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Assessment of antibacterial effect of nano polyamidoamine-G5 (NPAMAM-G5) dendrimer on escherichia coli, proteus mirabilis, salmonella typhi, bacillus subtilis and staphylococcus aureus isolated from the hospital environment

Document Type : Original Article

Authors

1 M.Sc., School of Public Health, Qom University of Medical Sciences, Qom, Iran

2 Expertis, Khalkhal Faculty of Medical Sciences; Member of Student Research Committee, Ardabil University of Medical Sciences, Ardabil, Iran

3 M.Sc in Bacteriology, School of Medical, Ardabil University of Medical Sciences, Ardabil, Iran

4 B.Sc. in Nursing, Ministry of Health and Medical Education, Tehran, Iran

5 B.Sc. in Nursing, Islamic Azad University, Ardabil University of Medical Sciences, Ardebil, Iran

6 B.Sc. in Environmental Health Engineering, School of Public Health and Student Research Committee, Ardabil University of Medical Sciences, Ardabil, Iran

Abstract

Background & Objectives: This study aimed to examine the antibacterial effect of Nano Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus isolated from the hospital environment.
Materials & Methods:In this study, the antibacterial effects of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Different concentrations of Polyamidoamine-G5 inoculated onto blank disks and were placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of NPAMAM-G5 dendrimer were determined by micro-dilution method.
Results: Zone of inhibition in Polyamidoamine-G5 dendrimers concentration of 25 μg/mL for Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus were 22, 28, 21, 16 and 20 mm, respectively. Regarding the zone of inhibition in gram negative bacteria with gram positive ones was p=0.16 and was not significant different. The minimum inhibitory concentration for both Proteus Mirabilis and Salmonella Typhi was 2.5 μg/mL and for Escherichia Coli, Bacillus Subtilis and Staphylococcus Aureus was 25 μg/mL. The Minimum Bactericidal Concentration (MBC) for Salmonella Typhi was 50 μg/mL and for all Proteus Mirabilis, Escherichia Coli, Bacillus Subtilis and Staphylococcus Aureus was 100 μg/mL.     
 Conclusion: According to the results, NPAMAM-G5 dendrimer can effectively eliminate Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus.

Keywords

References
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Journal of Sabzevar University of Medical Sciences
Volume 24, Issue 6
March and April 2018
Pages 112-120
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  • Receive Date: 18 March 2018
  • Accept Date: 18 March 2018
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