Authors
Abstract
Background and Purpose: P53 gene is one of the genes which undergoes mutations in breast cancer. The rate of p53 gene mutation has been variously reported. However, its mutation is considered resistant to chemotherapy. This study is intended to determine p53 gene overexpression with histopathological parameters in breast cancer. Methods and Materials: This descriptive analytical research was conducted on 80 samples of non-randomized breast cancer cases admitted to hospitals in Sabzevar, Iran from 2007 to 2010. After samples were fixed in formalin, they underwent tissue processing and were stained by H&E. After malignancy diagnosis, the p53 gene overexpression was detected by immunohistochemical methods in the samples after fixation, tissue processing and antigen retrieval. The obtained data were analyzed in SPSS 11.5 using chi-square test. Results: Out of 80 specimens of breast cancer, p53 gene expression was observed in 39 specimens (48.8%), which was one positive (+) in 21 cases, two positive (++) in 10 cases and three positive (+++) in 8 cases. No significant relationships were observed between p53 protein stability with tumor stages, as well as age groups of over 45 years and below with tumor stages. Conclusion: P53 protein stability was detected in about half of the samples, which can signify the high rate of mutation of p53 gene in breast cancer in Sabzevar, Iran.
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