Safiyeh Ghazzafi; Ahmad Hamta
Volume 21, Issue 1 , March and April 2015, , Pages 122-130
Abstract
Background: Breast Cancer is the most common cancer in the Iranian women. Fortunately, it is a type of preventable and discernible cancer. The hydroethanolic extract of the leaves and flowered twigs of Thymu vulgaris contain various ingrediants in which the Thymol and Carvacrol are the most important ...
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Background: Breast Cancer is the most common cancer in the Iranian women. Fortunately, it is a type of preventable and discernible cancer. The hydroethanolic extract of the leaves and flowered twigs of Thymu vulgaris contain various ingrediants in which the Thymol and Carvacrol are the most important of them. The effects of this anti-cancer in this extract are attributed significantly to these ingrediants . The objective of this research is to study the comparison of the efficiency of hydroethanolic extract and taxol on 4T1 line cells and their effects rate on the induction of apoptosis in cancer cells.
Materials and Methods: In this research, 4T1 line cells were bought from Tehran's Pasteur institute and the hydroethanolic extract is provided by the Soxhlet’s method. Therefore, We obtained Cytotoxicity five different doses of the extract and five different doses of Taxol in 24, 48 and 72 hours and studied the viability of cells by the MTT and Tripan blue methods. Also, in this research the morphologic changes are done by the Hoechst and Propidiumi odied coloring methods.
Results: Extract and taxol-treated cells at different times have indicated distinct differences and dose-related results. Therefore, simultaneous treatment with the extract in comparison with taxol and taxol-treated cell extract can destroy more cells.
Conclusion: The hydroethanolic extract can increase the cytotoxicity Taxol, Thus it maybe be effective in breast cancer remedy and probably by apoptosis Induction may cause cells death.
Javad Maleki; Mitra Kheyrabadi; Safiyeh Sophiyan; Reyhaneh Sabbaghzadeh
Volume 21, Issue 4 , September and October 2014, , Pages 637-645
Abstract
Background: Virus htlv-1 is in the retrovirus family; the virus causing human diseases such as adult T-Cell Leukemia (ATL), HAM / TSP and etc… One of the major proteins in the virus is protease that is essential for virus maturation. Inhibitors that ever made for the protease didn’t show any properly ...
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Background: Virus htlv-1 is in the retrovirus family; the virus causing human diseases such as adult T-Cell Leukemia (ATL), HAM / TSP and etc… One of the major proteins in the virus is protease that is essential for virus maturation. Inhibitors that ever made for the protease didn’t show any properly activity. We have created a new series of inhibitors and with using of computational tools to calculate the way of interactions to the protease protein.
Materials and Methods: The compounds based on the similarity of the original peptide and based on peptidomemiticswere created. Compounds were created by software Hyperchem and optimization act on designed ligand and ligand with the protein crystal structure were performed after separating them. ADME and toxicity characteristics of the compounds were obtained by using Web applications in http://lazar-services.in-silico.ch and http://www.molinspiration.com and docking were performed on them.
Results: The results of studies on ADME designed compounds already showed a good result. Toxicity studies also indicate relatively good results; also the docking results were showed good specificity.
Conclusion: Our studies showed that designed inhibitors can be effective drug-like compounds to inhibiting the protein and therefore use to contrast with this virus.
Reyhaneh Sabbaghzadeh
Volume 20, Issue 5 , March and April 2014, , Pages 659-664
Abstract
Abstract
Introduction: Cholinergic neurons play an important role in muscle contraction, in learning and memory. Choline acetyltransferase is the enzyme that is responsible for the synthesis of acetylcholine and is a specific marker for choiinergic neurons. Computational methods investigate on Choline ...
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Abstract
Introduction: Cholinergic neurons play an important role in muscle contraction, in learning and memory. Choline acetyltransferase is the enzyme that is responsible for the synthesis of acetylcholine and is a specific marker for choiinergic neurons. Computational methods investigate on Choline acetyltransferase enzyme.
Aim: The aim of the present work was to describe and characterize the molecular structure vibrational properties of choline acetyltransferase crystalline-structure. In this work, the structures of a coordination compound modeling the choline acetyltransferase computationally. Thus, it is worthwhile to collect information on their structures by the means of computational chemistry as well.
Materials and Methods: Monte Carlo simulations are based on pair wise additive potentials of the form . In concepts and algorithms of classical MD simulations the atoms of a biopolymer move according to the Newtonian equations of motion. These studies provided insights into the steric, electrostatic, hydrophobic, and hydrogen bonding properties and other structural features influencing the choline acetyltransferasewas
Results: Potential energies for the three force fields of MM+, AMBER and OPLS at Monte Carlo simulation were compared. Geometry of optimized variables of Bond length (B),Bond Angle (A) and Dihedral Angle (D) investigated. The potential energy (kcal/mol) via time (ps) during Molecular Dynamic (MD) simulation at300 K in gas (R2 = 0.7656) and water (R2 = 0.9794) environments studied to stabilized structure of choline acetyltransferase accepted.
Conclusion: These results also were revealed that the solvation of Choline acetyltransferase molecule is the major component for the interaction potential energy and it was clearly shown that the role of the solute-solvent interactions is more pronounced in Choline acetyltransferase molecule and it’s active site solvation
Mohammadtaghi Shakeri; Mohammad Sadegh Marvi; Hamidreza SafaBakhsh; Parastoo Jamili; Reyhaneh Bazargani
Volume 20, Issue 4 , January and February 2014, , Pages 464-471
Abstract
Background: Human T-lymphotropic virus (HTLV), a retrovirus, is the causative agent of adult T-lymphocytic leukemia (ATL) and tropical spastic paraparesia (TSP). HTLV-1 is transmitted mainly through blood transfusion. Sabzevar is an endemic region for HTLV infection; hence it is essential to detect the ...
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Background: Human T-lymphotropic virus (HTLV), a retrovirus, is the causative agent of adult T-lymphocytic leukemia (ATL) and tropical spastic paraparesia (TSP). HTLV-1 is transmitted mainly through blood transfusion. Sabzevar is an endemic region for HTLV infection; hence it is essential to detect the prevalence of HTLV1/2 infection in the blood donors of this city.
Materials and Methods: In this cross-sectional study, blood samples of all donors referring to Sabzevar Blood Transfusion Center from 2007 to 2008 were screened for HTLV infection using ELISA, and positive samples were confirmed by western blotting. Data were analyzed in SPSS software version 17.0 using Chi squared and Student's t-test.
Results: The results showed that 56 of donors (44 male: 78.6% and 12 female: 21.4%) were infected to HTLV, with average age 40.27±10.02. The prevalence of HTLV infection among all donors was 0.26%. There was a significant correlation between age, marital status, times of blood donation and educational levels and HTLV infection.
Conclusions: Regarding lower rate of infection among younger and regular blood donors and donors with higher educational levels, it should be considered to select donors from this population.
Reyhaneh Sabbaghzadeh
Volume 20, Issue 3 , September and October 2013, , Pages 331-337
Abstract
Introduction: The insulin-like growth factor naturally exists in the Central Nervous System (CNS) and plays a significant role in cellular multiplication and differentiation during growth and maturation of the brain. These factors are expressed with their bond proteins and their receptors in the damaged ...
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Introduction: The insulin-like growth factor naturally exists in the Central Nervous System (CNS) and plays a significant role in cellular multiplication and differentiation during growth and maturation of the brain. These factors are expressed with their bond proteins and their receptors in the damaged areas of the brain. This indicates the role of IGFs systems in the brain damage.
Objective: Molecular simulation is a direct computational method for studying the structural changes of a wide spectrum of physical and biological issues. Computationally, experimental force fields have various forms in simulation of folding insulin-like growth factor.
Materials and Methods: In this study, the transfer temperature for IGF1 was modulated. The system was balanced and was studied and analyzed through dynamic molecular method within 500 Pico seconds.
Results: Studying the changes occurred in the potential energy of the three force fields showed that Amber force field is better than MM+ and OPLS force field and also MD simulation, at least in this model, is more effective than MC and LD methods.
Conclusion: Low temperatures make the structure more stable while high temperatures are on the contrary.
Abbas Ali Gaeini; Fatemeh Shabkhiz; Ali Samadi; Maryam Khalesi; Fateme Tork
Volume 18, Issue 3 , September and October 2011, , Pages 198-205
Abstract
Background and Purpose: It has been suggested that intracellular adhesion molecules (sICAM-1)، as a new inflammatory marker، are more precise and sensitive markers in predicting cardiovascular diseases، compared with traditional predictive markers. The purpose of this investigation was to study the ...
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Background and Purpose: It has been suggested that intracellular adhesion molecules (sICAM-1)، as a new inflammatory marker، are more precise and sensitive markers in predicting cardiovascular diseases، compared with traditional predictive markers. The purpose of this investigation was to study the effect of discontinuous endurance exercise on sICAM-1 and lipid profile (LDL-C، HDL-C، TG، and TC) of non-athletic male students and to survey the correlation between changes of these variables. Material and methods: This experimental study involved twenty non-athletic male students who signed the written informed consent، and were randomly assigned into experimental (n=12) and control (n=8) groups. The exercise protocol was a graded program، to be performed for 8 weeks three times per week with specific intensity and duration. Blood samples were taken before the first session and after the last session، with 14 hours fasting. Data were analyzed in SPSS 16 using independent and dependent t-test، and Pearson correlation coefficient to determine the correlation of the variables. Level of statistical significance was set at α>0.05. Results: Eight weeks of discontinuous endurance exercise caused 14% decline in sICAM-1 (p=0.0001)، 6% in TC (p=0.001)، 10% in LDL-C (p=0.0001)، 40% in TG (p=0.0001) and 23% increase in HDL-C (p=0.001) in the experimental group. However، there were no significant correlation between sICAM-1 alteration and changes of traditional predictors of cardiovascular diseases (LDL-C، HDL-C، TG، and TC) (p>0.05). Conclusion: Eight weeks of discontinuous endurance exercise may reduce sICAM-1 and improve the lipid profile. These changes can play effective roles in the prevention، control and treatment of atherosclerosis.
SM TAVANGAR; MB ARDESHIR LARIJANI; A MAHTA; V HAGHPANAH; A LASHKARI; R HESHMAT
Volume 13, Issue 2 , July and August 2006, , Pages 99-104
Abstract
Background and Purpose: Pituitary adenomas without clinically active hypertension are termed as non-functioning adenoma (NFPA). These tumors represent about one quarter of all pituitary tumors. Immunohistochemical methods are the gold standard methods of identifying them. The Ki-67 is a nuclear antigen ...
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Background and Purpose: Pituitary adenomas without clinically active hypertension are termed as non-functioning adenoma (NFPA). These tumors represent about one quarter of all pituitary tumors. Immunohistochemical methods are the gold standard methods of identifying them. The Ki-67 is a nuclear antigen detected by the monoclonal antibody MIB-1, whose labeling index (LI) is considered as a marker of normal and abnormal cell proliferation.
Methods and Materials: In this clinicopathological study, 85 cases of NFPAs were analyzed immunohistochemically.
Results: MIB-1 LI was measured in all surgical specimens, which was higher than 5% only in 5 cases. Also, 18 cases were immunoactive to one or two adenohypophysial hormones.
Conclusion: NFPAs comprise several pathologically different types of tumors, some of which are potentially hormone producing tumors. However, probable defects in hormone secretion may be the cause of not increasing the adenohypophysial hormone levels in the serum and consequent syndromes.