The survey of contamination with genital mycoplasma among infertile women with bacterial vaginosis by PCR method

Ahmadiyan Fard, Zohreh; Moazami, Amin

Document Type : Original Article

Authors

¹ Midwife, MSc in Microbiology, Islamic Azad University of Sirjan, Sirjan, Iran

² 2. MSc in Microbiology, Dept. of Nursing, Islamic Azad University of Sirjan, Sirjan, Iran

Abstract

Background and Objectives: Ureaplasma urealyticum and Mycoplasma genitalium are the most important opportunistic pathogens in the female reproductive system and the causative agent of the pelvic infection, non-gonococcal urethritis, abortion and infertility. The aim of this study was to frequency of Mycoplasma genitalium and Ureaplasma urealyticum among infertile women with vaginal infection by PCR in Kerman.
Materials and Methods: In this cross-sectional study, the endo cervical swab sample from 60 women with vaginal infection (vaginitis and cervicitis) referred to the diagnostic laboratories of infertility treatment centers in Kerman in 2013 was prepared. The specimens were transferred to the laboratory in the transport medium and the DNA extracted from the specimens was analyzed as a template for amplification of the 16S rRNA encoding gene in the PCR reaction. Data were analyzed by SPSS V.21 software.
Results: Among 60 samples, 38% (23sample) of the samples had mycoplasma contamination and Ureaplasma urealyticum and Mycoplasma genitalium bacteria were found in 13.32% (8 sample) and 11.6% (7 sample) of patients, respectively.
Conclusion: Considering the potential effects of mycoplasmas on the complications of infection in maternal pregnancies and infant mortality, Therefore, the need for rapid diagnosis of this infection is felt more than ever. From the two microbial agents studied in this study, the possibility of infertility caused by infection with Ureaplasma urealyticum increased significantly, but more studies are needed

Keywords

Main Subjects

Microbiology

References

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Journal of Sabzevar University of Medical Sciences

The survey of contamination with genital mycoplasma among infertile women with bacterial vaginosis by PCR method

References

References

Volume 26, Issue 6
March and April 2020
Pages 789-795

The survey of contamination with genital mycoplasma among infertile women with bacterial vaginosis by PCR method

References

References

Volume 26, Issue 6March and April 2020Pages 789-795

Volume 26, Issue 6
March and April 2020
Pages 789-795