Microbiology
Morteza Yazdani; Fereshteh Jookar Kashi; Akram Rahimi-Moghaddam
Volume 26, Issue 5 , November and December 2019, , Pages 555-565
Abstract
Background: Considering the adverse effects of synthetic antioxidants, anticancer drugs and antibiotics on human health, in this study, bioactivities of Mentha longifolia essential oils collected from Marivan and Qaza An were examined and compared with each other for identification a natural alternative ...
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Background: Considering the adverse effects of synthetic antioxidants, anticancer drugs and antibiotics on human health, in this study, bioactivities of Mentha longifolia essential oils collected from Marivan and Qaza An were examined and compared with each other for identification a natural alternative of synthetic antioxidants, anticancer drugs and antibiotics.Materials and Methods: Essential oil of M. longifolia leaves and stems collected from Marivan and Qaza An was prepared using solvent-free microwave extraction method. The essential oil components were identified by gas chromatography/mass spectrometry. The antioxidant and cytotoxicity activity of the essential oils were determined via β–carotene bleaching assay and brine shrimp lethality test, respectively. The antimicrobial activity of the essential oils was evaluated by the agar well diffusion method and by determination of minimum inhibitory concentration.Results: The main components of M. longifolia essential oil collected from Marivan were pulegone (81.45%) and isopulegol (8.39%) and the main components of M. longifolia essential oil collected from Qaza An were pulegone (48.29%) and piperitenone oxide (23.53%). The inhibition of linoleic acid oxidation by M. longifolia essential oils collected from Marivan, Qaza An and BHT were 80.02%, 78.59% and 95.50%, respectively. The LC50 of the M. longifolia essential oils collected from Marivan, Qaza An and vincristine sulfate were 53.47μg/ml, 55.96 μg/ml and 0.751 μg/ml, respectively. M. longifolia essential oils had good antimicrobial activity, especially against gram-positive bacteria.Conclusion: M. longifolia essential oils have significant antioxidant, antimicrobial and anticancer activity and can be introduced as alternatives of synthetic antioxidants, antibiotics and anti-cancer drugs.
Omid Azizian Shermeh; Mozhgan Taherizadeh; Moharam Valizadeh; Alireza Zaboli
Volume 25, Issue 2 , May and June 2018, , Pages 279-292
Abstract
Background: In this study antimicrobial activities, antioxidant activities and phytochemical compounds in the essential oil and extracts of Cumbopogon olivieri were studied.Material & Methods: For investigation of phytochemical compounds such as total phenolic and total flavonoid contents in three ...
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Background: In this study antimicrobial activities, antioxidant activities and phytochemical compounds in the essential oil and extracts of Cumbopogon olivieri were studied.Material & Methods: For investigation of phytochemical compounds such as total phenolic and total flavonoid contents in three extracts (Methanolic, ethanolic and aqueous) and essential oil by Folin-Ciocaltiu, Aluminum Chloride colorimetric and GC-MS methods and antioxidant activities of extracts by two methods (DPPH and FRAP) and antimicrobial activities of essential oil and extracts of Cumbopogon olivieri against three bacteria such as Staphylococcus aureus, Bacillus cereus, Escherichia coli and two fungi such as Candida albicans and Aspergillus niger by two methods (well diffusion and Minimal Inhibitory Concentration (MIC)) respectively.Results: The results showed that, the methanolic extract had maximum total phenolic and flavonoid contents and antioxidant activity and aqueous extract had minimum value of this compounds and activity. In investigation of essential oil, three compounds such as piperitone (82.95%), carene (2.493%) and limonene were the major constituents. The results of antimicrobial activity showed that, the essential oil and extracts had good inhibition effect on all of bacteria and fungi and this inhibition depends to concentration.Conclusion: Overall, based on the results obtained, the plant can be a good candidate for the treatment of diseases caused by oxidative stress, and diseases caused by pathogenic microbes. Also due to power up the plant in the elimination of pathogens, could be a viable alternative to antibiotics.
Masood Homayoni Tabrizi; Ahmad Asoodeh; Hoda Shabestarian
Volume 22, Issue 1 , March and April 2015, , Pages 45-56
Abstract
Background & objective: Due to the side effects of synthetic antioxidants, bioactive agents derived from natural sources are of great importance. The purpose of this study was to identify and characterize an antioxidant peptide derived from enzymatic hydrolysis of β-casein in camel milk, using ...
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Background & objective: Due to the side effects of synthetic antioxidants, bioactive agents derived from natural sources are of great importance. The purpose of this study was to identify and characterize an antioxidant peptide derived from enzymatic hydrolysis of β-casein in camel milk, using pepsin and pancreatin. Materials and Methods: Enzymatic hydrolysis of camel milk had accomplished using pepsin and pancreatin. The hydrolysate was fractioned using RP-HPLC and the peptide of interest was identified with MALD-TOF/TOF technique. Antioxidant activity of isolated peptide was measured by the use of radical scavenging DPPH, ABTS, hydroxyl and superoxide and inhibition of linoleic acid oxidation. Results: The results of sequencing showed that a purified peptide, called RQ-8, has the sequence of RGLHPVPQ with molecular weight of 903.41 Da. This peptide inhibited oxidation of linoleic acid and also had scavenging activity against 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC50=0.046 mg/ml), 2,2'-azinobis (3-ethylbenzothiazo-line-6-sulfonic acid) diammonium salt (ABTS) (IC50 = 0.085 mg/ml), superoxide (O2·-) (IC50 = 0.156 mg/ml) and hydroxyl (OH ·-) (IC50 = 0.021 mg/ml) radicals. In addition, cellular activity assays showed that the RQ-8 peptide had no toxicity on A549 lung cancer cell line. Conclusion: Results indicated that the peptide RQ-8 isolated from camel milk β-casein protein has a strong antioxidant activity.