Microbiology
haniyeh bashi fakhar; Foroogh Eshaghi kojor; Masoud Ghane; javad Shokri
Volume 29, Issue 5 , November and December 2022, , Pages 655-668
Abstract
Introduction: Streptococcus galloliticus is one of the bacteria associated with colorectal cancer in humans. No studies have been performed to identify this bacterium in the large intestine using PCR test and compare it with microbial culture in patients undergoing colonoscopy in northern Iran.
Materials ...
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Introduction: Streptococcus galloliticus is one of the bacteria associated with colorectal cancer in humans. No studies have been performed to identify this bacterium in the large intestine using PCR test and compare it with microbial culture in patients undergoing colonoscopy in northern Iran.
Materials and Methods: In a descriptive study to diagnose Streptococcus gallolyticus, biopsy specimens were obtained from 55 individuals undergoing colonoscopy referred to Babol and Chalous hospitals. To detect bacteria after DNA extraction, first designed primers (PCO3, PCO4) were used to qualitatively analyze the extracted DNA and then the specific gene of Streptococcus gallolyticus was amplified. In addition to culture, diagnostic tests such as gram staining, catalase test, hydrolase, hydrate hydrolysis and scolin hydrolysis were used.
Results: In this descriptive study, out of 55 biopsy specimens of individuals undergoing colonoscopy, 3 specimens (5.5%) with 95% confidence interval were positive and 52 cases (5.94%) were negative for Streptococcus gallolyticus DNA. There was a significant relationship between the two diagnostic methods of culture and PCR (p.value 0.015).
Conclusion: The simultaneous application of the two methods is recommended in cases where the result is rapid.
Reza Ghotaslou; Tahereh pirzadeh; Aylin Esmailkhani; Abed Zahedi Bialvaie; Hamed Ebrahimzadeh Leylabadlo
Volume 29, Issue 2 , July and August 2022, , Pages 179-194
Abstract
Helicobacter pylori is the first formally recognized bacterial carcinogen and is one of the most effective human pathogens, as over half of the world's population is colonized with this Gram-negative bacterium. Unless treated, colonization usually persists lifelong. H. pylori infection represents a key ...
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Helicobacter pylori is the first formally recognized bacterial carcinogen and is one of the most effective human pathogens, as over half of the world's population is colonized with this Gram-negative bacterium. Unless treated, colonization usually persists lifelong. H. pylori infection represents a key factor in the etiology of the various gastrointestinal diseases, ranging from chronic active gastritis without clinical symptoms to peptic ulceration, gastric adenocarcinoma, and gastric mucosa-associated lymphoid tissue lymphoma (MALT). This review concerns some of the most recent developments in diagnostic methods of H. pylori infection and it has been studied in different clinical conditions. Only highly accurate tests should be used in clinical practice, and the sensitivity and specificity of an adequate test should exceed 90%. The choice of tests should take all items among into account clinical circumstances, the likelihood ratio of positive and negative tests, the cost-effectiveness of the testing strategy, and the availability of the tests.
Yaser Tabaraee; Elaheh HaghGoshaee; Afsaneh Takbiri; MohammadAli Yaghobifar
Volume 20, Issue 2 , May and June 2013, , Pages 154-164
Abstract
Objectives: improving patient safety culture in healthcare organization is the first step for reducing medical errors and improvement of patient safety. This study was conducted to survey the patient safety culture based on staff point of views in Sabzevar hospitals.
Methods: This cross-sectional and ...
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Objectives: improving patient safety culture in healthcare organization is the first step for reducing medical errors and improvement of patient safety. This study was conducted to survey the patient safety culture based on staff point of views in Sabzevar hospitals.
Methods: This cross-sectional and descriptive study was conducted during July- September 2011, by using Hospital Survey Of Patient Safety Culture questionnaire (HSOPSC). 385 of clinical staff (physicians, nurses, midwives and stuff working in lab and radiology departments) as census were included in the study. The patient safety culture questionnaire was used for data collection.
Results: “teamwork within unit” and “organizational learning - continuous improvement” had the highest percentages of positive responses with respectively 73.65 ± 6.4 % and 69.16 ± 8.7 % whereas “nonpunitive response” to errors (13.03 ± 7.1%) and “staffing” ( 22.37 ± 6%) had the chosen lowest percentages of positive responses. 89% of staff noted that they have not reported any medical errors during past 12 months. Only 31% of staff reported the patient safety status in their units as excellent or very good.
Conclusion: Hospitals can attempt to create safety environment for patients by applying the strengths of their patient safety culture and removing the weaknesses. Therefore hospitals should eliminate the staff fear of blame for their mistakes and create an atmosphere of open communications and continuous learning.