Document Type : Original Article
Authors
1 Msc, Department of Biology, Shiraz Branch, Islamic Azad University, Shira, Iran.
2 Associate Professor Department of Biology, Shiraz branch, Islamic Azad University, Shiraz, Iran
Abstract
Background & aim:Of different parts of Walnut plant in traditional diseases treatment such as diabetes are used. Given role of stresses in diabetes and chemical drugs’ side-effects in treating this disease, this study aimed to investigate alcoholic effects of walnut leaves extract on lipid profile in adult male rats’ diabetes with swimming stress was performed.
methods: This experimental study on 50 male rats matured divided into 5 groups: control, diabetic, diabetic treated with walnut leaves (200mg/kg), diabetes with swimming stress in water 15°C, diabetic treated with swimming stress and walnut leaves was done. The end, after 21 days injecting, weighing, and phlebotomizing rats, triglycerides serum levels, total cholesterol, LDL and HDL were measured and data using SPSS-18 software, ANOVA and Tukey were analyzed, so the significant data difference P
Keywords
Main Subjects
[1]. Karimzadeh K, Hosseini E, Kavoosi E, Nasihatkon A,Nikseresht M. Hypolipidemic effects of hydroalcoholic extract from walnut male flowers on diabetic rats.Armaghane danesh. 2013; 18 (6) :484-694.
[2]. Tripathi BK, Srivastava AK. Diabetes mellitus:complication and therapeutics. Med Sci Monit.2006; 12(2):130-47.
[3]. Chen J, Lu Y, Lee C, Li R, Leiter EH, Mathews CE,Commonalities of genetic resistance to
[4]. spontaneous autoimmune and free radical-mediated diabetes, Free Radic Biol Med 2008; 45(9): 1263-70
[5]. 22.Kumari S, Panda S, Mangaraj M, Mandal M, Mahapatra P .Plasma MDA and antioxidant vitamins in diabetic
retinopathy. Indian J Clin Biochem2008;23:158-62.
[6]. Asgary S, Rafieian-kopaei M, Goli-malekabadi N. The Effects of Jujube Fruit (Ziziphus vulgaris) Powder in Antioxidant Capacity Elevation and Prevent of Inflammation Detection Due to Diabetes in Wistar Rat.sjimu. 2016; 24 (5) :55-64.
[7]. Abolfathi A A, Vahabzadeh Z, Mahmoodiaghdam N,Vahabzadeh D, Hakhamanesh M S. Effects of taurine and
homocysteine on lipid profile and oxidative stress in fructose-fed rats. SJKU. 2017; 22 (3) :49-59
[8]. Bagheri P, Delshad Noghabi A, Moshki M. The Effect of Fasting on Metabolic Syndrome changes in type 2 Diabetic
People . Horizon Med Sci . 2014; 20 (1) :35-41.
[9]. Rezaei E, Hosseini ,, Mehrabani D. Effects of pomegranate juice on insulin and glucose in diabetic and non-diabetic
male rats. Journal of Birjand University of Medical Sciences. 2013; 20 (3) :244-251
[10]. Hosseini S, Karimzadeh K, Vessal M. Effects of a Hydroalcoholic Extract of Walnut Male Flowers on Diabetic Rats.zjrms 2013; 15 (11) :55-58
[11]. Papoutsi Z, Kassi E, Chinou I, Halabalaki M, Skaltsounis LA, Moutsatsou P. Walnut extract (Juglans regia L.) and its
component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity
in the cell line KS483. Br J Nutr. 2008 Apr;99(4):715-22.
[12]. Mirbadalzade R, Shirdel Z. Comparision of J. regia Shell Extract and Glibenclamide on Biochemical Blood Parameters in Alloxan-Induced Diabetic Rats.Journal of Experimental Animal Biology.2014;2(3):1-8.
[13]. Zhao MH, Jiang ZT, Liu T, Li R. Flavonoids in Juglans regia L. leaves and evaluation of in vitro antioxidant activity via intracellular and chemicalmethods. Scientific World J .2014; 2014: 303878.
[14]. Kumar S, Malhotra R, Kumar D. Antidiabetic and Free Radicals Scavenging Potential of Euphorbia hirta flower extract. Indian J Pharm Sci.2010; 72(4): 533–7.
[15]. Maurya AK, Tripathi S, Ahmed Z, Sahu RK.Antidiabetic and antihyperlipidemic effect of Euphorbia hirta in Streptozotocin induced diabetic rats. Scholars Res Library.2012; 4(2): 703-7.
[2]. Tripathi BK, Srivastava AK. Diabetes mellitus:complication and therapeutics. Med Sci Monit.2006; 12(2):130-47.
[3]. Chen J, Lu Y, Lee C, Li R, Leiter EH, Mathews CE,Commonalities of genetic resistance to
[4]. spontaneous autoimmune and free radical-mediated diabetes, Free Radic Biol Med 2008; 45(9): 1263-70
[5]. 22.Kumari S, Panda S, Mangaraj M, Mandal M, Mahapatra P .Plasma MDA and antioxidant vitamins in diabetic
retinopathy. Indian J Clin Biochem2008;23:158-62.
[6]. Asgary S, Rafieian-kopaei M, Goli-malekabadi N. The Effects of Jujube Fruit (Ziziphus vulgaris) Powder in Antioxidant Capacity Elevation and Prevent of Inflammation Detection Due to Diabetes in Wistar Rat.sjimu. 2016; 24 (5) :55-64.
[7]. Abolfathi A A, Vahabzadeh Z, Mahmoodiaghdam N,Vahabzadeh D, Hakhamanesh M S. Effects of taurine and
homocysteine on lipid profile and oxidative stress in fructose-fed rats. SJKU. 2017; 22 (3) :49-59
[8]. Bagheri P, Delshad Noghabi A, Moshki M. The Effect of Fasting on Metabolic Syndrome changes in type 2 Diabetic
People . Horizon Med Sci . 2014; 20 (1) :35-41.
[9]. Rezaei E, Hosseini ,, Mehrabani D. Effects of pomegranate juice on insulin and glucose in diabetic and non-diabetic
male rats. Journal of Birjand University of Medical Sciences. 2013; 20 (3) :244-251
[10]. Hosseini S, Karimzadeh K, Vessal M. Effects of a Hydroalcoholic Extract of Walnut Male Flowers on Diabetic Rats.zjrms 2013; 15 (11) :55-58
[11]. Papoutsi Z, Kassi E, Chinou I, Halabalaki M, Skaltsounis LA, Moutsatsou P. Walnut extract (Juglans regia L.) and its
component ellagic acid exhibit anti-inflammatory activity in human aorta endothelial cells and osteoblastic activity
in the cell line KS483. Br J Nutr. 2008 Apr;99(4):715-22.
[12]. Mirbadalzade R, Shirdel Z. Comparision of J. regia Shell Extract and Glibenclamide on Biochemical Blood Parameters in Alloxan-Induced Diabetic Rats.Journal of Experimental Animal Biology.2014;2(3):1-8.
[13]. Zhao MH, Jiang ZT, Liu T, Li R. Flavonoids in Juglans regia L. leaves and evaluation of in vitro antioxidant activity via intracellular and chemicalmethods. Scientific World J .2014; 2014: 303878.
[14]. Kumar S, Malhotra R, Kumar D. Antidiabetic and Free Radicals Scavenging Potential of Euphorbia hirta flower extract. Indian J Pharm Sci.2010; 72(4): 533–7.
[15]. Maurya AK, Tripathi S, Ahmed Z, Sahu RK.Antidiabetic and antihyperlipidemic effect of Euphorbia hirta in Streptozotocin induced diabetic rats. Scholars Res Library.2012; 4(2): 703-7.
[16]. Habibian M, Saghafi M R,Farzanegi P. The Effect of Regular Swimming Exercise on the Levels of Renal Matrix
Mettaloproteinase-2 and Transforming Growth Factor -β1 in Rats with Diabetes. Journal of Kerman University of
Medical Sciences. 2016;23(4): 446-456.
[17]. Teimoori M, Montaser Kouhsari Sh,Ghafarzadegan R,Hajiaghaee R. Anti-diabetic effects of juglans regia leaves
methanolic extract on alloxan-induced male wisrar rats. JMed Plants.2010; 2(34): 142-9.
[18]. Solar A, Colaric M, Usenik V, Stampar F.Seasonal variations of selected flavonoids, phenolic acids and quinones in annual shoots of common walnut (Juglans regia L.). Plant Sci. 2006; 170(3):453-61.
[19]. Mohammadi J, Delaviz H, Malekzadeh JM, Roozbehi A.The effect of hydro alcoholic extract of Juglans regia leaves
in streptozotocin-nicotinamide induced diabetic rats. PakJ Pharm Sci. 2012 Apr;25(2):407-11.
[20]. Habibian M,Farzanegi P,Azimy GR.Therapeutic effect of swimming training and arbutin supplement on diabetesinduced renal oxidative stress. Journal of Daneshvar Medicine . 2015; 22 (114) :13-20
[21]. Salehi I, Mohammadi M, Farajnia S, Gaderi Sophi F,Badalzadeh R, Vatankhah A M. Effect of Regular
Swimming on Oxidative Stress and Atherogenic Index in Blood of Diabetic Male Rats. Sci J Hamadan Univ Med Sci . 2007; 14 (3) :29-3.
[22]. Wallace K, Burt AD, Wright MC. Liver fibrosis. Biochem J.2008 Apr 1;411(1):1-18
[23]. Shahraki M, Mirshekari H, shahraki A, Khamar moghadam S, shahraki E. The Survey of Mandatory Cold Swim Stress Insulin in Male Rats on FBS, OGTT and Serum Insulin in Male Rats. Sjimu. 2013; 21 (1) :8-15.
[24]. Petznick A. Insulin management of type 2 diabetes mellitus. American Family Physician July 2011; 84: 183-90.
[25]. Neamat Pour S, Shahbazian HB, Gholam Pour A.Evaluation of Psychological Problems in Diabetic Patients.2010; Journal of the Jundishapur Scientific Medical, 4(9):354-360.
[26]. Young E and Unachukwu C. 2012. Psychosocial aspects of diabetes mellitus. Journal of Diabetes Medicine 2012; 20(1):5-7.
[27]. Lee H, Ahn S, Kim Y. Self – efficacy ,and glycemic control of Korans with diabetesus. Journal of the Asian Nurse Res
2009; 3(3): 139- 46.
[28]. Tapsell LC, Gillen LJ, Patch CS, Batterham M, Owen A,Bare ́ M, et al.Including walnuts in a low-fat modifiedfatdiet
improves HDL cholesterol-to-total cholesterol ratios in patients with type 2 diabetes.Diabetes Care. 2004;27(12):2777-83.
[29]. Sabate J, Ang Y. Nuts and health outcomes: newepidemiologic evidence.AmJ Clin Nutr 2009; 89(5): 1643S-1648S.
[30]. Shahraki M, Mirshekari H, shahraki A, Khamarmoghadam S, shahraki E. The Survey of Mandatory Cold
Swim Stress Insulin in Male Rats on FBS, OGTT and Serum Insulin in Male Rats. sjimu. 2013; 21 (1) :8-15
[31]. Hosseini S, Jamshidi L, Mehrzadi S, Mohammad K,Najmizadeh AR, Alimoradi H, Huseini HF. Effects of Juglans regia L. leaf extract on hyperglycemia and lipid profiles in type two diabetic patients: a randomized double-blind, placebo-controlled clinical trial. JEthnopharmacol. 2014 Mar 28;152(3):451-6.
[32]. Reza-Mohtasham S, Nazem H, Fazilati M. Effect of alcoholic extract of Juglans regia leave on brain antioxidant enzymes in streptozotocin-induced diabetic rat. KAUMS Journal ( FEYZ ). 2016; 20 (3) :214-221.
[33]. Rathod NR, Chitme HR, Irchhaiya R, Chandra R.Hypoglycemic Effect of Calotropis gigantean Linn. Leaves
and Flowers in Streptozotocin-Induced Diabetic Rats.Oman Med J. 2011 Mar;26(2):104-8.
[34]. Wang Y, Campbell T, Perry B, Beaurepaire C, Qin L.Hypoglycemic and insulin-sensitizing effects of berberine
in high-fat diet- and streptozotocin-induced diabetic rats. Metabolism. 2011 Feb;60(2):298-305.
[35]. Karra E, Yousseif A, Batterham RL.Mechanisms facilitating weight loss and resolution of type 2 diabetes
following bariatric surgery.Trends Endocrinol Metab. 2010 Jun;21(6):337-44.
[36]. Mahmoudzadeh-Sagheb H, Heidari Z, Shahraki MR.Effects of cold water swim stress on volumetric parameters
of adrenal gland in rats. Zahedan J Res Med Sci (ZJRMS).2011;13(2): 44-48.
[37]. Divband K, Komeili G, Saeidi-Neek F. Effects of Walnut leaves aqueous extract on blood sugar and serum lipids in
diabetic rats. J Birjand Univ Med Sci. 2010; 17 (1) :11-18.
[38]. Solar A , Mateja Colarič M, Valentina Usenik V, Stampar F. Seasonal variations of selected flavonoids, phenolic
acids and quinones in annual shoots of common walnut (Juglans regia L.). Plant Science.2006;170(3): 453–461.
Mettaloproteinase-2 and Transforming Growth Factor -β1 in Rats with Diabetes. Journal of Kerman University of
Medical Sciences. 2016;23(4): 446-456.
[17]. Teimoori M, Montaser Kouhsari Sh,Ghafarzadegan R,Hajiaghaee R. Anti-diabetic effects of juglans regia leaves
methanolic extract on alloxan-induced male wisrar rats. JMed Plants.2010; 2(34): 142-9.
[18]. Solar A, Colaric M, Usenik V, Stampar F.Seasonal variations of selected flavonoids, phenolic acids and quinones in annual shoots of common walnut (Juglans regia L.). Plant Sci. 2006; 170(3):453-61.
[19]. Mohammadi J, Delaviz H, Malekzadeh JM, Roozbehi A.The effect of hydro alcoholic extract of Juglans regia leaves
in streptozotocin-nicotinamide induced diabetic rats. PakJ Pharm Sci. 2012 Apr;25(2):407-11.
[20]. Habibian M,Farzanegi P,Azimy GR.Therapeutic effect of swimming training and arbutin supplement on diabetesinduced renal oxidative stress. Journal of Daneshvar Medicine . 2015; 22 (114) :13-20
[21]. Salehi I, Mohammadi M, Farajnia S, Gaderi Sophi F,Badalzadeh R, Vatankhah A M. Effect of Regular
Swimming on Oxidative Stress and Atherogenic Index in Blood of Diabetic Male Rats. Sci J Hamadan Univ Med Sci . 2007; 14 (3) :29-3.
[22]. Wallace K, Burt AD, Wright MC. Liver fibrosis. Biochem J.2008 Apr 1;411(1):1-18
[23]. Shahraki M, Mirshekari H, shahraki A, Khamar moghadam S, shahraki E. The Survey of Mandatory Cold Swim Stress Insulin in Male Rats on FBS, OGTT and Serum Insulin in Male Rats. Sjimu. 2013; 21 (1) :8-15.
[24]. Petznick A. Insulin management of type 2 diabetes mellitus. American Family Physician July 2011; 84: 183-90.
[25]. Neamat Pour S, Shahbazian HB, Gholam Pour A.Evaluation of Psychological Problems in Diabetic Patients.2010; Journal of the Jundishapur Scientific Medical, 4(9):354-360.
[26]. Young E and Unachukwu C. 2012. Psychosocial aspects of diabetes mellitus. Journal of Diabetes Medicine 2012; 20(1):5-7.
[27]. Lee H, Ahn S, Kim Y. Self – efficacy ,and glycemic control of Korans with diabetesus. Journal of the Asian Nurse Res
2009; 3(3): 139- 46.
[28]. Tapsell LC, Gillen LJ, Patch CS, Batterham M, Owen A,Bare ́ M, et al.Including walnuts in a low-fat modifiedfatdiet
improves HDL cholesterol-to-total cholesterol ratios in patients with type 2 diabetes.Diabetes Care. 2004;27(12):2777-83.
[29]. Sabate J, Ang Y. Nuts and health outcomes: newepidemiologic evidence.AmJ Clin Nutr 2009; 89(5): 1643S-1648S.
[30]. Shahraki M, Mirshekari H, shahraki A, Khamarmoghadam S, shahraki E. The Survey of Mandatory Cold
Swim Stress Insulin in Male Rats on FBS, OGTT and Serum Insulin in Male Rats. sjimu. 2013; 21 (1) :8-15
[31]. Hosseini S, Jamshidi L, Mehrzadi S, Mohammad K,Najmizadeh AR, Alimoradi H, Huseini HF. Effects of Juglans regia L. leaf extract on hyperglycemia and lipid profiles in type two diabetic patients: a randomized double-blind, placebo-controlled clinical trial. JEthnopharmacol. 2014 Mar 28;152(3):451-6.
[32]. Reza-Mohtasham S, Nazem H, Fazilati M. Effect of alcoholic extract of Juglans regia leave on brain antioxidant enzymes in streptozotocin-induced diabetic rat. KAUMS Journal ( FEYZ ). 2016; 20 (3) :214-221.
[33]. Rathod NR, Chitme HR, Irchhaiya R, Chandra R.Hypoglycemic Effect of Calotropis gigantean Linn. Leaves
and Flowers in Streptozotocin-Induced Diabetic Rats.Oman Med J. 2011 Mar;26(2):104-8.
[34]. Wang Y, Campbell T, Perry B, Beaurepaire C, Qin L.Hypoglycemic and insulin-sensitizing effects of berberine
in high-fat diet- and streptozotocin-induced diabetic rats. Metabolism. 2011 Feb;60(2):298-305.
[35]. Karra E, Yousseif A, Batterham RL.Mechanisms facilitating weight loss and resolution of type 2 diabetes
following bariatric surgery.Trends Endocrinol Metab. 2010 Jun;21(6):337-44.
[36]. Mahmoudzadeh-Sagheb H, Heidari Z, Shahraki MR.Effects of cold water swim stress on volumetric parameters
of adrenal gland in rats. Zahedan J Res Med Sci (ZJRMS).2011;13(2): 44-48.
[37]. Divband K, Komeili G, Saeidi-Neek F. Effects of Walnut leaves aqueous extract on blood sugar and serum lipids in
diabetic rats. J Birjand Univ Med Sci. 2010; 17 (1) :11-18.
[38]. Solar A , Mateja Colarič M, Valentina Usenik V, Stampar F. Seasonal variations of selected flavonoids, phenolic
acids and quinones in annual shoots of common walnut (Juglans regia L.). Plant Science.2006;170(3): 453–461.
)