genetics
Helmah Kargar; Maryam Peymani
Volume 29, Issue 3 , September and October 2022, , Pages 318-329
Abstract
Background: Changes in the SET1B gene expression, can directly affect the incidence and progression of cancer. The gene encoding lncRNA LIMT is transcribed as antisense in the opposite direction to SET1B. The aim of this study was to investigate the expression of lncRNA LIMT and SETD1B in ...
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Background: Changes in the SET1B gene expression, can directly affect the incidence and progression of cancer. The gene encoding lncRNA LIMT is transcribed as antisense in the opposite direction to SET1B. The aim of this study was to investigate the expression of lncRNA LIMT and SETD1B in tumor tissues compared to adjacent normal in colorectal cancer and the relation between these two genes is related to the clinical features of tumor tissues.
Materials and methods: After collecting 40 tumor and adjacent normal tissues, Total RNA extraction and cDNA synthesis were performed. Then the expression levels of the desired genes in tumor and normal tissues was compared. Finally, the obtained results were statistically analyzed by Prism software.
Results: The expression level of SETD1B increased 1.8 fold changes in tumor samples (p = 0.01103) while the expression level of lncRNA LIMT in tumor tissue did not change significantly compared to normal tissue (p = 0.5391). In addition, the expression levels of SET1B and lncRNA LIMT in the two age groups over 60 years and under 60 years in tumor tissues did not change significantly. ROC analysis also showed that SETD1B with AUC = 0.336 and CI = 0.8771 - 0.9902 can separate the patient population from the healthy and can help diagnose colorectal cancer.
Conclusion: According to the results of this study, it can be said that SETD1B is increased in tumor tissue and can be used as a biomarker for colorectal cancer.
Anatomy, Histologyو Embryology
elham hoveizi; Kiavash Hushmandi
Volume 29, Issue 3 , September and October 2022, , Pages 330-343
Abstract
Introduction: In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and ...
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Introduction: In recent years, researchers have considered the anticancer activity coumarins, due to their powerful biological activity and poor toxicity that can neutralize the side effects induced by radiotherapy. The aim of this study was to investigate the cytotoxic effects of coumarin on HT-29 and A549 cancer cells.
Materials and Methods: In this experimental study, the stoke of coumarin was prepared and then for 1,3, and 5 days at concentrations of5, 10, 15, 20, and 25 μM the cells were treated and evaluated on viability days and morphology of the cells indefinite days. The IC50 concentration of coumarin was calculated using MTT assay in two cell lines. Also, the expression of the involved genes in apoptosis such as Bax, Bad, and Bcl-2 was evaluated by the qRT_PCR method. Data were analyzed by a one-way ANOVA test.
Results: The results showed that coumarin reduced the viability and proliferation of HT-29 and special A549 cells by dose and time significantly (P≤0.001), as well as the viability rate of cells in treated cells on the fifth day, significantly decreased compared to the control group (P <0.05). Morphological changes such as reduced chromatin density, cell turnover were also noticeably observed in the cells. Also, molecular results showed that coumarin could significantly increase the expression of Bax, Bad genes and decrease the expression of Bcl-2 gene expression. That these genetic changes in A549 cells were significantly greater than HT-29.
Conclusion: Coumarin is capable of anti-proliferative activity and induces apoptosis effectively against colon and lung cancer cells.
MR MOHAJERI; R GOLMOHAMMADI; SM ZARGARIAN
Volume 14, Issue 3 , September and October 2007, , Pages 141-146
Abstract
Background and purpose: Genetic damages and dietary habits play important parts in colorectal cancer (CRC). p53 protein, a product of p53 gene, is the most important tumor suppressor.
The rate of p53 mutation and expression has been variously reported across anatomical regions. p53 protein has a short ...
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Background and purpose: Genetic damages and dietary habits play important parts in colorectal cancer (CRC). p53 protein, a product of p53 gene, is the most important tumor suppressor.
The rate of p53 mutation and expression has been variously reported across anatomical regions. p53 protein has a short half-life which tends to increase with mutation and is likely to be traced by immunohistochemistry. This study is intended to determine the p53 protein stability by pathological parameters across different areas in CRC.
Methods and Materials: This descriptive analytical research was conducted on 80 CRC cases admitted to Hospital in Isfahan, Iran from 2003 to 2007. p53 expression was detected by immunohistochemical methods in the samples after fixation, tissue processing and antigen retrieval. The obtained data were analyzed using chi-square.
Results: of the 80 specimens investigated, p53 protein stability was observed in 27 specimens (34%). No significant relationships were observed between p53 protein stability and tumor staging, differentiation and anatomical regions (colon and rectum) but the relationship between protein stability and mutation was significant.
Conclusion: p53 protein stability was observed in many mutated samples. Therefore, p53 protein detection in Cancer cases can be considered an important symptom of mutation signifying the prognosis and progress of cancer.
M NIKBAKHT; R GOLMOHAMMADI
Volume 13, Issue 1 , March and April 2006, , Pages 7-13
Abstract
Background and purpose: Colorectal cancer is one of the most important common cancers all over the world. Its prevalence varies with geographical distribution. Its multifactorial cause may include environmental, genetic and dietary origins. P53 is the most important tumor suppressive gene. P53 exon 6 ...
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Background and purpose: Colorectal cancer is one of the most important common cancers all over the world. Its prevalence varies with geographical distribution. Its multifactorial cause may include environmental, genetic and dietary origins. P53 is the most important tumor suppressive gene. P53 exon 6 mutation with protein overexpression is reported in different areas. This study is intended to determine the relationship between P53 exon 6 mutation with protein overxpression and prognosis in colorectal cancer.
Methods and Materials: This study was conducted on 80 cases of Colorectal cancers, admitted to Isfahan hospitals in Isfahan, Iran from 1382 (2003) to 1385(2006). DNA was extracted by phenol chloroform isoamil alcohol at the Genetic Department of the faculty of Medicine. Exon 6 of the P53 gene was amplified using primers in a PCR assay. After gel electrophoresis by SSCP method, exon 6 mutations were determined. P53 protein overexpression was determined in cases by immunohistochemistry. Chi-square test was used for data analysis.
Results: Mutations were observed in 12 cases (15%); in 10 cases, mutations demonstrated protein overexpression as well. No significant relationship was observed between P53 exon 6 mutation and its overexpression (p>0.05).
Conclusion: The study revealed that not all P53 exon 6 mutations were accompanied by P53 overexpression. Therefore, besides immuno histochemistry, we need PCR-SSCP or sequencing for diagnosis.