Physiology & Pharmacology
mahnaz davoudi; Hossein azizi; Javad Mirnajafi-Zadeh; saeed semnanian
Volume 27, Issue 5 , November and December 2020, , Pages 630-639
Abstract
Introduction: The neuropeptide orexin is synthetized in the lateral hypothalamus (LH) and is involved in naloxone-precipitated morphine withdrawal syndrome via orexin type 1 receptors (OX1R). Locus coeruleus (LC) is a sensitive site for the expression of somatic aspects of morphine withdrawal. The orexinergic ...
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Introduction: The neuropeptide orexin is synthetized in the lateral hypothalamus (LH) and is involved in naloxone-precipitated morphine withdrawal syndrome via orexin type 1 receptors (OX1R). Locus coeruleus (LC) is a sensitive site for the expression of somatic aspects of morphine withdrawal. The orexinergic and GABA-A-ergic systems are involved in morphine withdrawal syndrome. In this study, the effect of OX1R at the LC neurones on GABA-A-ergic inhibitory system activity in morphine withdrawal syndrome was investigated.
Materials and Methods: Male Wistar rats (14-21 days) were made dependent on morphine (20 mg/kg, i.p., for 7 days). Then the effect of orexin-A on spontaneous and evoked inhibitory post synaptic currents in LC neurons using whole-cell patch clamp recordings was assessed.
Results: The findings of this study indicated that orexin-A through OX1R in the presence of naloxone may induce an inhibitory effect on GABAergic system in the LC neurons. It seems that the orexin-A administration decreased the eIPSCs amplitude in LC neurons. OrexinA decreased spontaneous sIPSCs frequency of LC neurons, but did not change the sIPSCs amplitude in the presence of naloxone.
Conclusion: These findings implicated evidence that orexin-A via OX1R may participate in expression of naloxone-precipitated morphine withdrawal syndrome through decreasing of GABAA receptor activity.
Seyed Mehdi Beheshti Nasr; Mohammad Mohammadzadeh; Hasan Ramshini
Volume 21, Issue 2 , May and June 2014, , Pages 352-361
Abstract
Introduction: Minocycline has anticonvulsant effects. Since some antiepileptic drugs increase the neurotransmitter GABA in the brain, the aim of this study is the effect of minocycline on gene expression of GABAA receptor in hippocampus and piriform brain areas on amygdale kindling acquisition in rat.
Methods: ...
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Introduction: Minocycline has anticonvulsant effects. Since some antiepileptic drugs increase the neurotransmitter GABA in the brain, the aim of this study is the effect of minocycline on gene expression of GABAA receptor in hippocampus and piriform brain areas on amygdale kindling acquisition in rat.
Methods: In this experimental study, three group (24 Wistar rats), after stereotaxic surgery and 1 week recovery period, received kindling stimulations(twice daily at 6 hours interval). Group 1(n=8) did not receive daily kindling stimulations. Group 2 (n=8) received intraperitoneal saline (1ml/kg) and Group 3 (n=8) received intraperitoeneal minocycline (25 mg/kg) 60 min before kindling stimulation and respectively. Two hours after the last stimulation, animals’ brains were removed and the changes of gene expression by γ2 subunit of GABAAreceptor in the hippocampus and piriform cortex were measured and compared with the control group. Data was analyzed using one-way ANOVA and Tukey post hoc tests (P