Authors
- Somayyeh Mohammadi
- Majid Esmaeelizad
- Abdolhamid Angji
- Mohammad Tahmasb
- Roghayeh Mesri
- Mojgan Ahmadzadeh
Abstract
Background and purpose: Hydatidosis is a parasitic disease that transmits by Echinococcusgranulosus eggs excreted from the feces of infected dogs to humans and animals. This parasite is one of the tapeworms of the family Taeniidae which causes widespread health problems among humans and also economic loss in animal. Therefore, designing a new generation vaccine is important to prevent the disease.
Materials and methods: In this study, the linear T-cell epitopes were selected. The coding sequence of epitopes was synthesized and cloned to pEGFP-N1vector. The recombinant vector was transfected into CHO cells by genpulser. Expression of fusion Protein was confirmed by fluorescence microscopy.
Results: The accuracy of cloning was confirmed by both colony PCR on the clone and the subsequent sequencing. Fluorescence microscopy showed fusion protein was expressed in CHO.
Conclusion: The results showed that, linear T-cell epitope of EG95 antigen was cloned correctly in pEGFP vector and its expression confirmed that this recombinant vector can be used to construct a DNA vaccine model against the Echinococcusgranulosus.
Keywords
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