Document Type : Original Article
Authors
1 Department of Biology, The Biology teacher of Qaemshahr Education Office, Qaemshahr, IRAN
2 Assistant Professor in Animal Physiology, Department of Biology, Islamic Azad University, Qaemshahr Branch, qaemshahr, Iran
Abstract
In the last few decades, research has been focused on the use of natural products for cancer therapy, such as crude plant extracts or some of different plant secondary metabolits. The objective of this study was to examine the invitro cytotoxic activities of crude ethanolic extract of leaf and flower buds of Crataegus melanocarpa, on human MCF-7 and HeLa cell lines, with different concentrations, by Dye exclusion and Micro culture tetrazolium test (MTT) assay. The optical density (OD) colored solution was quantified at 570 nm wavelengths by an ELISA Reader, after 24, 48 and 72 h incubation. The results showed that ethanolic extracts, after 48h incubation, at in 5 and 10 mg/ml suppressed the proliferation of cancerous MCF-7 cells by significant diffrence with control group (p
Keywords
Main Subjects
- Parsa n. The cellular and molecular basis of cancer in humans. Journal of Cell and Texture. 2011; Vol. 2, No. 4, 376-365. [In Persian]
- Yavari P, Mehrabi Y, Pourhoseinqoli MA. Awareness and action of women toward breast self-examination: a case-control study. J Ardabil Univ Med Sci. 2006;5(4):371-7. [In Persian].
- Aghamolaei T, Hasani L, Tavafian SS, Zare S. Improving Breast self-examination: an educational intervention based on health belief model. Iran J Cancer Prev. 2011;4(2):82-7.
- Harirchi I, Ebrahimi M, Zamani N, Jarvandi S, Montazeri A. Breast cancer in Iran: a review of 903 case records. Public Health. 2000; 114(2):143–145.
- Akbari ME, Mozaffar M, Heidari A, Zirakzadeh H, Akbari A, Akbari M, et al. Recurrence and Survival Effect in Breast Conserving Surgery: What are the Predictive and/or Prognostic Factors? Iran J Cancer Prev. 2011;4(2):49-54.
- Hacker N. Cervical cancer. In: Berek J, Hacker N. Practical gynecologic oncology. 4th ed. Philadelphia: Lippincott Williams & Wilkins; 2005; 337-386.
- Chi D, Abu-Rustum N, Hoskins W. Cancer of cervix.In: Rock J, Jones H. Telinde's operative gynecology. 9th ed. Philadelphia: Lippincott Williams & Wilkins; 2003; 13731444.
- Aynehchi, Y. Pharmacognosy and medicinal plants of Iran. Tehran: University Pub. 1986; p: 387-91.[In Persian]
- Rodrigues S, Calhelha RC, Barreira JCM, Duenas M, Carvalho AM, Abreu RMV, Santos-Buelga C, Ferreira ICFR Crataegus monogyna buds and fruits phenolic extracts: growth inhibitory activity on human tumour cell lines and chemical characterization by HPLC–DAD–ESI/MS. Food Res Int. 2012; 49: 516–523. doi: 10.1016/j.foodres.2012.07.046
- Lingrong W, Xingbo G, Rui HL, Lijun Y, Arshad MA, Xiong Fu. Phenolic contents and cellular antioxidant activity of Chinese hawthorn “Crataegus pinnatifida”. Food chem. 2015; 186: 54-62.
- Kao ES, Wang CJ, Lin WL, Chu CY, and Tseng TH. Effects of polyphenols derived from fruit of Crataegus pinnatifida on cell transformation, dermal edema and skin tumor formation by phorbol ester application. Food Chem Toxicol. 2007; 45: 1795-1804.
- Zhang Y, Li HW, Sun JP. Extraction-Separation of total flavone from the fruit of Crataegus pinnatifida and anticancer activities in vitro. Chin. Tradit. Herbal Drugs. 2004; 35: 787–789.
- Min BS, Kim YH, Lee SM, Jung HJ, Lee JS, Na MK, Lee CO, Lee JP, Bae K. Cytotoxic triterpenes from Crataegus pinnatifida. Arch Pharm Res. 2000; 23: 155-158.
- Numata A, Yang P, Takahashi C, Fujiki R, Nabae M, Fujita E. Cytotoxic triterpenes from a Chinese medicine, goreishi. Chem Pharm Bull. 1989; 37: 648–651.
- Matsukawa Y, Marui N, Sakai T, Satomi Y, Yoshida M, Matsumoto K, Nishino H, Aoike A. Genistein arrests cell cycle progression at G2-M. Cancer Res. 1993; 53:1328–1331. [PubMed: 8443813]
- Ramful D, Aumjaud B, Neergheen VS, Soobrattee MA, Googoolye K, Aruoma OI, Bahorun T. Polyphenolic content and antioxidant activity of Eugenia pollicina leaf extract in vitro and in model emulsion systems. Food Res. Int. 2011; 44: 1190-1196.
- Gomes CA, Cruz TG, Andrade JL, Milhazes N, Borges F, Marques MPM. Anticancer Activity of Phenolic Acids of Natural or Synthetic Origin: A Structure-Activity Study. J Med Chem. 2003;46: 5395-5401
- Ebrahimzadeh, M.A. and Bahramian, F. Antioxidant activity of Crataegus pentaegyna subsp. elburensis fruits extracts used in traditional medicine in Iran. Pakistan J Biol Sci. 2009; 12(5): 413–419.
- Bahri-Sahloul R, Ammar S, Fredj RB, Saguem S, Grec S, Trotin F, Skhiri FH. Polyphenol contents and antioxidant activities of extracts from flowers of two Crataegus azarolus L. varieties. Pakistan J Biol Sci. 2009; 12(9): 660-668.
)