Fatemeh Masomi; Mehdi Hassanshahian; Mahasti mohammadi
Volume 25, Issue 4 , September and October 2018, , Pages 498-507
Abstract
Background and aim: In this study the antimicrobial effect of alcoholic extracts of Piper nigrum was evaluated against six antibiotic resistance pathogenic bacteria. Material and methods: The ethanolic extracts was prepared by modified maceration method. The antibacterial effects of extracts was assayed ...
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Background and aim: In this study the antimicrobial effect of alcoholic extracts of Piper nigrum was evaluated against six antibiotic resistance pathogenic bacteria. Material and methods: The ethanolic extracts was prepared by modified maceration method. The antibacterial effects of extracts was assayed against six antibiotic resistant bacteria by disc diffusion method. Also, the minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of each extracts was determined by macro-broth dilution method. The antibacterial effect of these extracts was analyzed on biofilm formation, destruction and inhibition of enzyme activity of bacteria. Results: In disc diffusion assay the maximum and minimum inhibitory effects of extracts was related to S. aureus and E. coli respectively. The methanolic extract with maximum MIC (50 mg/ml) had the lowest effect against P. aeruginosa. This extract with minimum MIC (25 mg/ml) had the maximum inhibition against E. coli. The maximum and minimum inhibitory effect on biofilm formation related to methanolic and ethanolic extracts at concentration (25 mg/ml) against S. aureus. The highest destruction of biofilm structures was observed in treatment of ethanolic extract against E. coli and the lowest destruction of biofilm was recorded for Acinetobacter. The maximum enzyme inhibition activity was observed in treatment with methanolic extract against Acinetobacter and the minimum effect of enzyme inhibition activity recorded for ethanolic extract against Klebsiella.Conclusion: Medical application of extracts and essential oil of medicinal plants for low side effects is valuable.
Seyyed Kazem Sabbagh; Saeedeh Saeedi; Zahra Dehbashi; Mahta Mazaheri Naeieni
Volume 22, Issue 5 , November and December 2015, , Pages 854-861
Abstract
Background and aim: Staphylococcus aureus is on of the most infectious agents to humans. In recent years, resistance strain to different antibiotic has been reported. With increasing of bacterial resistance to chemical drugs and effects of plant medicines, herbal medicines have been considered as alternative ...
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Background and aim: Staphylococcus aureus is on of the most infectious agents to humans. In recent years, resistance strain to different antibiotic has been reported. With increasing of bacterial resistance to chemical drugs and effects of plant medicines, herbal medicines have been considered as alternative medicaments. The purpose of this study was to investigate the antimicrobial activity of alcoholic extract of Black pepper and March against clinical isolates of Staphylococcus aureus resistant to commonly used antibiotics.
Materials and Methods: Alcholic plant extraction was done using a rotary apparatus. Twelve, Staphylococcus aureus strains from urine samples was isolated and then purified. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBI) of the plant extracts at six different concentrations was determined broth medium using dilution method. Susceptibility of strains to multiple antibiotics was evaluated using Kirby-Bauer disk diffusion method.
Result: The results of this study showed that higher and lower average resistance to tested antibiotic is belonged to Ceftazidime (66/66%) and vancomycin (8/1%) antibiotic, respectively. MIC assay of plant extracts showed that the highest effect of Pepper and March was determined at concentrations of 10 and 5 mg/ml, respectively. Whereas, the lowest MIC effect for Pepper and March was recorded at concentration of 2/5 and 0/62 mg/mL, respectively. The maximum MBC of plant extracts was recorded at concentration of 20 and 10 mg/mL for Pepper and March, respectively.
Conclusion: Although the clinical application of plant extracts and essential oils due to fewer side effects, seems valuable, However, to clinical application of the alcoholic extract of black pepper and march more researches on mode of action of effective compounds of plans on microbial agents have to be done.