Physiology & Pharmacology
Mahmoud Alipour; Farkhondeh Nemati
Volume 26, Issue 1 , March and April 2019, , Pages 145-152
Abstract
In the last few decades, research has been focused on the use of natural products for cancer therapy, such as crude plant extracts or some of different plant secondary metabolits. The objective of this study was to examine the invitro cytotoxic activities of crude ethanolic extract of leaf and flower ...
Read More
In the last few decades, research has been focused on the use of natural products for cancer therapy, such as crude plant extracts or some of different plant secondary metabolits. The objective of this study was to examine the invitro cytotoxic activities of crude ethanolic extract of leaf and flower buds of Crataegus melanocarpa, on human MCF-7 and HeLa cell lines, with different concentrations, by Dye exclusion and Micro culture tetrazolium test (MTT) assay. The optical density (OD) colored solution was quantified at 570 nm wavelengths by an ELISA Reader, after 24, 48 and 72 h incubation. The results showed that ethanolic extracts, after 48h incubation, at in 5 and 10 mg/ml suppressed the proliferation of cancerous MCF-7 cells by significant diffrence with control group (p
Ali Asghar Ghaderi; Baratali Fakheri; Nafise Mahdinezhad; Saeede Saeedi
Abstract
Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus ...
Read More
Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus pyogenes ATCC 19615, Streptococcus pneumoniae ATCC 49619, S. saprophyticus ATCC15305, Hafnia alvei ATCC 51873, Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratia marcescens ATCC 274, Staphylococcus aureus ATCC 25923.The minimum inhibitory concentration (MIC) of thymus ethanol extract on the bacteria was determined. The linear sensitivity of the bacteria against some antibiotics was also measured by the standard method of Kirby-Bauer disk diffusion. Results The results showed that the thymus leaf alcoholic extract inhibited the bacterial growth in the evaluated concentrations. The MIC of 6/25 ppm was observed effective against S. pneumoniae, H. alvei, P. mirabilis, and S. marcescens.Conclusion The results of the current study indicated the high effect of thymus alcoholic extract on the pathogenic bacteria. However, the medicinal application of plants requires further investigations.
Ali Salehi; Nabi Shariatifar; Aftab Salehi; Alireza Mohammadzadeh
Volume 23, Issue 3 , July and August 2016, , Pages 430-437
Abstract
Background Food-borne pathogens are a group of microorganisms that cause food-borne illness, and the research for finding effective natural products against the infection arisen from these microorganisms is necessary. The purpose of this study was to determine antimicrobial effect of aqueous and ethanol ...
Read More
Background Food-borne pathogens are a group of microorganisms that cause food-borne illness, and the research for finding effective natural products against the infection arisen from these microorganisms is necessary. The purpose of this study was to determine antimicrobial effect of aqueous and ethanol extracts of Rheum ribes on some important foodborne bacteria.Materials & Methods After collecting the leaves and stalks of rhubarb in the spring, its different concentrations of 50, 75, 100, 125, and 150 mg/mL were prepared. Then, antibacterial effect of aqueous and ethanol extracts were determined by the disk diffusion and serial dilution methods.Results Findings showed that aqueous and ethanolic extracts of stalks and leaves of rhubarb have good inhibitory effect on S. aureus and E. coli. The minimum inhibitory concentration (MIC) of the ethanolic leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤75, ≤50, ≤100, and ≤75, respectively, and MIC of the aqueous leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤100, ≤100, ≤125, and ≤100, respectively. The minimum bactericidal concentration of the ethanolic leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤100, ≤75, ≤150, and ≤125, respectively, and aqueous extracts did not have bactericidal effects.Conclusion Extracts of rhubarb leave and stalk showed a good antimicrobial activity against food-borne pathogens. Therefore, they can be used in food preservation systems to inhibit the growth of these bacteria and improve food quality and safety.
Mohammad Bokaeian; Faramarz Dahmardeh; Mohammad Sohrabi; Saeide Saeidi
Volume 22, Issue 5 , November and December 2015, , Pages 780-787
Abstract
Background: Infectious diseases are an important cause of mortality in the world. Because of resistance of some human pathogenic bacteria to antibiotics, use of natural and herbal medicine is increasing to control these factors. The aim of this study is to investigate the antibacterial effects of Ramalina ...
Read More
Background: Infectious diseases are an important cause of mortality in the world. Because of resistance of some human pathogenic bacteria to antibiotics, use of natural and herbal medicine is increasing to control these factors. The aim of this study is to investigate the antibacterial effects of Ramalina hyrcana Sipman lichens against many Gram-positive and Gram-negative bacteria.
Materials and Methods: The lichen ethanol extract was prepared by using a rotary machine. Nine standard strains of Gram- positive and Gram -negative bacteria were cultured in nutrient broth. The minimum inhibitory concentration (MIC) was determined by the micro dilution method.
Results: The results of this study showed that the lichen R. hyrcana extract in different concentrations inhibited both Gram-positive and Gram-negative bacteria. The results showed that the lichen extract of all concentrations inhibited of Staphylococcus aureus. After S. aureus bacteria inhibition, the minimum inhibitory concentrations are for Hafnia and Acinetobacter (12.5 ppm).
Conclusion: According to the results and other reports in this area, lichen R. hyrcana extract could be introduced as a biological control agent. However, for the use of different lichen extracts for clinical application, the chemical analysis of extracts and clinical research is necessary.
Leylasaadat Aldaghi; Abbasali DehpoorJoybari; Farkhondeh Nemati; Reza Mirdashti; Rahim Akrami
Volume 20, Issue 5 , March and April 2014, , Pages 603-610
Abstract
Abstract
Background & objectives: Cancer prevention and treatment by medicinal plants for a long was is one of the most challenging areas of research. Among them, some plants species could suppress or kill tumor cells via apoptosis or necrosis. One of these plants is Astragalus. The purpose of this ...
Read More
Abstract
Background & objectives: Cancer prevention and treatment by medicinal plants for a long was is one of the most challenging areas of research. Among them, some plants species could suppress or kill tumor cells via apoptosis or necrosis. One of these plants is Astragalus. The purpose of this study was investigating the anti-cancer effects of this plant.
Material & method: The cancer cell lines obtained from Pasteur Institute of Iran. The cells were cultured in RPMI 1640. Then the cells have effected from the presence of various concentrations (0.156, 0.312, 0.625, 1.25, 2.5, 5, 7.5 and 10 mg/ml) of Astragalus cystosus. After 72 hours the rate of cytotoxicity was determined by using MTT test. These statistical analyses carried out with a significant level of 0.05 and t-test with excel software.
Findings: These findings indicate that the extract of Astragalus cystosus on Hela cancer cell lines had cytotoxicity in all concentrations and the highest inhibition was 7.5 and 10 mg/ml concentrations. The rate of inhibition in higher concentrations such as 10mg/m1 was equal to 28.98% which is the highest one.
Conclusion: Regarding that the extract of Astragalus cystosus had cytotoxicity on hela cancer cells, further studies can be performed on animal model and clinical trial so that Astragalus used as an anticancer drug.