genetics
Akram Rahimi-Moghaddam; Nassim Ghorbanmehr; Sedigheh Gharbi; Fatemeh Nili
Abstract
Background: The most common cause of cervical cancer is human papillomavirus, which induces its carcinogenic properties on cells through two oncoproteins named E6 and E7. Viral miRNA and oncogenes can alter the expression levels of human miRNAs and genes. Examining the expression profile of miRNAs and ...
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Background: The most common cause of cervical cancer is human papillomavirus, which induces its carcinogenic properties on cells through two oncoproteins named E6 and E7. Viral miRNA and oncogenes can alter the expression levels of human miRNAs and genes. Examining the expression profile of miRNAs and their target genes in cervical cancer leads to the identification of miRNAs and genes that can be used as diagnostic biomarkers or therapeutic targets. MAP9 is one of the predicted targets of HPV16-miR-H2-1. In this study, changes in the expression level of MAP9 and a human miRNA regulating MAP9 are investigated in cervical cancer, and their potential as diagnostic biomarkers or therapeutic targets is evaluated.
Materials and methods: After predicting miRNAs regulating MAP9 using miRDB server, one of these miRNA assosiated with squamous cell carcinoma was selected for quantification in clinical samples. Formalin-fixed, paraffin-embedded (FFPE) blocks of cervical tissues from 30 patients with squamous cell carcinoma were used. Deparaffinization, RNA extraction, DNase treatment, and cDNA synthesis were performed for each sample. The expression level of selected miRNA and MAP9 in tumor and normal samples was investigated by Real-Time PCR method. The results were statistically analyzed.
Results: The significant upregulation of hsa-miR-142-5p and downregulation of MAP9 were observed in tumor samples compared with normal tissues. Roc curve analysis showed that hsa-miR-142-5p and MAP9 have high diagnostic capability for cervical cancer (AUC are 0.80 and 0.81 respectively).
Conclusion: hsa-miR-142-5p and MAP9 have the potential to be used as diagnostic biomarkers or therapeutic targets for cervical cancer.
genetics
SAEED PIRMORADI
Abstract
Introduction: Autism is a set of environmental and genetic disorders in the nervous system that result in defects in social behaviors, social communication, stereotyped behaviors and difficulty in motor skills and inability to plan motor. These factors are the severity of the disease and how affect its ...
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Introduction: Autism is a set of environmental and genetic disorders in the nervous system that result in defects in social behaviors, social communication, stereotyped behaviors and difficulty in motor skills and inability to plan motor. These factors are the severity of the disease and how affect its response to treatment.Materials and Methods: In this study, we sought to identify a common list of different genes expressed (DEG) using a meta-analysis method by bioinformatics tools. Three microwave studies were identified, including 109 samples, of which 90 were sick and 19 were healthy. These studies were analyzed by software and meta-analysis was performed on them.Results: After isolation of genes with different expression with the help of statistical analysis by R software, genes (EIF1AY, EIF2S3, IL32, ARPC4-TTLL3, LILRA5, EIF5A, XIST, RARA, TXLNG,) were obtained and then by examining their gene ontology from the final results were obtained through the enrichr database and the association of their interaction pathways with pathways and interaction networks with other genes involved in autism.Conclusion: By identifying genes with different expression in different studies that had a significant decrease or increase in expression and examining them in biological, molecular and cellular pathways in general, it was found that this set of genes can be used in autism and some pathways in the functional process. This disease has a role, so it is possible to provide desirable treatment strategies to control them by examining the targets of their effects and products.