Biotechnology & nanotechnology
Marzieh Varasteh Shams; Farhad Nazarian-Firouzabadi; Ahmad Ismaili
Volume 26, Issue 4 , November and December 2019, , Pages 404-412
Abstract
New and effective antimicrobial agents are inevitable because of the increased use of antibiotics and spread of microbial resistance. The expression of antimicrobial peptides that are part of the inherent immune system of all organisms, is a novel approach of confronting a wide range of pathogens. Among ...
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New and effective antimicrobial agents are inevitable because of the increased use of antibiotics and spread of microbial resistance. The expression of antimicrobial peptides that are part of the inherent immune system of all organisms, is a novel approach of confronting a wide range of pathogens. Among the wide range of antimicrobial peptides, Dermaseptin B1 is a potent cationic peptide with strong antimicrobial activity. In this study, Dermaseptin B1 (DrsB1) coding sequence from Phyllomedusa bicolor frogs was fused to a tandem repeat of a chitin-binding domain (CBD) from Cladosporium fulvum Avr4 effector protein and expressed in tobacco Hairy Roots (HRs). The expression of recombinant (CBD)2-DrsB1 peptide was confirmed, using molecular methods and its antimicrobial activity was evaluated against Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus, Enterococcus faecalis and Bacillus subtilis bacteria. The results of antimicrobial activity analysis demonstrated that the recombinant protein had a significant (P
Ali Asghar Ghaderi; Baratali Fakheri; Nafise Mahdinezhad; Saeede Saeedi
Abstract
Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus ...
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Backgrounds The current study aimed at investigating the antimicrobial effects of Vulgaris thymus ethanol extract on the important human pathogens.Methods & Materials The thymus alcoholic extract was provided based on a standard protocol. The following standard bacteria were also provided: Streptococcus pyogenes ATCC 19615, Streptococcus pneumoniae ATCC 49619, S. saprophyticus ATCC15305, Hafnia alvei ATCC 51873, Acinetobacter baumannii ATCC 19606, Enterococcus faecalis ATCC 29212, Proteus mirabilis ATCC 35659, Serratia marcescens ATCC 274, Staphylococcus aureus ATCC 25923.The minimum inhibitory concentration (MIC) of thymus ethanol extract on the bacteria was determined. The linear sensitivity of the bacteria against some antibiotics was also measured by the standard method of Kirby-Bauer disk diffusion. Results The results showed that the thymus leaf alcoholic extract inhibited the bacterial growth in the evaluated concentrations. The MIC of 6/25 ppm was observed effective against S. pneumoniae, H. alvei, P. mirabilis, and S. marcescens.Conclusion The results of the current study indicated the high effect of thymus alcoholic extract on the pathogenic bacteria. However, the medicinal application of plants requires further investigations.