Microbiology
mohammad bokaeian; hamed tahmasebi; Javad adabi; alireza mohammad zadeh; jalal mardaneh
Volume 25, Issue 1 , May and June 2018, , Pages 31-37
Abstract
Background: Bacterial resistants to beta-lactam antibiotics, are steadily expanding. Excessive consumption beta-lactam antibiotics in the treatment of related Staphylococcus aureus infections caused the emergence of beta-lactamase enzymes in strains. Identification and analysis of molecular characteristics ...
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Background: Bacterial resistants to beta-lactam antibiotics, are steadily expanding. Excessive consumption beta-lactam antibiotics in the treatment of related Staphylococcus aureus infections caused the emergence of beta-lactamase enzymes in strains. Identification and analysis of molecular characteristics of these strains can be effective to select on the appropriate antibiotic treatment.Materials and Methods: Initially, the isolates collected from clinical samples were screened by biochemical tests. After the whole Staphylococcus aureus resistance to beta-lactam antibiotics, were evaluated by used of molecular-specific primers.Results: Of 496 isolates obtained from different clinical samples, 147 isolates were identified as S. aureus. The highest resistance to the antibiotics had related to penicillin, Oxacillin, Cefocithin and the lowest resistance antibiotics had related to Vancomycin. From the 147 samples, 143 samples were blaZ genes that allocated to over 97 percent.Conclusion: According to the results of molecular tests, in the Zahedan spread of carrier-lactamase strains is very high. This would be to treat all staphylococcal infections of beta lactam antibiotics are not used as much as possible to the access to early treatment prevented the increase in resistant strains.
Microbiology
maryam alsadat zarei; Mahboubeh Madani
Volume 25, Issue 1 , May and June 2018, , Pages 151-160
Abstract
Background: Teucrium polium is a medicinal plant which species have been used for over 2000 years in traditional medicine. The aim of this study was evaluation of the effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in mice tissue. Material and Method: The study was performed ...
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Background: Teucrium polium is a medicinal plant which species have been used for over 2000 years in traditional medicine. The aim of this study was evaluation of the effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in mice tissue. Material and Method: The study was performed on 90 mature male mices (9 per group), that were devided into 10 groups: normal, placebo, 3 treatment group (candida- extract), control positive (Candida glabrata) and control negative (extract). Treatment groups were received 50,100 and 200 mg/kg dosages of extract for 20 days (every other day) by intraperitoneal injection. At the end of injections, homogenized liver, spleen and kidney were cultured on SDA medium and then colony count were performed. Results: The results showed, that liver in 100 mg/kg, spleen and kidney in 200mg/kg haven’t any yeast. Discussion and Conclusion: According to the results, Teucrium polium extract, perhaps can stimulat the immunity against Candida glabrata. Keywords: Teucrium polium, Candida glabrata, liver, spleen, kidney
Microbiology
vajiheh gharzi; sahar kalate; rahim akrami; Mohammad Shurideh yazdi; Maryam Latifnia; Mojtaba Fattahi Abdizadeh
Volume 24, Issue 6 , March and April 2018, , Pages 131-137
Abstract
Evaluation of immune response to HBV vaccine in the staff and students of Sabzevar University of Medical Sciences (2014-15) Background:Hepatitis B is a bloodborne infections and it is predicted that about two billion people in the world have been exposed to the hepatitis B virus. Because of occupational ...
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Evaluation of immune response to HBV vaccine in the staff and students of Sabzevar University of Medical Sciences (2014-15) Background:Hepatitis B is a bloodborne infections and it is predicted that about two billion people in the world have been exposed to the hepatitis B virus. Because of occupational reasons, medical students and staffs are more susceptible to expose to this disease than other members of society. Materials and Methods:This study is a descriptive-analytic study and 238 staffs and students of Sabzevar University of Medical Sciences were randomly selected and sampled. Then, the antibody titer of surface anti-protein of hepatitis B virus (HBs- Ab) was measured using ELISA method and the results were analyzed using the software Stata 12 after recording the demographic results and information. Results:Out of 238, 156 persons were female ( 65.5%) and 82 persons ( 34.5%) were male. 66% of individuals had antibody higher than 10 mu/ml and 37.4 % of individuals showed the appropriate answers higher than 100 u/ml. The relationship between job, sex and marital status with the immune response was significant. 100% of people who received full dose along with the booster vaccine were immune. Over time, the percentage of immune people has been decreased. Conclusion:Considering the reduction of response to vaccines over time and healthcare personnel being at risk, it is recommended that antibody titer was periodically examined in these individuals in 5 to 10 years to administer a booster dose in the case of a decrease in antibody titers.