Abolfazel Mossavi; Mohammad Armin; Hashem Akhlaghi
Volume 28, Issue 1 , January and February 2021, , Pages 83-88
Abstract
Introduction: Typical nanoparticle synthesis methods have disadvantages such as poor structure, low production rate, high production cost and high energy requirement. Therefore, today the tendency to use plant resources as one of the most suitable methods of nanoparticle synthesis is taken into consideration ...
Read More
Introduction: Typical nanoparticle synthesis methods have disadvantages such as poor structure, low production rate, high production cost and high energy requirement. Therefore, today the tendency to use plant resources as one of the most suitable methods of nanoparticle synthesis is taken into consideration by researcher attention. Foeniculum vulgare is one of the most important medicinal plants in Iran that has the potential to synthesize nanoparticles from its seed or shoot extracts. In this study, the possibility of green synthesis of zero iron nanoparticles using fennel seed extracts was investigated. Materials and Methods: In this study, seed extract was used to synthesize green nanoparticles of iron particles.The antioxidant properties and the total amount of phenolic compounds of seed extract and extract containing nano-iron particles were determined. Structural analysis of iron nanoparticles was performed using scanning electron microscopy. Results: The formation of iron nanoparticles was confirmed by changing the color of the solution from yellow to brown. Both in the extract containing the seeds and in the extract containing iron nanoparticles, antioxidant properties were observed and adding iron nanoparticles increased the antioxidant properties of fennel. Structural analysis of iron nanoparticles using scanning electron microscopy showed that the shape of spherical particles and its dimensions are 200 nm. Conclusion: The results of this experiment showed that the fennel seed extract can be used to synthesize nanoscale zero-valent iron particles, and increasing the Zero nanoparticles increases its antioxidant properties. Received
Biotechnology & nanotechnology
fereshteh jookar kashi; Zohreh Boroumand
Volume 28, Issue 1 , January and February 2021, , Pages 144-155
Abstract
Introduction: The bacteria with a remarkable ability to regenerate heavy metal ions are considered biological factories or important nano-factories to produce nanoparticles. The bacterial production of nanoparticles as a green and one-step method can overcome production problems and disadvantages through ...
Read More
Introduction: The bacteria with a remarkable ability to regenerate heavy metal ions are considered biological factories or important nano-factories to produce nanoparticles. The bacterial production of nanoparticles as a green and one-step method can overcome production problems and disadvantages through physical and chemical methods. Materials and Methods: In this study, bacteria isolates were taken from soil samples of Nakhlak mine. One silver resistant bacterial strain was selected for the production of silver nanoparticles. This strain synthesized silver nanoparticles with supernatant and biomass. The nanoparticles synthesized were characterized using XRD and SEM. The antimicrobial activity of the nanoparticles was determined. Results: The silver resistance bacteria were isolated from soil samples. One silver resistant bacterial strain was selected for the production of silver nanoparticles. The nanoparticles showed good antimicrobial activity against standard strains of tested microorganisms. Conclusion: The nanoparticle synthesized by bacterial strain isolated from Nakhlak mine is a promising new biological source for synthesizing silver nanoparticles with potent antimicrobial activity. According to the results, this compound can be used to make disinfectants
Biotechnology & nanotechnology
Akbar Safipour Afshar; fateme Saied Nematpour
Volume 27, Issue 4 , November and December 2020, , Pages 550-558
Abstract
Introduction: Green synthesis of silver nanoparticles is a simple and low-cost method. In addition, finding new therapeutic effects for silver nanoparticles is considered as an attractive field in cancer research. In the present study, synthesis of silver nanoparticles was performed by aqueous leaf extract ...
Read More
Introduction: Green synthesis of silver nanoparticles is a simple and low-cost method. In addition, finding new therapeutic effects for silver nanoparticles is considered as an attractive field in cancer research. In the present study, synthesis of silver nanoparticles was performed by aqueous leaf extract of barberry. The effects of Ag nanoparticles were evaluated on cell viability and expression levels of ODC1 gene of the human breast cancer cell line (MDA-MB-231).
Materials and Methods: The synthetic nanoparticles were analyzed by UV-vis spectrophotometry and Transmission Electron Microscopy. The cultured cancer cells were treated under different concentrations of synthesized nanoparticles.The viability of the cells was analyzed by MTT assay and relative expression of ODC1 gene by Real-Time PCR.
Results: TEM photomicrographs showed that the spherical silver nanoparticles have been synthesized with an average size of 16 nm. The results showed cytotoxicity of silver nanoparticles in a time and dose-dependent manner. The average IC50 calculated was 16.48 µg/ml. The results also showed that silver nanoparticles decreased ODC1 gene expression levels about 4 folds at 20 µg/ml nanoparticles compared to control.
Conclusion: It seems that green synthesized silver nanoparticles through the gradual release of silver ions in the acidic environment of the cancer cells and production of oxygen radicals, as well as reduction of ODC1gene expression cause diminish of cancer cell viability.