Physiology & Pharmacology
Soodabeh Elyasi; Jina Khayatzadeh; saeedeh zafarbalanejad; Sepideh Elyasi; mohammad ALIZADE
Volume 28, Issue 5 , November and December 2021, , Pages 675-687
Abstract
Introduction: Fibroblasts are involved in production of growth factors which are effective on cells’ growth and differentiation. They are the most adaptable cells in connective tissue with significant capacity for differentiation to the other cell group. Insulin-like growth factor-1 (IGF-1) plays ...
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Introduction: Fibroblasts are involved in production of growth factors which are effective on cells’ growth and differentiation. They are the most adaptable cells in connective tissue with significant capacity for differentiation to the other cell group. Insulin-like growth factor-1 (IGF-1) plays an important role in differentiation and growth of different cell lines. Silibinin is extracted from seeds of Silybum marianum, which it's effects in cancer cell lines, have been studied in limited studies. In this study we evaluated the silibinin effect on viability and IGF-1 gene expression in human foreskin fibroblast (HFF).Materials and Methods: The cytotoxic effect of 10, 20, 40 & 60 µM solution of silibinin was evaluated on HFF cells using MTT assay, after 24 & 48 hours. Then, the expression of IGF-1 gene was evaluated by means of real time-PCR.Results: Silibinin had toxic effect on HFF cells in dose-dependent manner after 24 hours of incubation in comparison with control group but no significant difference observed after 48 hours. Besides, after 24 hours of incubation, silibinin with a concentration of 20-40-60 µM significantly increased the IGF-1 gene expression in fibroblast cells in comparison with control group.Conclusion: Based on the results, silibinin significantly induces IGF-1 gene expression in dose dependent manner after 24 hours incubation with HFF cells. However, in cytotoxicity assay, concentration of 60 µM caused the highest rate of cell death after 24 hours. So, before introduction of this compound as a fibroblasts proliferation stimulant, more extensive studies are needed.
Sayedeh Elmira Yazdi-Rouholamini; Nasrin Motamed; Mohammad Tahmasb; Kobra Omidfar
Volume 23, Issue 3 , July and August 2016, , Pages 386-391
Abstract
Background & Objectives: Metastatic cancers, such as breast cancer, are generally resistant to chemotherapy. Therefore many researches try to find new and effective compounds to treat this type of cancer. In this study, we examined the Silibinin toxic effect on tumor growth and proliferation of MCF-7 ...
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Background & Objectives: Metastatic cancers, such as breast cancer, are generally resistant to chemotherapy. Therefore many researches try to find new and effective compounds to treat this type of cancer. In this study, we examined the Silibinin toxic effect on tumor growth and proliferation of MCF-7 cell line. Materials & methods MTT assay was used to assess the inhibitory effect of silibinin on metabolic activity of MCF_7 cells. For this purpose, seeded cells were plated on to 96-well plates and then exposed to varying concentrations of silibinin (0-350 ug/mL) for different time (24, 48 and 72 h). The percentage of metabolic activity was calculated according to the MTT test. In next step, in order to evaluate the effect of silibinin on apoptosis in MCF_7 cells, Caspase 3/7 test was used. Results: Our results indicate that silibinin inhibited metabolic activity of MCF-7cells in a dose-dependent manner and it can induce apoptosis pathway in these cells. Conclusion: The study showed that treatment of MCF-7 cell line with Silibinin can be through induction of apoptosis, inhabitation of the growth and proliferation of cells. Due to the significant inhibitory effect of silibinin on MCF-7 cells, it seems important for researchers to utilize milk thistle in the treatment and prevention of cancer.