Detection of expression of novel long non-coding RNA lncUSMycN in breast tissues and its overexpression in women with breast invasive ductal carcinoma in East Azerbaijan province

Ravanbakhsh Gavgani, Reyhaneh; Babaei, Esmaeil; Hosseinpourfeizi, Mohammad Ali; Montazeri, Vahid

Document Type : Original Article

Authors

¹ Department of Biological Sciences, School of Natural Sciences, University of Tabriz, Tabriz, Iran

² Department of Thoracic Surgery, Nour-nejat Hospital, Tabriz, Iran

Abstract

Introduction: Breast cancer is the most common tumor malignancy among women worldwide. Studies have revealed new class of RNA molecules named Long non-coding RNAs that play important role in tumor development, progression and metastasis. Therefore, this study aimed to evaluate long non-coding RNA lncUSMycN expression in breast cancer.
Methods: In this study, 40 breast tumor with invasive ductal carcinoma and 40 normal marginal tissues were collected and after RNA extraction using TRizol kit, cDNA was synthesized. Expression level of lncUSMycN was obtained by applying qRT-PCR method. In order to evaluate association of lncUSMycN expression in tumor compared to normal tissues REST software was used. Biomarker potential of lncUSMycN was evaluated by drawing ROC curve using SigmaPlot. In addition, relationship between lncUSMycN expression and clinicopathological features was analysed.
Results: Results from REST indicated significant upregulation of lncUSMycN in tumor tissues compared to normal marginal specimens (95% CI, p =0.001). ROC curve analysis demonstrated the biomarker potential of lncUSMycN (ROCAUC =0.72, p=0.0006). Evaluation of the relationship between lncUSMycN expression and clinicopathological features revealed that there are significant association between lncUSMycN expression and early stages (95% CI, P=0.005) and well-differentiated tumors (95% CI, P=0.046).
Conclusion: Considering upregulation of lncUSMycN expression in invasive ductal carcinoma this lncRNA may be probably considered as a new potential diagnostic biomarker for breast cancer.

Keywords

Main Subjects

genetics

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Journal of Sabzevar University of Medical Sciences

Detection of expression of novel long non-coding RNA lncUSMycN in breast tissues and its overexpression in women with breast invasive ductal carcinoma in East Azerbaijan province

References

References

Volume 26, Issue 3
September and October 2019
Pages 285-292

Detection of expression of novel long non-coding RNA lncUSMycN in breast tissues and its overexpression in women with breast invasive ductal carcinoma in East Azerbaijan province

References

References

Volume 26, Issue 3September and October 2019Pages 285-292

Volume 26, Issue 3
September and October 2019
Pages 285-292