Document Type : Original Article
Authors
1 Department of Biology, Faculty of Sciences, Hakim Sabzevari University, Sabzevar, Iran
2 Department of Biology, Faculty of Basic Sciences, Gonbad kavous University, Gonbad kavous, Golestan, Iran
Abstract
Creating wounds from simple to advanced and from acute to chronic is one of the problems that mankind has faced for a long time. Over the past half century, with the discovery of antibiotics, human ability to manage wounds has improved much more than before, but antibiotic resistance has become a serious problem in recent decades, especially for chronic wounds, and therefore different therapeutic approaches are needed. Larval therapy is an old method that requires molecular cellular research and recognition and confirmation of its function can lead to the development of new drugs. In this study, the larvae of Lucilia sericata were first prepared and extracted, and then the extract was affected on fibroblast cells and molecular cellular studies were performed. The results indicate that TNF-α gene expression was 8 times higher in the control sample. The expression of SMAD-2 gene was 6 times higher in the control sample than in the control sample. TGF-β gene expression in the treated sample was 4 times that of the control sample. Given the additive effect on fibroblast growth and molecular confirmation of the above genes, it is suggested that this extract has the potential to heal wounds.
Keywords
Main Subjects
[8]. Wilson M, Nigam Y, Jung W, Knight J, Pritchard D. The impacts of larval density and protease inhibition on feeding in medicinal larvae of the greenbottle fly Lucilia sericata. Medical and veterinary entomology. 2016;30(1):17.