Biotechnology & nanotechnology
Aysan Ghahremani; Ebrahim Ganji_Moghadam; Maryam Tatari; Susan Khosroyar
Volume 28, Issue 2 , May and June 2021, , Pages 274-285
Abstract
Introduction: Paneer-boot, with its antifungal and antibacterial effects, blood sugar and cholesterol-lowering, immune system, and antioxidant activity, has recently received pharmacologists' attention due to its high compatibility. Due to difficult ecological conditions, it can grow in the country's ...
Read More
Introduction: Paneer-boot, with its antifungal and antibacterial effects, blood sugar and cholesterol-lowering, immune system, and antioxidant activity, has recently received pharmacologists' attention due to its high compatibility. Due to difficult ecological conditions, it can grow in the country's tropical regions such as Sistan and Baluchestan Province .
Materials & Methods: This study was designed in a completely randomized design with four replications under laboratory conditions at Khorasan Razavi Agricultural and Natural Resources Research and Education Center. Concentrations of 0, 2, 4, 6, 8, and 10 bar PEG were used to induce the effect of drought stress on MS medium containing BAP on secondary traits of cheese .
Results: The results showed that the drought stress significantly increased antioxidant enzymes such as catalase, peroxidase, ascorbate peroxidase, proline, phenol, and flavonoid content membrane leakage. On the other hand, it resulted in a significant decrease in the relative humidity content, potassium ion content and potassium to sodium ratio. Also, most of the studied traits had a significant correlation. Conclusion: The results showed that the Paneer-boot plant had acceptable tolerance to induction of drought stress with polyethylene glycol. Enzymatic and non-enzymatic mechanisms had a significant role in reducing the adverse effects of drought stress. On the other hand, drought stress increased some metabolites, such as phenol and flavonoids, which have medicinal uses .
Microbiology
Amir Hadi Chegeni; Faranak Hadi; Mojgan kowsari3; Maryam Zare; Abdolnaser Gholami Mohammadi
Volume 26, Issue 3 , September and October 2019, , Pages 383-391
Abstract
Background: Emergence of drug resistant strains and the limited use of antifungal drug due to possible toxic effects on humans, the high cost of their production and environmental pollution has made the search for novel bio-antifungal compounds a necessity. Microorganisms, particularly soil bacteria ...
Read More
Background: Emergence of drug resistant strains and the limited use of antifungal drug due to possible toxic effects on humans, the high cost of their production and environmental pollution has made the search for novel bio-antifungal compounds a necessity. Microorganisms, particularly soil bacteria produce a wide range of active antimicrobial substances that some of them have antifungal properties. The purpose of this study is isolation and identification of the bacteria affecting the growth of fungi from Khorramabad agricultural soil.Materials and Methods: Agricultural soils were collected from different areas of Khorramabad, Lorestan Province. The bacteria were isolated using nutrient broth and nutrient agar, then, the antifungal activity of their extracts and supernatant in the SCA media on several species of fungi was studied applying disk diffusion method.Results: Four strains showing a remarkable antifungal effect against some fungi, including Aspergillus niger, Aspergillus flavus, Aspergillus fumigatus and Candida albicans. These isolates were identified by biochemical features and 16S rRNA sequence. Analysis of their sequencing through BLAST in NCBI data bank showed that these bacterial similarly closed to Pseudomonas sp. and Acinetobacter sp. genus.Conclusion: This study was the first report of antifungal analysis of soil isolated bacteria from Khorramabad. These bacteria could be a potential candidate for biocontrolling pathogenic fungi.The extraction, identification and development of their inhibitory metabolites can be used in the design and production of antifungal agents.