Document Type : Original Article
Authors
- Mina Abroudi 1
- Ghazaleh Dadashizadeh 2
- saeideh sadat shobeiri 3
- Azar Hosseini 4
- omid gholami 5
- Reza Ataee Disfani 6
- Davood Mahdian 7
- MARYAM KHOSROJERDI 8
1 Department of Biochemistry, Fars Science and Research branch, Islamic Azad University, Fars, Iran
2 Cellular and Molecular Research Center, Sabzevar University of Medical Sciences, Sabzevar, Iran
3 sabzevar
4 Pharmacology Research Center of Medicinal Plants, Mashhad University of Medical Sciences, Mashhad, Iran
5 Sabzevar University of Medical Sciences
6 Student Research Committee, Sabzevar University of Medical Sciences, Sabzevar, Iran
7 Cellular and Molecular Research Center, Department of Pharmacology, Sabzevar University of Medical Sciences, Sabzevar, Iran
8 Master of Nursing, Vice President of Research and Technology, Sabzevar University of Medical Sciences, Sabzevar, Iran
Abstract
Background: Cancer is considered as an important global health problem. Chemotherapy drugs used in cancer treatment have side effects such as bone marrow suppression, hair loss, gastrointestinal lesions, drug resistance, neurological dysfunction and heart toxicity. Flavonoids are bioactive compounds found in most plants and have beneficial effects on various cancerous tissues. Hesperidin is found as a flavonoid in the citrus family. In this research, the anticancer effects of hesperidin on different cancer cell lines, including adrenal medullary tumor (pheochromocytoma) (PC12), colon adenocarcinoma (SW48), prostate cancer (DU145) and esophageal squamous cell carcinoma (KYSE30) were investigated.
Methods: Cells were treated with different concentrations of hesperidin (0.75-100 μM) for 24, 48 or 72 hours, and cell viability was evaluated by MTT method. In addition, the expression of Bax, Bcl-2 and caspase-3 genes was measured using RT-PCR. Also, cell apoptosis was evaluated by flow-cytometry.
Results: The results showed that hesperidin prevents the proliferation of different cancer cells. Also, flow-cytometry results showed that hesperidin can increase cell apoptosis, which was confirmed by gene expression results. Hesperidin significantly decreased the expression of bcl-2 gene while increasing the expression of Bax and Caspase-3 (p<0.05).
Conclusion: It seems that hesperidin as a natural product can be used in the treatment of all types of human cancers in combination with chemical drugs.
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