Physiology & Pharmacology
Roya Kaveh; Mohammad Amin Edalatmanesh
Volume 29, Issue 2 , July and August 2022, , Pages 268-280
Abstract
Introduction: Monosodium glutamate (MSG) induced excitotoxicity leads to oxidative stress in different areas of the brain. The aim of this study was to evaluate the neuroprotective effect of coenzyme Q10 (Co-Q10) on amelioration of short-term and long-term memory and oxidative stress parameters in MSG-treated ...
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Introduction: Monosodium glutamate (MSG) induced excitotoxicity leads to oxidative stress in different areas of the brain. The aim of this study was to evaluate the neuroprotective effect of coenzyme Q10 (Co-Q10) on amelioration of short-term and long-term memory and oxidative stress parameters in MSG-treated rats.Material and Methods: In this experimental study, 40 adult male Wistar rats were randomly divided into 4 groups including control, MSG, MSG + Q10-10 and MSG + Q10-20. MSG gavage (4 mg / kg) and coenzyme Q10 injection at doses of 10 and 20 mg / kg (intraperitoneally; i.p.) were performed for 4 weeks. Then, short-term working memory was assessed using the Y maze and long-term avoidance memory was performed with the shuttle box. Hippocampal level of catalase (CAT) and total antioxidant capacity (TAC) were determined by ELISA method and malondialdehyde (MDA) content was determined by thiobarbituric acid method.Results: In MSG group, there was a significant decrease in alteration behavior, increased latency time to the dark room of the shuttle box, decreased CAT, TAC expression and increased MDA compared to the control group (p <0.05). While in the Q10 treated groups, there was an increase in working and avoidance memory, an increase in CAT and TAC expression and a decrease in MDA in the hippocampus compared to the MSG group (p˂0.05).Conclusion: It seems that Co-Q10 ameliorates MSG induced neurotoxicity and cognitive symptoms through preventing oxidative stress in the hippocampus.
Roya Hedayati Kashka; Saeed Zavareh; Taghi Lashkabolouki
Volume 23, Issue 4 , September and October 2016, , Pages 590-599
Abstract
Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.Materials & Methods Preantral follicles were isolated ...
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Background Oxidative stress is unavoidable during in vitro culture. The present study aimed to investigate the effect of ubiquinone as a potent antioxidant on lipid peroxidation and development rate of mice in vitro cultured preantral follicles.Materials & Methods Preantral follicles were isolated mechanically from 14- to 16-day-old mice (n=123) and divided into control (n=78) and ubiquinone treated groups (n=45). Preantral follicles were cultured in the presence or absence (control) of 50 µM ubiquinone. Ovulation was induced at 12th day. The rates of growth, survival, antrum formation, ovulation, and MII oocytes were evaluated. Separately, malondialdehyde (MDA) as a biomarker of lipid peroxidation was assessed at different time points of 24, 48, 72, and 96 h. Statistical analysis was performed by Independent t test through using SPSS.Results The growth and survival rate of ubiquinone treated preantral follicles was significantly higher than those of the control group. The rates of antrum formation, ovulation, and MII oocytes in the presence of ubiquinone were significantly higher than those of the control group. Whereas, MDA levels of ubiquinone treated preantral follicles was significantly lower compared with that of the control group.Conclusion Supplemented culture medium with ubiquinone improves the development of preantral follicles by reducing the lipid peroxidation.