Mohammad-Shafi Mojadadi; Najmeh Mahmoodabadi; Melika Lal-Kargar; Hossein Elyasi
Abstract
Objectives Despite vaccination, rubella is still considered as a major disease and a threat to global health. According to evidence, approximately 100 000 cases of congenital rubella syndrome (CRS) occur annually in the world. The current study aimed at determining the immune status of rubella among ...
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Objectives Despite vaccination, rubella is still considered as a major disease and a threat to global health. According to evidence, approximately 100 000 cases of congenital rubella syndrome (CRS) occur annually in the world. The current study aimed at determining the immune status of rubella among female students of Sabzevar University of Medical Sciences, Sabzever, Iran.Methods In the current cross sectional study, 143 female students filled out the questionnaires focusing demographic and epidemiological properties (residency, history of rubella vaccine, history of rubella infection among him/herself and the family members). Then, after obtaining the informed consent, blood samples were obtained from the students, and anti-rubella IgM and IgG were measured by the enzyme-linked immunosorbent assay (ELISA) method. Data were analyzed by SPSS version 16, using descriptive statistical methods and Chi-square test.Results According to the results of the current study, 45.8% of the participants were from Sabzevar and the rest were from other cities of Iran; 3% of the students had not received rubella vaccine. Results obtained from ELISA test showed that all serum samples were negative for rubella-specific IgM. Also, 99.3% of the students had acceptable titers (>10 IU/mL) for the rubella-specific IgG.Conclusion The current study showed that the rubella immune status was quite satisfactory among the female students of Sabzevar University of Medical Sciences.
M MAK VANDI; M FATAHI ABDIZADE; AR SAMARBAFZADEH
Volume 12, Issue 3 , September and October 2005, , Pages 28-33
Abstract
Background and Purpose: Type a influenza virus causes infection in man, avian and primates. It has recently been reported that avian H5N1 and H9N2 subtypes were transferred to man. The purpose of the study is, therefore, to investigate the probability of H9N2 transmission from poultry to people engaged ...
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Background and Purpose: Type a influenza virus causes infection in man, avian and primates. It has recently been reported that avian H5N1 and H9N2 subtypes were transferred to man. The purpose of the study is, therefore, to investigate the probability of H9N2 transmission from poultry to people engaged in poultry farming industries.
Methods and material: In this study, serological tests were carried out on 100 blood samples of contacted cases and special antibody of H9N2 subtype was measured by hemagglutination inhibition. Also, the sensitivity and specificity of the prepared ELISA test kit were measured in comparison with the standard method of hemagglutination inhibition. Out of 100 HI tests, 45 cases were normally selected and measured by ELISA.
Results: 66 percent of contacted cases had the specific H9N2 antibody. ELISA specificity and sensitivity, in comparison with HI, were 20% and 87.5% respectively.
Conclusion: The Presence of H9N2 antibody in the subject's serum suggest the prevalence of H9N2 virus among poultry, its transfer to people exposed and the possibility of their infection. Also, diagnosis influenza H9N2 antibody.
R GOLMOHAMMADI; E SHIRZADEH; R KHASHEEI
Volume 12, Issue 2 , July and August 2005, , Pages 20-25
Abstract
Background and Purpose: Rubella is a member of togaviridate viruses, with minor morbidity in children. Rubella infection during pregnancy causes various malformations including congenital cataract. Immunity against it is, therefore, important in girls before marriage and it is recommended in girls without ...
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Background and Purpose: Rubella is a member of togaviridate viruses, with minor morbidity in children. Rubella infection during pregnancy causes various malformations including congenital cataract. Immunity against it is, therefore, important in girls before marriage and it is recommended in girls without antirubella antibody in some countries. This study is intended to determine the anti-rubella immunity in premarriage girls in Sabzevar, Iran.
Methods and Materials: This descriptive-analytical study was conducted on 186 pre-marriage girls in Sabzevar, Iran to determine serological anti-rubella (IgG and IgM)using ELlSA. NOVUM DIAGNOSTICA Kit was used for tests and SPSS for data analysis.
Results: 172 girls (92.5%) were positive and 14 girls (7.5%) negative, Le. high risk group.152 girls (81.7%) already infected with rubella were now positive and 11 girls (5.9%) negative.
Conclusion: Subjects' age and IgG antibody did not show any significant relationships while a significant relationship was found between exposure to rubella and IgG (p