Roya Hedayati Kashka; Saeed Zavareh; Taghi Lashkabolouki
Volume 23, Issue 4 , September and October 2016, , Pages 590-599
Abstract
Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.Materials & Methods Preantral follicles were isolated ...
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Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.Materials & Methods Preantral follicles were isolated mechanically from 14- to 16-day-old mice (n=123) and divided into control (n=78) and ubiquinone treated groups (n=45). Preantral follicles were cultured in the presence or absence (control) of 50 µM ubiquinone. Ovulation was induced at 12th day. The rates of growth, survival, antrum formation, ovulation, and MII oocytes were evaluated. Separately, malondialdehyde (MDA) as a biomarker of lipid peroxidation was assessed at different time points of 24, 48, 72, and 96 h. Statistical analysis was performed by Independent t test through using SPSS.Results The growth and survival rate of ubiquinone treated preantral follicles was significantly higher than those of the control group. The rates of antrum formation, ovulation, and MII oocytes in the presence of ubiquinone were significantly higher than those of the control group. Whereas, MDA levels of ubiquinone treated preantral follicles was significantly lower compared with that of the control group.Conclusion Supplemented culture medium with ubiquinone improves the development of preantral follicles by reducing the lipid peroxidation.
M ESFAHANI; L HAGH NAZARI; MR SAFARI; N SHEYKH; M KOURKI
Volume 14, Issue 2 , July and August 2007, , Pages 89-94
Abstract
Background and purpose: Oxidative stress is a reaction characterized with an imbalance between Oxidative and antiocidative agents. Lipid perocidation is one of the widely-known biological effects of oxid Oxidative stress. In hemodialysis patients, lipid peroxidation is likely to increase due to free ...
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Background and purpose: Oxidative stress is a reaction characterized with an imbalance between Oxidative and antiocidative agents. Lipid perocidation is one of the widely-known biological effects of oxid Oxidative stress. In hemodialysis patients, lipid peroxidation is likely to increase due to free radicals release from various sources. This study is therefore, intended to determine the effect of hemodialysis on lipid peroxidation changes in hemodialysis patients.
Methods and Materials: In this descriptive-analytical study, samples (17 men and 10 women) were selected through convenient sampling; they had a history of 6-12 month hemodialysis. Malondialdehyde (MDA), an index for lipid peroxidation, was measured by TBA standardized method. Relevant statistical tests were used for data analysis.
Results: Mean MDA before and after hemodialysis were 4.17±1.24 and 4.98±1.2 mmol/l respectively; and the difference was significant. Also, a significant difference was found to exist between male and female mean MDA before and after hemodialysis.
Conclusion: The findings revealed that oxidative stress manifests as increased lipid peroxidation after hemodialysis. Therefore, due to the important role of oxidative stress in causing and developing hemodialysis complication, appropriate measures can be taken to inhibit these reactions in order to take effective steps in patients' recovery.