Shahram Nazari; Sosan Bagheri; Lila Mohammadi; Gharib Majidi; Payman Azghani; Zohreh Darkhosh; Zohreh Nazari; Soudabeh Alizadeh Matboo
Volume 24, Issue 6 , March and April 2018, , Pages 112-120
Abstract
Background & Objectives: This study aimed to examine the antibacterial effect of Nano Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus isolated from the hospital environment. Materials & Methods:In this ...
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Background & Objectives: This study aimed to examine the antibacterial effect of Nano Polyamidoamine-G5 (NPAMAM-G5) dendrimer on Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus isolated from the hospital environment. Materials & Methods:In this study, the antibacterial effects of NPAMAM-G5 dendrimer were studied by disc diffusion and micro-dilution method. Different concentrations of Polyamidoamine-G5 inoculated onto blank disks and were placed in Mueller-Hinton agar media. Zone of inhibition was investigated by bacterial inoculation according to the McFarland standard 0.5. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of NPAMAM-G5 dendrimer were determined by micro-dilution method. Results: Zone of inhibition in Polyamidoamine-G5 dendrimers concentration of 25 μg/mL for Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus were 22, 28, 21, 16 and 20 mm, respectively. Regarding the zone of inhibition in gram negative bacteria with gram positive ones was p=0.16 and was not significant different. The minimum inhibitory concentration for both Proteus Mirabilis and Salmonella Typhi was 2.5 μg/mL and for Escherichia Coli, Bacillus Subtilis and Staphylococcus Aureus was 25 μg/mL. The Minimum Bactericidal Concentration (MBC) for Salmonella Typhi was 50 μg/mL and for all Proteus Mirabilis, Escherichia Coli, Bacillus Subtilis and Staphylococcus Aureus was 100 μg/mL. Conclusion: According to the results, NPAMAM-G5 dendrimer can effectively eliminate Escherichia Coli, Proteus Mirabilis, Salmonella Typhi, Bacillus Subtilis and Staphylococcus Aureus.
Seyyed Kazem Sabbagh; Saeedeh Saeedi; Zahra Dehbashi; Mahta Mazaheri Naeieni
Volume 22, Issue 5 , November and December 2015, , Pages 854-861
Abstract
Background and aim: Staphylococcus aureus is on of the most infectious agents to humans. In recent years, resistance strain to different antibiotic has been reported. With increasing of bacterial resistance to chemical drugs and effects of plant medicines, herbal medicines have been considered as alternative ...
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Background and aim: Staphylococcus aureus is on of the most infectious agents to humans. In recent years, resistance strain to different antibiotic has been reported. With increasing of bacterial resistance to chemical drugs and effects of plant medicines, herbal medicines have been considered as alternative medicaments. The purpose of this study was to investigate the antimicrobial activity of alcoholic extract of Black pepper and March against clinical isolates of Staphylococcus aureus resistant to commonly used antibiotics.
Materials and Methods: Alcholic plant extraction was done using a rotary apparatus. Twelve, Staphylococcus aureus strains from urine samples was isolated and then purified. The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBI) of the plant extracts at six different concentrations was determined broth medium using dilution method. Susceptibility of strains to multiple antibiotics was evaluated using Kirby-Bauer disk diffusion method.
Result: The results of this study showed that higher and lower average resistance to tested antibiotic is belonged to Ceftazidime (66/66%) and vancomycin (8/1%) antibiotic, respectively. MIC assay of plant extracts showed that the highest effect of Pepper and March was determined at concentrations of 10 and 5 mg/ml, respectively. Whereas, the lowest MIC effect for Pepper and March was recorded at concentration of 2/5 and 0/62 mg/mL, respectively. The maximum MBC of plant extracts was recorded at concentration of 20 and 10 mg/mL for Pepper and March, respectively.
Conclusion: Although the clinical application of plant extracts and essential oils due to fewer side effects, seems valuable, However, to clinical application of the alcoholic extract of black pepper and march more researches on mode of action of effective compounds of plans on microbial agents have to be done.