Document Type : Original Article

Authors

1 PhD in Pharmacology, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

2 Professor, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

3 Assistant Professor, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

4 Associate Professor, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

5 PhD in Pathology, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran

6 Assistant Professor, Cancer Institute of Iran, Tehran University of Medical Sciences, Tehran, Iran

7 PhD in Biotechnology, Central Laboratory, Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran

Abstract

Introduction: In this study, we investigate the relationship between cancer and exposure to toxic substances in the environment. We used human encounters with them.
Materials and Methods: In this experimental study, 60 adult male mice were divided into four groups, including control, Malathion, Malathion with benzene and benzene. The control group was tested only with physiological serum. Except for the benzene group, all other groups were in skin contact with Malathion for 45 days was fed. After necropsy, tissue samples were taken from the target organs. In addition, cytotoxicity was assessed by MTT and micronucleus tests in vitro and the percentage of IC50 was determined. Microscopic observations of liver tissue showed hepatotoxicity and renal tissue renal toxicity in the Malathion group with benzene.
Results: The percentage of frequency of micronuclei at concentrations of 100 /g / ml, 50 /g / ml, 25 /g / ml was significant in all groups compared to the control group (P <0.05). In MTT test, the percentage of cell growth inhibition at concentrations of 100 g/ml, 50 g/ml, 25 g/ml, and 10 µg/ml in all groups was significantly different from the percentage of inhibition of cell growth with the control group (P < 0.05).
Conclusion: According to the findings of this study, certain doses of Malathion alone and in combination with benzene cause cytotoxicity and pre-carcinogenicity in cytotoxicity tests. Malathion exacerbates the carcinogenic effects of benzene

Keywords

Main Subjects

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