Document Type : Original Article
Authors
1 Assistant Professor, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran. 2
2 Associate Professor, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran.
3 M.Sc. Student, Department of Biotechnology, Institute of Science and High Technology and Environmental Sciences, Graduate University of Advanced Technology, Kerman, Iran
Abstract
Introduction: Stomach cancer and breast cancer are most common cancers in the world and in Iran. Today, the use of fungal and bacterial extractions has been interesting to cancer researchers. Rhizobium is a highly beneficial bacteria in agriculture and environment that provides the nitrogen needed for Legume family plants through coexistence with this family.
Materials and Methods: In this study, the effect of Bradyrhizobium cell extraction and its mutated type on the cancer of breast (MCF7) and stomach (AGS) was investigated. Material and Methods: Cytotoxicity effect of different concentration of Bradyrhizobium japonicum biomass and extract on stomach and breast cancer cells was investigated using MTT colorimetric method. Data analysis were conducted using Graphpad Prism 6.0 software and ANOVA method.
Results: The findings showed that both wild type and mutant type of bacteria had a significant meaningful effect on both types of cancer cells and their cytotoxicity was dependent on the concentration of extract or biomass (the lowest rate was observed at a concentration of 30 and at most 480 Micrograms per ml). In most cases biomass showed a better effect than extract.
Conclusion: In general, this study showed the effect of extract and biomass of Bradyrhizobium bacteria cytotoxicity on cancer cells, which could be used as a source for antineoplastic agent.
Keywords
Main Subjects
[2]. Anastas JN, Moon RT. WNT signalling pathways as therapeutic targets in cancer. Nat Rev Cancer. 2013; 13:11.
[17]. Mirzaei S, Batley, J, EI-Mellouki T, Liu S, Meksem K, Ferguson BJ, Gresshoff PM. Neodiversification of homeologous CLAVATA1-like receptor kinase genes in soybean leads to distinct developmental outcomes. Sci. Rep. 2017; 7:8878, doi: 10.1038/s41598-017-08252-y.