Microbiology
maryam alsadat zarei; Mahboubeh Madani
Volume 25, Issue 1 , May and June 2018, , Pages 151-160
Abstract
Background: Teucrium polium is a medicinal plant which species have been used for over 2000 years in traditional medicine. The aim of this study was evaluation of the effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in mice tissue. Material and Method: The study was performed ...
Read More
Background: Teucrium polium is a medicinal plant which species have been used for over 2000 years in traditional medicine. The aim of this study was evaluation of the effect of ethanolic extract of Teucrium polium on Candida glabrata colonization in mice tissue. Material and Method: The study was performed on 90 mature male mices (9 per group), that were devided into 10 groups: normal, placebo, 3 treatment group (candida- extract), control positive (Candida glabrata) and control negative (extract). Treatment groups were received 50,100 and 200 mg/kg dosages of extract for 20 days (every other day) by intraperitoneal injection. At the end of injections, homogenized liver, spleen and kidney were cultured on SDA medium and then colony count were performed. Results: The results showed, that liver in 100 mg/kg, spleen and kidney in 200mg/kg haven’t any yeast. Discussion and Conclusion: According to the results, Teucrium polium extract, perhaps can stimulat the immunity against Candida glabrata. Keywords: Teucrium polium, Candida glabrata, liver, spleen, kidney
Farzad Rajaee; Tayyebeh Hadigol; Zivar Salehi; Amir Farzam
Volume 21, Issue 1 , March and April 2015, , Pages 207-215
Abstract
Background and Objectives: Regarding to remarkable growth of population and consequent crowding of it in big cities and immune system sensitivity against environmental agents, the present study aimed to investigate the effects of social stress on spleen of mouse.
Methods and Materials: Thirty adult ...
Read More
Background and Objectives: Regarding to remarkable growth of population and consequent crowding of it in big cities and immune system sensitivity against environmental agents, the present study aimed to investigate the effects of social stress on spleen of mouse.
Methods and Materials: Thirty adult male mice of the NMRI strain were selected at the age of 5-6 weeks and were randomly divided into 2 groups. In control group, 5 animals per cage (3 cages) and in stress group, 15 animals per cage were housed for a period of two months. All animals were anesthetized with an intrapritoneally injection of ketamine and xylazine after two months. The sample of blood from left ventricle of heart was provided by heparinized syringe. Serum samples were used for hormonal study by radioimmunoassay. Spleen was weighted after removing and the samples of spleen were fixed for light microscopic study. The mean numbers of macrophage and megakaryocyte cells were determined. The data has been compared using statistical methods (t-test and Mann-Whitney test).
Results: The mean weight of spleen and corticosterone level of serum was insignificantly reduced in stress group compared to control group. The mean numbers of macrophage cells in stress group were insignificantly increased compared to control group. The results showed that the mean numbers of megakaryocyte cells were significantly reduced between stress and control group(P=0.007).
Conclusions: The results showed that the crowding stress can be resulted in decreased number of megakaryocyte cells whereas no significant effect on macrophage cells, serum cortisol hormone level and weight of spleen were found. However, further studies in this area are needed to confirm it.