immunology & Biochemistry
mahsa saeedpour; mohamad fazilati; zolfaghar lotfi
Abstract
Introduction: The COVID-19 pandemic, caused by the coronavirus, has emerged as one of the most pressing healthcare challenges of this century. The role of laboratory data in diagnosing severe forms of COVID-19 remains inconclusive. This research aimed to find ways to quickly prevent COVID-19.Materials ...
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Introduction: The COVID-19 pandemic, caused by the coronavirus, has emerged as one of the most pressing healthcare challenges of this century. The role of laboratory data in diagnosing severe forms of COVID-19 remains inconclusive. This research aimed to find ways to quickly prevent COVID-19.Materials and Methods: This research involved 43 COVID-19 patients monitored from March 2022 to May 2022 at the Mehregan Private Center in Khuzestan Province. Hematological and biochemical tests of the patients were performed. Measurements of D-dimer and Interleukin-6 were performed using the Biotek ELX 800TS analyzer, while fibrinogen levels were determined through the Clauss coagulation method. Data analysis was carried out using SPSS version 22 and GraphPad Prism version 9.Results: There is a significant difference in the levels of interleukin 6, D-Dimer, and fibrinogen, which was equal to 0.0001 (P<0.05). Threshold level and area under the receptor operating characteristic curve (ROC) interleukin-6 was 20.06 pg/ml and 0.837, D-dimer 952.5 ng/ml and 0.929, and fibrinogen was 206.5 mg/dL and 0.768, respectively. The specificity and sensitivity of predicting the severity of COVID-19 interleukin 6 were 67.44% and 100%, D-dimer 60.47% and 97.67%, and fibrinogen 53.49 % and 95.35%, respectively. Interleukin-6 and D-dimer had the highest specificity and sensitivity for early prediction of the severity of COVID-19, but for fibrinogen, this level was not as obvious as the other two factors.Conclusion: D-dimer and interleukin-6 had more pronounced effects on the severity of this disease, while fibrinogen had a lower impact.
Physiology & Pharmacology
Bahram Farhadi Moghaddam; Masoud Fereidoni
Volume 28, Issue 1 , January and February 2021, , Pages 116-122
Abstract
Introduction: Many investigations revealed that the inflammatory process induced by cerebral ischemia/reperfusion causes brain damages and cognitive impairments. On the other hand, Menaquinone-4 (MK-4) is one of the important vitamin K2 types that has anti-inflammatory effects. Therefore, in this study, ...
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Introduction: Many investigations revealed that the inflammatory process induced by cerebral ischemia/reperfusion causes brain damages and cognitive impairments. On the other hand, Menaquinone-4 (MK-4) is one of the important vitamin K2 types that has anti-inflammatory effects. Therefore, in this study, we investigated the effect of administration of MK-4 on the level of gene expression of proinflammatory cytokines following global ischemia/reperfusion in the hippocampus of male Wistar rats. Materials and Methods: In this research, 20 adult male Wistar rats (250-300 g) were randomly selected in 5 experimental groups and studied: control (intact), sham (surgery without carotid artery occlusion), ischemia/reperfusion, ischemia/reperfusion + intraperitoneal (i.p.) injection of DMSO as MK-4 solvent, treatment (ischemia/reperfusion + i.p. injection of MK-4). For induction ischemic model, common carotid occlusion was performed for 20 minutes. In the treatment group i.p. injection of 200 mg/kg MK-4 was done 20 minutes after obstruction (immediately and 2 hours after reperfusion). 24 hours after reperfusion, mRNA expression level of TNF-α, IL-1β and IL-6 were assessed. Results: I.p. administration of MK-4 could significantly decrease mRNA expression level of TNF-α (p < 1.15), IL-1β and IL-6 (p < 0.001) induced by ischemia/reperfusion. Conclusion: The findings of this study show that MK-4 administration following cerebral ischemia/reperfusion could diminish the expression of the pro-inflammatory factors in the hippocampus and maybe cause neuroprotective effects. Received.
Mokhtar Nasirifarsani; Mona Sarhadi; Farzaneh Rahmani; Kiana Hasanzadeh
Volume 24, Issue 5 , September and October 2018, , Pages 339-345
Abstract
Background: In previous studies, pro-inflammatory and anti-inflammatory cytokines were shown to increase after physical activity. The purpose of this study was to examine the effects of different carbohydrate-protein supplementation ratio on plasma levels of IL-6 and IL-10 after high-intensity exercise ...
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Background: In previous studies, pro-inflammatory and anti-inflammatory cytokines were shown to increase after physical activity. The purpose of this study was to examine the effects of different carbohydrate-protein supplementation ratio on plasma levels of IL-6 and IL-10 after high-intensity exercise in healthy young females. Materials and Methods: Twenty seven volunteer females aged 20-25 year divided into three groups: 1-placebo, 2- carbohydrate-protein (3:1), and carbohydrate-protein (4:1). After 12 h fasting, subjects have standard breakfast and then completed anaerobic test at 10 AM. Subjects received one of supplementations pre-, post-, and 2 h after test. Blood samples were taken before, immediately and 2 h after exercise. Data were analyzed by MANOVA and repeated measure ANOVA. Statistical significance was accepted at the 0.05 level. Results: IL-6 and IL-10 increased significantly after test and then 2 hours after test IL-6 decreased and IL-10 remained high in all groups. IL-10 and IL-6 cytokines didn’t differ between groups. Although, IL-10 was higher and IL-6 was lower in carbohydrate-protein (4:1) group than placebo group at two hours after exercise but those were not statistically significant. Conclusion: carbohydrate-protein (4:1 ratio) supplementation resulted to some extent in improvements in cytokine responses at 2 hours after high-intensity exercise test.