The effect of exercise training type on 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine levels in the brain and liver of rats

TaheriChadorneshin, Hossein; Afzalpour, Mohammad Esmaiel; Afroozi-Gerow, Ehsan; Abtahi-Eivary, Seyed Hosein; Alipour-Raz, Meysam

Document Type : Original Article

Authors

¹ Department of Sport Sciences, University of Bojnord, Bojnord, Iran

² Department of Sport Sciences, University of Birjand, Birjand, Iran

³ Department of Sport Sciences, University of Birjand, Birjand, Iran.

⁴ Department of Clinical Biochemistry, Gonabad University of Medical Sciences, Gonabad, Iran.

Abstract

Reactive oxygen species (ROS) result in serious damage oxidation of proteins, lipids and genomic structures. Studies have shown that production of ROS increases during intensive exercise training. The aim of present study was to investigate the effect of two type sprint and endurance exercise trainings on 8-oxoguanine DNA glycosylase (OGG1) and hydroxy-2'-deoxyguanosine (8-OHdG) levels in the brain and liver of Wistar rats. 24 adult Albino Wistar rats were randomly divided into sedentary control, sprint exercise training, and endurance exercise training groups. Rats in sprint and endurance exercise training groups ran on treadmill for 6 weeks, 6 days per week, at 80 to 100 percent of maximal oxygen consumption. OGG1 and 8-OHdG levels were measured using sandwich ELISA assay. Data analyzed using one way ANOVA at P≤0.05 level. Results showed that both of sprint and endurance exercise trainings result in significant increase in OGG1 levels in brain and liver. Sprint exercise training resulted in greater increase in brain OGG1 than endurance exercise training. However, sprint and endurance exercise training had no significant effect on 8-OHdG levels in brain and liver tissues. OGG1 content correlated negatively with 8-OHdG levels. Collectively, sprint and endurance exercise trainings prevents from genomic structure through an increase in OGG1 contents.

Keywords

Main Subjects

Physical Education

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Journal of Sabzevar University of Medical Sciences

The effect of exercise training type on 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine levels in the brain and liver of rats

References

References

Volume 26, Issue 1
March and April 2019
Pages 99-107

The effect of exercise training type on 8-oxoguanine DNA glycosylase and 8-hydroxy-2'-deoxyguanosine levels in the brain and liver of rats

References

References

Volume 26, Issue 1March and April 2019Pages 99-107

Volume 26, Issue 1
March and April 2019
Pages 99-107