(3): 249-57.

[10] Amiri M, Bahrami F, Janahmadi M. Functional contributions of astrocytes in synchronization of a neuronal network model. Journal of Theoretical Biology, 2012; 292: 60-70.

[11] Seifert G, Carmignoto G, Steinhäuser C. Astrocyte dysfunction in epilepsy. Brain Research Reviews, 2010; 63(1): 212-21.

[12] Palizvan MR, Fathollahi Y, Semnanian S, Hajezadeh S, Mirnajafizadh J. Differential effects of pentylenetetrazol-kindling on long-term potentiation of population excitatory postsynaptic potentials and population spikes in the CA1 region of rat hippocampus. Brain Research, 2001; 898(1): 82-90.

13. Hansen SL, Sperling BB, Sánchez C. Anticonvulsant and antiepileptogenic effects of GABA A receptor ligands in pentylenetetrazole-kindled mice. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 2004; 28(1): 105-13.

[14] Hassel B, Paulsen RE, Johnsen A, Fonnum F. Selective inhibition of glial cell metabolism in vivo by fluorocitrate. Brain Res, 1992; 576(1): 120-4.

[15] Lorke D. A new approach to practical acute toxicity testing. Archives of Toxicology, 1983; 54(4): 275-87.

[16] Kandel ER, Schwartz JH, Jessell TM, editors. Principles of neural science. New York: McGraw-Hill, 2000.

[17] Parpura V, Heneka MT, Montana V, Oliet SH, Schousboe A, Haydon PG, Stout RF, Spray DC, Reichenbach A, Pannicke T, Pekny M. Glial cells in (patho) physiology. Journal of Neurochemistry, 2012; 121(1): 4-27.

[18] Scharfman HE, Binder DK. Aquaporin-4 water channels and synaptic plasticity in the hippocampus. Neurochemistry International, 2013; 63(7): 702-11.

[19] Hsu MS, Seldin M, Lee DJ, Seifert G, Steinhäuser C, Binder DK. Laminar-specific and developmental expression of aquaporin-4 in the mouse hippocampus. Neuroscience, 2011; 178: 21-32.

[20] Binder DK, Yao X, Verkman AS, Manley GT. Increased seizure duration in mice lacking aquaporin-4 water channels. Acta Neurochirurgica Supplement, 2006; 96: 389-92.

[21] Araque A, Parpura V, Sanzgiri RP, Haydon PG. Tripartite synapses: glia, the unacknowledged partner. Trends in Neurosciences, 1999; 22(5): 208-15.

Journal of Sabzevar University of Medical Sciences

The Effect of glial cells inhibition on the progression of seizures induced by chemical kindling in male rats

References

References

Volume 24, Issue 1
April & May
March and April 2017
Pages 71-77

The Effect of glial cells inhibition on the progression of seizures induced by chemical kindling in male rats

References

References

Volume 24, Issue 1April & MayMarch and April 2017Pages 71-77

Volume 24, Issue 1
April & May
March and April 2017
Pages 71-77