Document Type : Original Article
Authors
- Mina Abroudi 1
- Ghazaleh Dadashizadeh 2
- Saeideh sadat Shobeiri 3
- Azar Hosseini 4
- Omid Gholami 5
- Reza Ataee Disfani 6
- Amir Raoofi 7
- Davood Mahdian 8
- Maryam Khosrojerdi 9
1 Master of Medical Biology, Cell and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
2 Master of Hematology, Cell and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
3 PhD in Immunology, Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
4 Pharmacology Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
5 Associate Professor, Department of Pharmacology, Faculty of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran
6 medical students, student research committee, Sabzevar University of Medical Sciences
7 Assistant Professor, Department of Anatomical Sciences, Cell and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
8 Cellular and Molecular Research Center, Department of Pharmacology, Sabzevar University of Medical Sciences, Sabzevar, Iran
9 Master of Nursing, Vice President of Research and Technology, Sabzevar University of Medical Sciences, Sabzevar, Iran
Abstract
Introduction: Hesperidin, a flavonoid found in the citrus family, has been evaluated for its anti-cancer effects on various cell lines, including KYSE30, PC12, SW48, and DU14.
Materials and Methods: The cells were treated with various concentrations of hesperidin (0.75–100 µM) for 24, 48, or 72 hours, and cell viability was assessed using the MTT assay. The expression of genes, including Bax, Bcl-2, and Caspase-3, was measured by qRT-PCR. Apoptosis was determined using propidium iodide staining followed by flow cytometry.
Results: The results demonstrated that hesperidin inhibited the proliferation of cells with varying potencies. Propidium iodide staining revealed that hesperidin increased the number of apoptotic cells, which was confirmed by the measurement of gene expression. Hesperidin decreased the expression of Bcl-2 while increasing the expression of Bax and Caspase-3.
Conclusion: We conclude that hesperidin, as a natural product, may be used in the treatment of various human cancers in combination with chemical drugs.
Keywords
Main Subjects
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