Physical Education
fatemhe parsifar; Majid Vahidian-Rezazadeh; Mohsen Saravani; Hossien Nakhaie
Abstract
Introduction: Alzheimer's disease is the most common form of dementia, which is a fatal neurological disorder. The purpose of this study was to investigate the effect of eight weeks of endurance training and Ginkgo biloba supplementation on the neurotrophic factor derived (BDNF) level from the hippocampus ...
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Introduction: Alzheimer's disease is the most common form of dementia, which is a fatal neurological disorder. The purpose of this study was to investigate the effect of eight weeks of endurance training and Ginkgo biloba supplementation on the neurotrophic factor derived (BDNF) level from the hippocampus brain in the Alzheimer's experimental model.
Materials and Methods: This research was of laboratory type. 50 male Wistar rats weighing 250±20 grams were prepared and divided into seven groups including healthy control, Alzheimer's control, intermittent endurance exercise, Ginkgobiloba supplement, Ginkgobiloba supplement+exercise, shock Surgery, and placebo. To induce Alzheimer's disease in the patient groups, amyloid-beta solution was injected into both sides of the ventricles of the rats' brains. For eight weeks, the exercise groups ran on a treadmill 5 days a week, and the supplemented groups received Ginkgo biloba solution. 24 hours after the last exercise session, the hippocampus tissue was extracted and the BDNF protein level was measured using ELISA method.SPSS software version 22 was used to analyze the data at a significant level (P<0.05).
Results: In the comparison between the groups, the level of BDNF factor was higher in the training+supplement group than in the healthy control, Alzheimer's control, surgical shock, and placebo groups, no increase was observed in the training and supplement alone groups, and the level of the factor was also decreased in the surgical shock and placebo groups.
Conclusion: Intermittent endurance exercise and Ginkgo Biloba supplement consumption at the same time can be an effective treatment to increase the level of BDNF to slow down the process of Alzheimer's disease.
immunology & Biochemistry
mozhgan naderi kotaki; azizeh asadzadeh; fatemeh heidaryan
Volume 27, Issue 5 , November and December 2020, , Pages 594-602
Abstract
Introduction: Alzheimer’s disease is the most common cause of dementia in the elderly and is characterized by loss of memory and other cognitive functions. The acetylcholinesterase enzyme (ACHE) with hydrolysis of the neurotransmitter acetylcholine in the cholinergic synapses of the brain plays ...
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Introduction: Alzheimer’s disease is the most common cause of dementia in the elderly and is characterized by loss of memory and other cognitive functions. The acetylcholinesterase enzyme (ACHE) with hydrolysis of the neurotransmitter acetylcholine in the cholinergic synapses of the brain plays a role in ending the neural message. One of the methods used to control the progression of Alzheimer’s disease is the administration of cholinesterase inhibitors, especially plant- originated medicines. The aim of this study was to study bioinformatics and obtain the level of inhibitory effect of acetylcholinesterase enzyme by hydro-alcoholic extract of aerial organs of Thymus vulgaris. Materials and Methods: In order to investigate how the compounds are attached to the active site of the enzyme, first, Docking studies were conducted using AutoDock 4.2 software, the hydro-alcoholic extract of Thymus vulgaris was measured at concentrations 2, 1, 0 .5, 0.25 mg/ml in order to determine its inhibitory effect on acetylcholinesterase. Results: The compounds present in the extract were able to occupy the active site of the enzyme with a binding energy level of -4.12 to -6.58 kcal / mol. According to the laboratory results, concentrations of 2mg/ml were 46.99% and IC50 = 2.03 mg/ml. Conclusion: The results of in silico and in vitro studies indicate that the extract of this plant contains effective inhibitory compounds, and trying to use these compounds is a suitable topic for future research with the aim of acquiring pharmacologically inhibitors.