Physiology & Pharmacology
Najmea Farhadi; Davood Mehrabani; Seyed Ebrahim Hosseini; seyede sara hashemi
Volume 28, Issue 5 , November and December 2021, , Pages 814-825
Abstract
Introduction: Cannabis is psychoactive substance that is abused by millions of people the world. Due to the high consumption of this substance among young people of reproductive age, the present study was performed to investigate the effect of cannabis on the growth of endometrial mesenchymal stem cells(msc) ...
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Introduction: Cannabis is psychoactive substance that is abused by millions of people the world. Due to the high consumption of this substance among young people of reproductive age, the present study was performed to investigate the effect of cannabis on the growth of endometrial mesenchymal stem cells(msc) derived from rats.Materials and Methods: In this experimental study, msc were extracted from the endometrium of rats and after culturing and confirming the mesenchymal nature of the cells by flowcytometry and by expressingCD34 and CD90 and not expressingCD105 markers,in the third passage of cell culture, the effects of cannabis in concentration of100and1000ng/ml were calculated on the growth of these cells within1to8days and the results were analyzed using ANOVA and Tuki tests.Results: Cells isolated from the endometrium adhered to the floor of the cell culture flask24hours after transfer. The mesenchymal nature of these cells was confirmed by the expression of CD90, CD105and non-expression of CD34markers.The results ofcell counts also showed the growth of cells treated with cannabis until the third day of treatment similar to the control group. From the fourth day in the treatment group with a dose of100ng/ml cannabis increased significantly top<0.05and in the treatment group with a dose of1000ng/ml. Third, there was significant decrease compared to the control group and from the fourth day, significant increase was observed atp<0.05.Conclusion: The results showed that the cells isolated from the endometrium were of the msc, and that cannabis probably stimulated the growth of these cells through cannabinoid receptors.
Physiology & Pharmacology
Marziyeh Fallahi; Seyed Ebrahim Hosseini
Volume 27, Issue 3 , September and October 2020, , Pages 348-353
Abstract
Background & aim:Of different parts of Walnut plant in traditional diseases treatment such as diabetes are used. Given role of stresses in diabetes and chemical drugs’ side-effects in treating this disease, this study aimed to investigate alcoholic effects of walnut leaves extract on lipid ...
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Background & aim:Of different parts of Walnut plant in traditional diseases treatment such as diabetes are used. Given role of stresses in diabetes and chemical drugs’ side-effects in treating this disease, this study aimed to investigate alcoholic effects of walnut leaves extract on lipid profile in adult male rats’ diabetes with swimming stress was performed.methods: This experimental study on 50 male rats matured divided into 5 groups: control, diabetic, diabetic treated with walnut leaves (200mg/kg), diabetes with swimming stress in water 15°C, diabetic treated with swimming stress and walnut leaves was done. The end, after 21 days injecting, weighing, and phlebotomizing rats, triglycerides serum levels, total cholesterol, LDL and HDL were measured and data using SPSS-18 software, ANOVA and Tukey were analyzed, so the significant data difference P
Physiology & Pharmacology
Fatemea Seyf; Seyed Ebrahim Hosseini
Volume 26, Issue 1 , March and April 2019, , Pages 46-51
Abstract
Background:Memory and learning are one of the most complex brain functions and a mechanism for encoding, storing and recalling learned information. Due to the prevalence of learning disabilities, especially in older adults, the present study aimed to investigate the effect of hydroalcoholic extract of ...
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Background:Memory and learning are one of the most complex brain functions and a mechanism for encoding, storing and recalling learned information. Due to the prevalence of learning disabilities, especially in older adults, the present study aimed to investigate the effect of hydroalcoholic extract of chamomile on avoidance memory levels in rats. Materials and Methods:In this experimental study, 48 adult male rats were divided into 6 groups: control, control 1 (treatment with normal saline) and control 2 (Shuttle Box) and three experimental groups receiving 200,100, and 400 mg/kg hydroalcoholic extract of chamomile by intraperitoneal injections. All of them were prescribed for 15 days. In this study, the Shuttle Box was used to measure memory and learning. The latency of entering and the time of remaining in a dark chamber of the Shuttle Box were recorded. The results were analyzed by ANOVA and Tukey tests using the SPSS-22 software. Results:The significance level of data was considered as p≤0.05. The results showed that doses of 200 and 400 mg/kg chamomile extract significantly increased the latency of entering the dark chamber and also doses of 100, 200, and 400 mg/kg chamomile extract significantly reduced the time of remaining in the dark chamber of the Shuttle Box in the remembering phase at the level of P≤0.001 compared to the control group. Conclusions: The results of this study showed that chamomile extract strengthens avoidance memory in rats. This effect is probably due to flavonoids, antioxidants and phytoestrogens.
Volume 21, Issue 4 , September and October 2014, , Pages 613-620
Abstract
Background: Pain is an unpleasant sensory experience resulted from tissue damage. Due to the side effects of analgesic medicines; the study was conducted to investigate the influence of music on severity of pain.
Materials and Methods: this experimental study was carried out on 24 adult male rats divided ...
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Background: Pain is an unpleasant sensory experience resulted from tissue damage. Due to the side effects of analgesic medicines; the study was conducted to investigate the influence of music on severity of pain.
Materials and Methods: this experimental study was carried out on 24 adult male rats divided into a control group and two case groups. In the control group, formalin 2.5% injected into the right sole paw and experimental groups were affected to the Adagio calm and Allergo music from 20 minutes prior of formalin injection to the end of the experiment and based on formula of pain scores. Severity of pain was measured every 5 minutes over one hour. Data were analyzed by means of spss.18 and statistical ANOVA and Tukey tests were used.
Results: findings indicated that Adagio calm music can significantly reduce the acute (P≥ 0.05)and chronic pain(P≥ 0.001), and also allergo music led to a decrease in chronic pain (P≥ 0.001).
Conclusions: this is highly likely that music relieve pain through neural pathways, dopaminergic and opioidergic systems of the brain.
Hadi Tavakkoli Kazeroni; Seyyed Ebrahim Hosseini; Mehrdad Shariati
Volume 21, Issue 3 , July and August 2014, , Pages 514-521
Abstract
Background: Hops (Humulus lupulus L.) has industrial and medical applications and is used in the treatment of several diseases. Infertility is a medical important issue that its treatment with chemical medicines has various side effects. Due to fewer side effects of herbal medicines, yet little research ...
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Background: Hops (Humulus lupulus L.) has industrial and medical applications and is used in the treatment of several diseases. Infertility is a medical important issue that its treatment with chemical medicines has various side effects. Due to fewer side effects of herbal medicines, yet little research has been done on the hops. Therefore, this study aimed to evaluate the effect of hops extract on sexual hormone levels and sexual dynastic cells in Syrian adult male mice.
Materials and Methods: This experimental study was performed on 40 Syrian adult male mice that were divided into 5 groups of 8: two controls groups and three experimental groups receiving various doses of hops extract (50, 100 and 150 mg/kg). Administrations were done by gavage for 35 days. At the end of the treatment period, blood samples were taken from the heart of animals and testosterone, estrogen and progesterone levels was measured. Also, after isolation of mouse testis, the number of spermatogonia, spermatocytes and spermatid were counted. Data were analyzed using ANOVA and Tukey statistical tests.
Results: The results showed that hop caused a significant increase in estrogen and testosterone levels and spermatogonia and spermatocytes cells number; but has no effect on progesterone levels.
Conclusion: Hops extract, possibly by having phytoestrogen compounds and by stimulating LH secretion, increases estrogen and testosterone levels, and spermatogonia, spermatocytes and spermatid cells number. Therefore, further investigation on hops can utilize to help infertile men.
Seyyed Ebrahim Hosseini
Volume 20, Issue 3 , September and October 2013, , Pages 359-366
Abstract
Introduction: Nicotine is the alkaloid used by millions of people around the world through smoking and at the same time these people also exposed to some kind stressors that effect on body including endocrine system. The aim of this study was to investigate the interference of nicotine and immobility ...
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Introduction: Nicotine is the alkaloid used by millions of people around the world through smoking and at the same time these people also exposed to some kind stressors that effect on body including endocrine system. The aim of this study was to investigate the interference of nicotine and immobility stress on plasma levels of hormones of ACTH and corticosterone in mature male rats.
Method: In this empirical research study, we used 70 mature male Wistar rats were enrolled as the control, sham and experiment groups randomly. 3 experimental groups of different types received doses of 0.3, 0.5 and 1 mg/kg BW nicotine, and 2 experimental group, one received for 2 hours immobility stress and other for 2 hours immobility stress and other plus nicotine with doses of 0.3 mg/kg BW and the sham group received 1cc of saline intraperitoneally for 5 days. 24 hours after the last injection, mice were bled from the heart and were measured hormones ACTH and corticosterone levels. The data were evaluated using ANOVA and Tuky test.
Results: The results showed that the nicotine at different doses and immobility stress and immobility stress with nicotine increases plasma levels of ACTH and corticosterone hormones.
Discussion and conclusion: According to the result of this study it can be concluded that the effect of immobilization stress and nicotine enhances the stimulatory effects of nicotine and stress on corticosterone and ACTH