Ali Salehi; Nabi Shariatifar; Aftab Salehi; Alireza Mohammadzadeh
Volume 23, Issue 3 , July and August 2016, , Pages 430-437
Abstract
Background Food-borne pathogens are a group of microorganisms that cause food-borne illness, and the research for finding effective natural products against the infection arisen from these microorganisms is necessary. The purpose of this study was to determine antimicrobial effect of aqueous and ethanol ...
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Background Food-borne pathogens are a group of microorganisms that cause food-borne illness, and the research for finding effective natural products against the infection arisen from these microorganisms is necessary. The purpose of this study was to determine antimicrobial effect of aqueous and ethanol extracts of Rheum ribes on some important foodborne bacteria.Materials & Methods After collecting the leaves and stalks of rhubarb in the spring, its different concentrations of 50, 75, 100, 125, and 150 mg/mL were prepared. Then, antibacterial effect of aqueous and ethanol extracts were determined by the disk diffusion and serial dilution methods.Results Findings showed that aqueous and ethanolic extracts of stalks and leaves of rhubarb have good inhibitory effect on S. aureus and E. coli. The minimum inhibitory concentration (MIC) of the ethanolic leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤75, ≤50, ≤100, and ≤75, respectively, and MIC of the aqueous leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤100, ≤100, ≤125, and ≤100, respectively. The minimum bactericidal concentration of the ethanolic leaves and stalks extracts of rhubarb against S. aureus and E. coli were ≤100, ≤75, ≤150, and ≤125, respectively, and aqueous extracts did not have bactericidal effects.Conclusion Extracts of rhubarb leave and stalk showed a good antimicrobial activity against food-borne pathogens. Therefore, they can be used in food preservation systems to inhibit the growth of these bacteria and improve food quality and safety.
Saeedeh Saeedi; Mahta Mazaheri Naeeni; Seyyed Kazem Sabbagh; Safoura Bazi
Volume 21, Issue 4 , September and October 2014, , Pages 596-603
Abstract
Background and aim: Infectious diseases are one of the most important agents of mortality in the world. Due to resistant of some human infectious bacteria to antibiotics, use of plant pharmaceutical herbs to control of these agents is increasing. The aim of this research was to investigate the antibacterial ...
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Background and aim: Infectious diseases are one of the most important agents of mortality in the world. Due to resistant of some human infectious bacteria to antibiotics, use of plant pharmaceutical herbs to control of these agents is increasing. The aim of this research was to investigate the antibacterial effect of aqueous extract of Yarrow and Salvia against ten bacterial strains of human pathogenic bacteria.
Materials and Methods: Aqueous extract from Achillea millefolium and Teucrium polium species were prepared using maceration method and then were concentrated and dried using rotary apparatus. . Ten standard bacterial strains were cultured on Nutrient Broth. Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of plant extract of both extract on bacteria were determined using micro dilution method at four different concentrations (0/62-1/25-2/5-5-10mg/ml).
Results:The results showed that aqueous extract from Achillea millefolium and Teucrium polium species have an inhibition effect on all tested bacteria. Staphylococcus aureus and S. saprophyticus showed the highest and lowest sensitivity to water extract of yarrow respectively. Pseudomonas aeroginosa sereus strain showed high degree of sensitivity whereas S. aureuss and Bacillus aeroginosa strains were less sensitive to extract of salvia.
Conclusion: According to obtained results in this work and other researches in this domain, the Aqueous extracts of Yarraw and Salvia could be introduced as a biocontrol agent against pathogenic micro-organisms. Nevertheless to use each extract of plant for clinical application, chemical analysis of extracts and clinical researches are necessary.