Biotechnology & nanotechnology
Zeinab Noori; Akbar Safipour Afshar
Volume 26, Issue 4 , November and December 2019, , Pages 527-534
Abstract
Aim: The characteristics of cancer cells that differentiate them from normal cells can be attributed to the increase of the ornithine decarboxylase enzyme (ODC), which has a very important role in the metabolism of polyamines in cancer cells. In this research, we tried to study the effects of cytotoxicity ...
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Aim: The characteristics of cancer cells that differentiate them from normal cells can be attributed to the increase of the ornithine decarboxylase enzyme (ODC), which has a very important role in the metabolism of polyamines in cancer cells. In this research, we tried to study the effects of cytotoxicity of Rhubarb extract on three different solvents distilled water, ethanol and n-hexane on MCF-7 cell lines. Also, the rate of expression of the ODC1 gene as one of the genes involved in breast cancer was studied under the influence of the extract of this plant.Materials and Methods: MCF-7 breast cancer cells were cultured in DMEM medium. Subsequently, they were subjected to increasing concentrations of extract from 0-200 μg/mL. The cytotoxic activity of the extract was investigated using MTT assay. The quantitative analysis of the ODC1 gene was performed using the Real Time-PCR.Results: Cell line mortality increases significantly in a concentration-dependent manner. In the rhubarb extracts, the most cytotoxicity was observed in 72 hours. Changes in ODC1 gene expression in MCF-7 cell lines treated with different concentrations of rhubarb extract indicate a decrease in gene expression with increasing concentrations of extracts.Conclusion: The results of this study indicate that Rhubarb extract has a potential influence on ODC1 gene expression in breast cancer cells and cause inhibition of cancer cell proliferation.
Ali Salehi; Nabi Shariatifar; Aftab Salehi; Alireza Mohammadzadeh
Volume 23, Issue 3 , July and August 2016, , Pages 430-437
Abstract
Background Food-borne pathogens are a group of microorganisms that cause food-borne illness, and the research for finding effective natural products against the infection arisen from these microorganisms is necessary. The purpose of this study was to determine antimicrobial effect of aqueous and ethanol ...
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Background Food-borne pathogens are a group of microorganisms that cause food-borne illness, and the research for finding effective natural products against the infection arisen from these microorganisms is necessary. The purpose of this study was to determine antimicrobial effect of aqueous and ethanol extracts of Rheum ribes on some important foodborne bacteria.Materials & Methods After collecting the leaves and stalks of rhubarb in the spring, its different concentrations of 50, 75, 100, 125, and 150 mg/mL were prepared. Then, antibacterial effect of aqueous and ethanol extracts were determined by the disk diffusion and serial dilution methods.Results Findings showed that aqueous and ethanolic extracts of stalks and leaves of rhubarb have good inhibitory effect on S. aureus and E. coli. The minimum inhibitory concentration (MIC) of the ethanolic leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤75, ≤50, ≤100, and ≤75, respectively, and MIC of the aqueous leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤100, ≤100, ≤125, and ≤100, respectively. The minimum bactericidal concentration of the ethanolic leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤100, ≤75, ≤150, and ≤125, respectively, and aqueous extracts did not have bactericidal effects.Conclusion Extracts of rhubarb leave and stalk showed a good antimicrobial activity against food-borne pathogens. Therefore, they can be used in food preservation systems to inhibit the growth of these bacteria and improve food quality and safety.