Maryam Noorshahi; Samaneh Koneshloo; Mehdi Hedayati; Mostafa Baranchi; Nafiseh AminolEslam; Javad Nemati
Volume 21, Issue 6 , January and February 2015, , Pages 1113-1122
Abstract
Background and Purpose: Mechanical tension induced by resistance exercise can stimulate tension-sensitive cytoskeletal proteins. Also, resistance exercise can induce inflammatory factors. Therefore, the aim of this study was to investigate the changes of inflammatory and hypertrophic markers and their ...
Read More
Background and Purpose: Mechanical tension induced by resistance exercise can stimulate tension-sensitive cytoskeletal proteins. Also, resistance exercise can induce inflammatory factors. Therefore, the aim of this study was to investigate the changes of inflammatory and hypertrophic markers and their association with a single bout of resistance exercise in male rats.
Materials and Methods: Twelve male Wistar rats, three months of age, were randomly assigned into control and experimental groups. Resistance exercise protocol (to climb up a one-meter ladder, 3 sets, 10 repetitions in each set, at 50%, 75% and 100% of their own body weight) was performed. The control group did not perform any exercise activity. Twelve hours after the last session of exercise, rats (both control and experimental) were anesthetized with a mixture of ketamine/xylazine. Blood samples were taken directly from the heart; their Soleus muscles were extracted. Vinculin levels in muscle tissue after homogenation and IL-17 levels in sera were measured using ELISA method. Creatine kinase levels were measured by enzymatic colorimetric methods. Independent t-test and Pearson correlation coefficient were used. A significance level of α= 0.05 was considered.
Results: There was no significant diferences between control and experimental groups in vinculin, IL-17 and creatine kinase levels (p>0.05). Nevertheless, there was a significant negative corrolation between vinculin and IL-17 (r=-0.93, p=0.01).
Conclusion: A single bout of resistance training can lead to a change in vinculin, IL-17 and creatine kinase levels. Interaction between inflammatory and hypertrophic markers (especially vinculin and IL-17) probably has a role in adaptations induced by resistance training.
Bahram RashidKhani; Tirang Rezaneyestani; Shahin Rasooli; Atena Ramezani; Farideh Tahbaz
Volume 17, Issue 4 , January and February 2011, , Pages 236-247
Abstract
Background and Purpose: Variation and increase in the levels of inflammatory factors and glycemia are among the major problems of type 2 diabetics. Therefore, the present study was designed to investigate the effect of beta carotene-enriched carrot juice on CRP inflammatory indexes, IL-6 and FBS glucose ...
Read More
Background and Purpose: Variation and increase in the levels of inflammatory factors and glycemia are among the major problems of type 2 diabetics. Therefore, the present study was designed to investigate the effect of beta carotene-enriched carrot juice on CRP inflammatory indexes, IL-6 and FBS glucose in diabetics. Methods and Materials: This randomized double blind clinical trial was conducted on 44 type 2 diabetics. Participants based on gender and BMI were assigned into two groups (Group A receiving 200 ml carrot juice enriched with 10 mg beta carotene and Group B receiving normal carrot juice) by randomized stratified sampling. Both groups received 200 ml carrot juice for 8 weeks for lunch (instead of cereals). A 24-hour recall was obtained for 3 consecutive and 6 concomitant days at the beginning and end of the study duration. Also, CRP, IL-6, FBS glucose and serum beta carotene levels were measured in FBS blood sample at the beginning and end of the study duration. The consumption data were fed into Nutritionist IV, and analyzed in SPSS 11.5. Results: Each group consisted of 22 (11 male and 11 female diabetics) participants. There was a significant increase in the levels of beta carotene in group A (111.9±64.9 at the beginning and 72±43.9 at the end) (p=0.02). However, the reduction of CRP in Group A (-996.9±2172.6 at the beginning and -537.3±1412.4 at the end) was not significant (p=0.098). Also, IL-6 reduction in Group A (-0.8±2.1 at the beginning and -0.6±2.4 at the end) was not significant (p=0.085). FBS glucose was also not examined in the present study. Conclusion: Daily consumption of carrot juice enriched with 10 mg beta carotene for eight weeks caused an improvement in the levels of serum antioxidants including beta carotene in type 2 diabetics, with no significant effects on serum glucose and inflammatory indexes.